Cargando…

Vinculin anchors contractile actin to the cardiomyocyte adherens junction

The adherens junction (AJ) couples the actin cytoskeletons of neighboring cells to allow mechanical integration and tissue organization. The physiological demands of intercellular adhesion require that the AJ be responsive to dynamic changes in force while maintaining mechanical load. These demands...

Descripción completa

Detalles Bibliográficos
Autores principales: Merkel, Chelsea D., Li, Yang, Raza, Qanber, Stolz, Donna B., Kwiatkowski, Adam V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761764/
https://www.ncbi.nlm.nih.gov/pubmed/31483697
http://dx.doi.org/10.1091/mbc.E19-04-0216
_version_ 1783454091125456896
author Merkel, Chelsea D.
Li, Yang
Raza, Qanber
Stolz, Donna B.
Kwiatkowski, Adam V.
author_facet Merkel, Chelsea D.
Li, Yang
Raza, Qanber
Stolz, Donna B.
Kwiatkowski, Adam V.
author_sort Merkel, Chelsea D.
collection PubMed
description The adherens junction (AJ) couples the actin cytoskeletons of neighboring cells to allow mechanical integration and tissue organization. The physiological demands of intercellular adhesion require that the AJ be responsive to dynamic changes in force while maintaining mechanical load. These demands are tested in the heart, where cardiomyocyte AJs must withstand repeated cycles of actomyosin-mediated contractile force. Here we show that force-responsive cardiomyocyte AJs recruit actin-binding ligands to selectively couple actin networks. We employed a panel of N-cadherin-αE-catenin fusion proteins to rebuild AJs with specific actin linkages in N-cadherin-null cardiomyocytes. In this system, vinculin recruitment was required to rescue myofibril integration at nascent contacts. In contrast, loss of vinculin from the AJ disrupted junction morphology and blocked myofibril integration at cell–cell contacts. Our results identify vinculin as a critical link to contractile actomyosin and offer insight to how actin integration at the AJ is regulated to provide stability under mechanical load.
format Online
Article
Text
id pubmed-6761764
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-67617642019-12-16 Vinculin anchors contractile actin to the cardiomyocyte adherens junction Merkel, Chelsea D. Li, Yang Raza, Qanber Stolz, Donna B. Kwiatkowski, Adam V. Mol Biol Cell Articles The adherens junction (AJ) couples the actin cytoskeletons of neighboring cells to allow mechanical integration and tissue organization. The physiological demands of intercellular adhesion require that the AJ be responsive to dynamic changes in force while maintaining mechanical load. These demands are tested in the heart, where cardiomyocyte AJs must withstand repeated cycles of actomyosin-mediated contractile force. Here we show that force-responsive cardiomyocyte AJs recruit actin-binding ligands to selectively couple actin networks. We employed a panel of N-cadherin-αE-catenin fusion proteins to rebuild AJs with specific actin linkages in N-cadherin-null cardiomyocytes. In this system, vinculin recruitment was required to rescue myofibril integration at nascent contacts. In contrast, loss of vinculin from the AJ disrupted junction morphology and blocked myofibril integration at cell–cell contacts. Our results identify vinculin as a critical link to contractile actomyosin and offer insight to how actin integration at the AJ is regulated to provide stability under mechanical load. The American Society for Cell Biology 2019-10-01 /pmc/articles/PMC6761764/ /pubmed/31483697 http://dx.doi.org/10.1091/mbc.E19-04-0216 Text en © 2019 Merkel et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Merkel, Chelsea D.
Li, Yang
Raza, Qanber
Stolz, Donna B.
Kwiatkowski, Adam V.
Vinculin anchors contractile actin to the cardiomyocyte adherens junction
title Vinculin anchors contractile actin to the cardiomyocyte adherens junction
title_full Vinculin anchors contractile actin to the cardiomyocyte adherens junction
title_fullStr Vinculin anchors contractile actin to the cardiomyocyte adherens junction
title_full_unstemmed Vinculin anchors contractile actin to the cardiomyocyte adherens junction
title_short Vinculin anchors contractile actin to the cardiomyocyte adherens junction
title_sort vinculin anchors contractile actin to the cardiomyocyte adherens junction
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761764/
https://www.ncbi.nlm.nih.gov/pubmed/31483697
http://dx.doi.org/10.1091/mbc.E19-04-0216
work_keys_str_mv AT merkelchelsead vinculinanchorscontractileactintothecardiomyocyteadherensjunction
AT liyang vinculinanchorscontractileactintothecardiomyocyteadherensjunction
AT razaqanber vinculinanchorscontractileactintothecardiomyocyteadherensjunction
AT stolzdonnab vinculinanchorscontractileactintothecardiomyocyteadherensjunction
AT kwiatkowskiadamv vinculinanchorscontractileactintothecardiomyocyteadherensjunction