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Genetic analysis and fine mapping of a qualitative trait locus wpb1 for albino panicle branches in rice
Chloroplast plays an important role in the plant life cycle. However, the details of its development remain elusive in rice. In this study, we report the fine-mapping of a novel rice gene wpb1 (white panicle branch 1), which affects chloroplast biogenesis, from a tropical japonica variety that resul...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763196/ https://www.ncbi.nlm.nih.gov/pubmed/31557269 http://dx.doi.org/10.1371/journal.pone.0223228 |
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author | Cai, Zhongquan Jia, Peilong Zhang, Jiaqiang Gan, Ping Shao, Qi Jin, Gang Wang, Liping Jin, Jian Yang, Jiangyi Luo, Jijing |
author_facet | Cai, Zhongquan Jia, Peilong Zhang, Jiaqiang Gan, Ping Shao, Qi Jin, Gang Wang, Liping Jin, Jian Yang, Jiangyi Luo, Jijing |
author_sort | Cai, Zhongquan |
collection | PubMed |
description | Chloroplast plays an important role in the plant life cycle. However, the details of its development remain elusive in rice. In this study, we report the fine-mapping of a novel rice gene wpb1 (white panicle branch 1), which affects chloroplast biogenesis, from a tropical japonica variety that results in an albino panicle branches at and after the heading stage. The wpb1 variety was crossed with Nipponbare to generate the F(2) and BC(1)F(2) populations. Green and white panicle branch phenotypes with a 3:1 segregation ratio was observed in the F(2) population. Bulked segregant analysis (BSA) based on whole genome resequencing was conducted to determine the wpb1 locus. A candidate interval spanning from 11.35 to 23.79M (physical position) on chromosome 1 was identified. The results of BSA analysis were verified by a 40K rice SNP-array using the BC(1)F(2) population. A large-scale F(2) population was used to pinpoint wpb1, and the locus was further narrowed down to a 95-kb interval. Furthermore, our results showed that the expression levels of the majority of the genes involved in Chl biosynthesis, photosynthesis and chloroplast development were remarkably affected in wpb1 variety and in F(2) plants with a white panicle branch phenotype. In line with the results mentioned above, anatomical structural examination and chlorophyll (Chl) content measurement suggested that wpb1 might play an important role in the regulation of chloroplast development. Further cloning and functional characterization of the wpb1 gene will shed light on the molecular mechanism underlying chloroplast development in rice. |
format | Online Article Text |
id | pubmed-6763196 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-67631962019-10-12 Genetic analysis and fine mapping of a qualitative trait locus wpb1 for albino panicle branches in rice Cai, Zhongquan Jia, Peilong Zhang, Jiaqiang Gan, Ping Shao, Qi Jin, Gang Wang, Liping Jin, Jian Yang, Jiangyi Luo, Jijing PLoS One Research Article Chloroplast plays an important role in the plant life cycle. However, the details of its development remain elusive in rice. In this study, we report the fine-mapping of a novel rice gene wpb1 (white panicle branch 1), which affects chloroplast biogenesis, from a tropical japonica variety that results in an albino panicle branches at and after the heading stage. The wpb1 variety was crossed with Nipponbare to generate the F(2) and BC(1)F(2) populations. Green and white panicle branch phenotypes with a 3:1 segregation ratio was observed in the F(2) population. Bulked segregant analysis (BSA) based on whole genome resequencing was conducted to determine the wpb1 locus. A candidate interval spanning from 11.35 to 23.79M (physical position) on chromosome 1 was identified. The results of BSA analysis were verified by a 40K rice SNP-array using the BC(1)F(2) population. A large-scale F(2) population was used to pinpoint wpb1, and the locus was further narrowed down to a 95-kb interval. Furthermore, our results showed that the expression levels of the majority of the genes involved in Chl biosynthesis, photosynthesis and chloroplast development were remarkably affected in wpb1 variety and in F(2) plants with a white panicle branch phenotype. In line with the results mentioned above, anatomical structural examination and chlorophyll (Chl) content measurement suggested that wpb1 might play an important role in the regulation of chloroplast development. Further cloning and functional characterization of the wpb1 gene will shed light on the molecular mechanism underlying chloroplast development in rice. Public Library of Science 2019-09-26 /pmc/articles/PMC6763196/ /pubmed/31557269 http://dx.doi.org/10.1371/journal.pone.0223228 Text en © 2019 Cai et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Cai, Zhongquan Jia, Peilong Zhang, Jiaqiang Gan, Ping Shao, Qi Jin, Gang Wang, Liping Jin, Jian Yang, Jiangyi Luo, Jijing Genetic analysis and fine mapping of a qualitative trait locus wpb1 for albino panicle branches in rice |
title | Genetic analysis and fine mapping of a qualitative trait locus wpb1 for albino panicle branches in rice |
title_full | Genetic analysis and fine mapping of a qualitative trait locus wpb1 for albino panicle branches in rice |
title_fullStr | Genetic analysis and fine mapping of a qualitative trait locus wpb1 for albino panicle branches in rice |
title_full_unstemmed | Genetic analysis and fine mapping of a qualitative trait locus wpb1 for albino panicle branches in rice |
title_short | Genetic analysis and fine mapping of a qualitative trait locus wpb1 for albino panicle branches in rice |
title_sort | genetic analysis and fine mapping of a qualitative trait locus wpb1 for albino panicle branches in rice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763196/ https://www.ncbi.nlm.nih.gov/pubmed/31557269 http://dx.doi.org/10.1371/journal.pone.0223228 |
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