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Cell therapy stimulates bone neoformation in calvaria defects in rats subjected to local irradiation
BACKGROUND: The purpose of the study was to analyze the effect of cell therapy on the repair process in calvaria defects in rats subjected to irradiation. METHODS: Bone marrow mesenchymal cells were characterized for osteoblastic phenotype. Calvariae of male Wistar rats were irradiated (20 Gy) and,...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6762041/ https://www.ncbi.nlm.nih.gov/pubmed/31773092 http://dx.doi.org/10.1002/ame2.12073 |
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author | Sório, Ana Luisa Riul Vargas‐Sanchez, Paula Katherine Fernandes, Roger Rodrigo Pitol, Dimitrius Leonardo de Sousa, Luiz Gustavo Bianchini, Adriano Luiz Balthazar de Melo, Geraldo Batista Siessere, Selma Bombonato‐Prado, Karina Fittipaldi |
author_facet | Sório, Ana Luisa Riul Vargas‐Sanchez, Paula Katherine Fernandes, Roger Rodrigo Pitol, Dimitrius Leonardo de Sousa, Luiz Gustavo Bianchini, Adriano Luiz Balthazar de Melo, Geraldo Batista Siessere, Selma Bombonato‐Prado, Karina Fittipaldi |
author_sort | Sório, Ana Luisa Riul |
collection | PubMed |
description | BACKGROUND: The purpose of the study was to analyze the effect of cell therapy on the repair process in calvaria defects in rats subjected to irradiation. METHODS: Bone marrow mesenchymal cells were characterized for osteoblastic phenotype. Calvariae of male Wistar rats were irradiated (20 Gy) and, after 4 weeks, osteoblastic cells were placed in surgically created defects in irradiated (IRC) and control animals (CC), paired with untreated irradiated (IR) and control (C) animals. After 30 days, histological and microtomographic evaluation was performed to establish significant (P < 0.05) differences among the groups. RESULTS: Higher alkaline phosphatase detection and activity, along with an increase in mineralized nodules, in the IRC, C and CC groups compared to the IR group, confirmed an osteoblastic phenotype. Histology showed impaired bone neoformation following irradiation, affecting bone marrow composition. Cell therapy in the IRC group improved bone neoformation compared to the IR group. Microtomography revealed increased bone volume, bone surface and trabecular number in IRC group compared to the IR group. CONCLUSION: Cell therapy may improve bone neoformation in defects created after irradiation. |
format | Online Article Text |
id | pubmed-6762041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67620412019-11-26 Cell therapy stimulates bone neoformation in calvaria defects in rats subjected to local irradiation Sório, Ana Luisa Riul Vargas‐Sanchez, Paula Katherine Fernandes, Roger Rodrigo Pitol, Dimitrius Leonardo de Sousa, Luiz Gustavo Bianchini, Adriano Luiz Balthazar de Melo, Geraldo Batista Siessere, Selma Bombonato‐Prado, Karina Fittipaldi Animal Model Exp Med Original Articles BACKGROUND: The purpose of the study was to analyze the effect of cell therapy on the repair process in calvaria defects in rats subjected to irradiation. METHODS: Bone marrow mesenchymal cells were characterized for osteoblastic phenotype. Calvariae of male Wistar rats were irradiated (20 Gy) and, after 4 weeks, osteoblastic cells were placed in surgically created defects in irradiated (IRC) and control animals (CC), paired with untreated irradiated (IR) and control (C) animals. After 30 days, histological and microtomographic evaluation was performed to establish significant (P < 0.05) differences among the groups. RESULTS: Higher alkaline phosphatase detection and activity, along with an increase in mineralized nodules, in the IRC, C and CC groups compared to the IR group, confirmed an osteoblastic phenotype. Histology showed impaired bone neoformation following irradiation, affecting bone marrow composition. Cell therapy in the IRC group improved bone neoformation compared to the IR group. Microtomography revealed increased bone volume, bone surface and trabecular number in IRC group compared to the IR group. CONCLUSION: Cell therapy may improve bone neoformation in defects created after irradiation. John Wiley and Sons Inc. 2019-07-01 /pmc/articles/PMC6762041/ /pubmed/31773092 http://dx.doi.org/10.1002/ame2.12073 Text en © 2019 The Authors. Animal Models and Experimental Medicine published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Sório, Ana Luisa Riul Vargas‐Sanchez, Paula Katherine Fernandes, Roger Rodrigo Pitol, Dimitrius Leonardo de Sousa, Luiz Gustavo Bianchini, Adriano Luiz Balthazar de Melo, Geraldo Batista Siessere, Selma Bombonato‐Prado, Karina Fittipaldi Cell therapy stimulates bone neoformation in calvaria defects in rats subjected to local irradiation |
title | Cell therapy stimulates bone neoformation in calvaria defects in rats subjected to local irradiation |
title_full | Cell therapy stimulates bone neoformation in calvaria defects in rats subjected to local irradiation |
title_fullStr | Cell therapy stimulates bone neoformation in calvaria defects in rats subjected to local irradiation |
title_full_unstemmed | Cell therapy stimulates bone neoformation in calvaria defects in rats subjected to local irradiation |
title_short | Cell therapy stimulates bone neoformation in calvaria defects in rats subjected to local irradiation |
title_sort | cell therapy stimulates bone neoformation in calvaria defects in rats subjected to local irradiation |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6762041/ https://www.ncbi.nlm.nih.gov/pubmed/31773092 http://dx.doi.org/10.1002/ame2.12073 |
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