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Vinculin anchors contractile actin to the cardiomyocyte adherens junction
The adherens junction (AJ) couples the actin cytoskeletons of neighboring cells to allow mechanical integration and tissue organization. The physiological demands of intercellular adhesion require that the AJ be responsive to dynamic changes in force while maintaining mechanical load. These demands...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761764/ https://www.ncbi.nlm.nih.gov/pubmed/31483697 http://dx.doi.org/10.1091/mbc.E19-04-0216 |
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author | Merkel, Chelsea D. Li, Yang Raza, Qanber Stolz, Donna B. Kwiatkowski, Adam V. |
author_facet | Merkel, Chelsea D. Li, Yang Raza, Qanber Stolz, Donna B. Kwiatkowski, Adam V. |
author_sort | Merkel, Chelsea D. |
collection | PubMed |
description | The adherens junction (AJ) couples the actin cytoskeletons of neighboring cells to allow mechanical integration and tissue organization. The physiological demands of intercellular adhesion require that the AJ be responsive to dynamic changes in force while maintaining mechanical load. These demands are tested in the heart, where cardiomyocyte AJs must withstand repeated cycles of actomyosin-mediated contractile force. Here we show that force-responsive cardiomyocyte AJs recruit actin-binding ligands to selectively couple actin networks. We employed a panel of N-cadherin-αE-catenin fusion proteins to rebuild AJs with specific actin linkages in N-cadherin-null cardiomyocytes. In this system, vinculin recruitment was required to rescue myofibril integration at nascent contacts. In contrast, loss of vinculin from the AJ disrupted junction morphology and blocked myofibril integration at cell–cell contacts. Our results identify vinculin as a critical link to contractile actomyosin and offer insight to how actin integration at the AJ is regulated to provide stability under mechanical load. |
format | Online Article Text |
id | pubmed-6761764 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-67617642019-12-16 Vinculin anchors contractile actin to the cardiomyocyte adherens junction Merkel, Chelsea D. Li, Yang Raza, Qanber Stolz, Donna B. Kwiatkowski, Adam V. Mol Biol Cell Articles The adherens junction (AJ) couples the actin cytoskeletons of neighboring cells to allow mechanical integration and tissue organization. The physiological demands of intercellular adhesion require that the AJ be responsive to dynamic changes in force while maintaining mechanical load. These demands are tested in the heart, where cardiomyocyte AJs must withstand repeated cycles of actomyosin-mediated contractile force. Here we show that force-responsive cardiomyocyte AJs recruit actin-binding ligands to selectively couple actin networks. We employed a panel of N-cadherin-αE-catenin fusion proteins to rebuild AJs with specific actin linkages in N-cadherin-null cardiomyocytes. In this system, vinculin recruitment was required to rescue myofibril integration at nascent contacts. In contrast, loss of vinculin from the AJ disrupted junction morphology and blocked myofibril integration at cell–cell contacts. Our results identify vinculin as a critical link to contractile actomyosin and offer insight to how actin integration at the AJ is regulated to provide stability under mechanical load. The American Society for Cell Biology 2019-10-01 /pmc/articles/PMC6761764/ /pubmed/31483697 http://dx.doi.org/10.1091/mbc.E19-04-0216 Text en © 2019 Merkel et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License. |
spellingShingle | Articles Merkel, Chelsea D. Li, Yang Raza, Qanber Stolz, Donna B. Kwiatkowski, Adam V. Vinculin anchors contractile actin to the cardiomyocyte adherens junction |
title | Vinculin anchors contractile actin to the cardiomyocyte adherens junction |
title_full | Vinculin anchors contractile actin to the cardiomyocyte adherens junction |
title_fullStr | Vinculin anchors contractile actin to the cardiomyocyte adherens junction |
title_full_unstemmed | Vinculin anchors contractile actin to the cardiomyocyte adherens junction |
title_short | Vinculin anchors contractile actin to the cardiomyocyte adherens junction |
title_sort | vinculin anchors contractile actin to the cardiomyocyte adherens junction |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761764/ https://www.ncbi.nlm.nih.gov/pubmed/31483697 http://dx.doi.org/10.1091/mbc.E19-04-0216 |
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