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Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast

Although the function of microtubules (MTs) in chromosomal segregation during mitosis is well characterized, much less is known about the role of MTs in chromosomal functions during interphase. In the fission yeast Schizosaccharomyces pombe, dynamic cytoplasmic MT bundles move chromosomes in an osci...

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Autores principales: Zhurinsky, Jacob, Salas-Pino, Silvia, Iglesias-Romero, Ana B., Torres-Mendez, Antonio, Knapp, Benjamin, Flor-Parra, Ignacio, Wang, Jiyong, Bao, Kehan, Jia, Songtao, Chang, Fred, Daga, Rafael R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761766/
https://www.ncbi.nlm.nih.gov/pubmed/31483748
http://dx.doi.org/10.1091/mbc.E19-05-0301
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author Zhurinsky, Jacob
Salas-Pino, Silvia
Iglesias-Romero, Ana B.
Torres-Mendez, Antonio
Knapp, Benjamin
Flor-Parra, Ignacio
Wang, Jiyong
Bao, Kehan
Jia, Songtao
Chang, Fred
Daga, Rafael R.
author_facet Zhurinsky, Jacob
Salas-Pino, Silvia
Iglesias-Romero, Ana B.
Torres-Mendez, Antonio
Knapp, Benjamin
Flor-Parra, Ignacio
Wang, Jiyong
Bao, Kehan
Jia, Songtao
Chang, Fred
Daga, Rafael R.
author_sort Zhurinsky, Jacob
collection PubMed
description Although the function of microtubules (MTs) in chromosomal segregation during mitosis is well characterized, much less is known about the role of MTs in chromosomal functions during interphase. In the fission yeast Schizosaccharomyces pombe, dynamic cytoplasmic MT bundles move chromosomes in an oscillatory manner during interphase via linkages through the nuclear envelope (NE) at the spindle pole body (SPB) and other sites. Mto1 is a cytoplasmic factor that mediates the nucleation and attachment of cytoplasmic MTs to the nucleus. Here, we test the function of these cytoplasmic MTs and Mto1 on DNA repair and recombination during interphase. We find that mto1Δ cells exhibit defects in DNA repair and homologous recombination (HR) and abnormal DNA repair factory dynamics. In these cells, sister chromatids are not properly paired, and binding of Rad21 cohesin subunit along chromosomal arms is reduced. Our findings suggest a model in which cytoplasmic MTs and Mto1 facilitate efficient DNA repair and HR by promoting dynamic chromosomal organization and cohesion in the nucleus.
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spelling pubmed-67617662019-12-16 Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast Zhurinsky, Jacob Salas-Pino, Silvia Iglesias-Romero, Ana B. Torres-Mendez, Antonio Knapp, Benjamin Flor-Parra, Ignacio Wang, Jiyong Bao, Kehan Jia, Songtao Chang, Fred Daga, Rafael R. Mol Biol Cell Articles Although the function of microtubules (MTs) in chromosomal segregation during mitosis is well characterized, much less is known about the role of MTs in chromosomal functions during interphase. In the fission yeast Schizosaccharomyces pombe, dynamic cytoplasmic MT bundles move chromosomes in an oscillatory manner during interphase via linkages through the nuclear envelope (NE) at the spindle pole body (SPB) and other sites. Mto1 is a cytoplasmic factor that mediates the nucleation and attachment of cytoplasmic MTs to the nucleus. Here, we test the function of these cytoplasmic MTs and Mto1 on DNA repair and recombination during interphase. We find that mto1Δ cells exhibit defects in DNA repair and homologous recombination (HR) and abnormal DNA repair factory dynamics. In these cells, sister chromatids are not properly paired, and binding of Rad21 cohesin subunit along chromosomal arms is reduced. Our findings suggest a model in which cytoplasmic MTs and Mto1 facilitate efficient DNA repair and HR by promoting dynamic chromosomal organization and cohesion in the nucleus. The American Society for Cell Biology 2019-10-01 /pmc/articles/PMC6761766/ /pubmed/31483748 http://dx.doi.org/10.1091/mbc.E19-05-0301 Text en © 2019 Zhurinsky, Salas-Pino, Iglesias-Romero, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Zhurinsky, Jacob
Salas-Pino, Silvia
Iglesias-Romero, Ana B.
Torres-Mendez, Antonio
Knapp, Benjamin
Flor-Parra, Ignacio
Wang, Jiyong
Bao, Kehan
Jia, Songtao
Chang, Fred
Daga, Rafael R.
Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast
title Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast
title_full Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast
title_fullStr Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast
title_full_unstemmed Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast
title_short Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast
title_sort effects of the microtubule nucleator mto1 on chromosomal movement, dna repair, and sister chromatid cohesion in fission yeast
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761766/
https://www.ncbi.nlm.nih.gov/pubmed/31483748
http://dx.doi.org/10.1091/mbc.E19-05-0301
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