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Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet

We hypothesized that postnatal development of the vagina is impacted by early nutritional environment. Our objective was to determine if lipid profiles of vaginal swabs were different between postnatal gilts suckled by sow or fed milk replacer the first 48 h after birth, with or without a lard-based...

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Autores principales: Harlow, KaLynn, Ferreira, Christina R., Sobreira, Tiago J. P., Casey, Theresa, Stewart, Kara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6762109/
https://www.ncbi.nlm.nih.gov/pubmed/31557164
http://dx.doi.org/10.1371/journal.pone.0215186
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author Harlow, KaLynn
Ferreira, Christina R.
Sobreira, Tiago J. P.
Casey, Theresa
Stewart, Kara
author_facet Harlow, KaLynn
Ferreira, Christina R.
Sobreira, Tiago J. P.
Casey, Theresa
Stewart, Kara
author_sort Harlow, KaLynn
collection PubMed
description We hypothesized that postnatal development of the vagina is impacted by early nutritional environment. Our objective was to determine if lipid profiles of vaginal swabs were different between postnatal gilts suckled by sow or fed milk replacer the first 48 h after birth, with or without a lard-based fat supplement. Gilts (>1.3 kg) were selected at birth across 8 litters and assigned to one of four treatments: 1) suckled by sow (S, n = 8); 2) suckled by sow plus administration of a fat supplement (SF, n = 5); 3) bottle-fed solely milk replacer (B, n = 8); or 4) bottle-fed solely milk replacer plus administration of a fat supplement (BF, n = 7). At 48 h postnatal, vaginal swabs of gilts were taken with a cytology brush, and lipids were extracted for analysis using multiple reaction monitoring (MRM)-profiling. Lipids extracted from serum collected at 48 h from gilts, milk collected at 24 h from sows, and milk replacer were also analyzed with MRM-profiling. Receiver operating characteristic curve analysis found 18 lipids recovered from vaginal swabs that highly distinguished between S and B gilts [area-under-the-curve (AUC) > 0.9], including phosphatidylethanolamine with 34 carbons and four unsaturations in the fatty acyl residues [PE (34:4)]. Twelve lipids from vaginal swabs highly correlated (r > 0.6; p < 0.01) with nutrition source. Lipids with greater abundance in milk replacer drove association. For example, mean intensity of PE (34:4) was 149-fold higher in milk replacer than colostrum. Consequently, PE (34:4) was found to have 1.6- and 2.12-fold higher levels in serum and vaginal swab samples (p < 0.001), respectively, of B gilts as compared to S gilts. Findings support that vaginal swabs can be used to noninvasively study effects of perinatal nutrition on tissue composition.
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spelling pubmed-67621092019-10-12 Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet Harlow, KaLynn Ferreira, Christina R. Sobreira, Tiago J. P. Casey, Theresa Stewart, Kara PLoS One Research Article We hypothesized that postnatal development of the vagina is impacted by early nutritional environment. Our objective was to determine if lipid profiles of vaginal swabs were different between postnatal gilts suckled by sow or fed milk replacer the first 48 h after birth, with or without a lard-based fat supplement. Gilts (>1.3 kg) were selected at birth across 8 litters and assigned to one of four treatments: 1) suckled by sow (S, n = 8); 2) suckled by sow plus administration of a fat supplement (SF, n = 5); 3) bottle-fed solely milk replacer (B, n = 8); or 4) bottle-fed solely milk replacer plus administration of a fat supplement (BF, n = 7). At 48 h postnatal, vaginal swabs of gilts were taken with a cytology brush, and lipids were extracted for analysis using multiple reaction monitoring (MRM)-profiling. Lipids extracted from serum collected at 48 h from gilts, milk collected at 24 h from sows, and milk replacer were also analyzed with MRM-profiling. Receiver operating characteristic curve analysis found 18 lipids recovered from vaginal swabs that highly distinguished between S and B gilts [area-under-the-curve (AUC) > 0.9], including phosphatidylethanolamine with 34 carbons and four unsaturations in the fatty acyl residues [PE (34:4)]. Twelve lipids from vaginal swabs highly correlated (r > 0.6; p < 0.01) with nutrition source. Lipids with greater abundance in milk replacer drove association. For example, mean intensity of PE (34:4) was 149-fold higher in milk replacer than colostrum. Consequently, PE (34:4) was found to have 1.6- and 2.12-fold higher levels in serum and vaginal swab samples (p < 0.001), respectively, of B gilts as compared to S gilts. Findings support that vaginal swabs can be used to noninvasively study effects of perinatal nutrition on tissue composition. Public Library of Science 2019-09-26 /pmc/articles/PMC6762109/ /pubmed/31557164 http://dx.doi.org/10.1371/journal.pone.0215186 Text en © 2019 Harlow et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Harlow, KaLynn
Ferreira, Christina R.
Sobreira, Tiago J. P.
Casey, Theresa
Stewart, Kara
Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet
title Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet
title_full Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet
title_fullStr Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet
title_full_unstemmed Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet
title_short Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet
title_sort lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6762109/
https://www.ncbi.nlm.nih.gov/pubmed/31557164
http://dx.doi.org/10.1371/journal.pone.0215186
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