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Distribution of SET/I2PP2A protein in gastrointestinal tissues

SET (also called I2PP2A and TIF-1) is a multi-functional protein that regulates a variety of cell signaling including nucleosome assembly, histone binding, and tumorigenesis. Elevated SET protein levels are observed in various human tumors, and are correlated with poor prognosis and drug-resistance....

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Autores principales: Umata, Koji, Sakai, Yusuke, Ikeda, Shunta, Tsuji, Shunya, Kawasaki, Hideyoshi, Ohama, Takashi, Sato, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6762106/
https://www.ncbi.nlm.nih.gov/pubmed/31557212
http://dx.doi.org/10.1371/journal.pone.0222845
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author Umata, Koji
Sakai, Yusuke
Ikeda, Shunta
Tsuji, Shunya
Kawasaki, Hideyoshi
Ohama, Takashi
Sato, Koichi
author_facet Umata, Koji
Sakai, Yusuke
Ikeda, Shunta
Tsuji, Shunya
Kawasaki, Hideyoshi
Ohama, Takashi
Sato, Koichi
author_sort Umata, Koji
collection PubMed
description SET (also called I2PP2A and TIF-1) is a multi-functional protein that regulates a variety of cell signaling including nucleosome assembly, histone binding, and tumorigenesis. Elevated SET protein levels are observed in various human tumors, and are correlated with poor prognosis and drug-resistance. We recently reported that SET protein levels in cancer cells were positively correlated with poor prognosis of gastric cancer patients. Using immunohistochemistry, SET protein was observed not only in cancer cells, but also in some interstitial cells. However, the tissue distribution of SET has not been investigated. Here we performed co-immunofluorescent staining to characterize SET protein distribution in gastrointestinal tissues. We found that even though the positive rate is much lower than epithelial cells, SET protein is also expressed in non-epithelial cells, such as monocytes/macrophages, neural cells, myofibroblasts, and smooth muscle cells. Our results indicate an extensive role of SET in a variety of cell types.
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spelling pubmed-67621062019-10-12 Distribution of SET/I2PP2A protein in gastrointestinal tissues Umata, Koji Sakai, Yusuke Ikeda, Shunta Tsuji, Shunya Kawasaki, Hideyoshi Ohama, Takashi Sato, Koichi PLoS One Research Article SET (also called I2PP2A and TIF-1) is a multi-functional protein that regulates a variety of cell signaling including nucleosome assembly, histone binding, and tumorigenesis. Elevated SET protein levels are observed in various human tumors, and are correlated with poor prognosis and drug-resistance. We recently reported that SET protein levels in cancer cells were positively correlated with poor prognosis of gastric cancer patients. Using immunohistochemistry, SET protein was observed not only in cancer cells, but also in some interstitial cells. However, the tissue distribution of SET has not been investigated. Here we performed co-immunofluorescent staining to characterize SET protein distribution in gastrointestinal tissues. We found that even though the positive rate is much lower than epithelial cells, SET protein is also expressed in non-epithelial cells, such as monocytes/macrophages, neural cells, myofibroblasts, and smooth muscle cells. Our results indicate an extensive role of SET in a variety of cell types. Public Library of Science 2019-09-26 /pmc/articles/PMC6762106/ /pubmed/31557212 http://dx.doi.org/10.1371/journal.pone.0222845 Text en © 2019 Umata et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Umata, Koji
Sakai, Yusuke
Ikeda, Shunta
Tsuji, Shunya
Kawasaki, Hideyoshi
Ohama, Takashi
Sato, Koichi
Distribution of SET/I2PP2A protein in gastrointestinal tissues
title Distribution of SET/I2PP2A protein in gastrointestinal tissues
title_full Distribution of SET/I2PP2A protein in gastrointestinal tissues
title_fullStr Distribution of SET/I2PP2A protein in gastrointestinal tissues
title_full_unstemmed Distribution of SET/I2PP2A protein in gastrointestinal tissues
title_short Distribution of SET/I2PP2A protein in gastrointestinal tissues
title_sort distribution of set/i2pp2a protein in gastrointestinal tissues
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6762106/
https://www.ncbi.nlm.nih.gov/pubmed/31557212
http://dx.doi.org/10.1371/journal.pone.0222845
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