Cargando…
Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display
Self-assembling virus-like particles represent highly attractive tools for developing next-generation vaccines and protein therapeutics. We created ADDomer, an adenovirus-derived multimeric protein-based self-assembling nanoparticle scaffold engineered to facilitate plug-and-play display of multiple...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763337/ https://www.ncbi.nlm.nih.gov/pubmed/31620562 http://dx.doi.org/10.1126/sciadv.aaw2853 |
_version_ | 1783454184470740992 |
---|---|
author | Vragniau, Charles Bufton, Joshua C. Garzoni, Frédéric Stermann, Emilie Rabi, Fruzsina Terrat, Céline Guidetti, Mélanie Josserand, Véronique Williams, Matt Woods, Christopher J. Viedma, Gerardo Bates, Phil Verrier, Bernard Chaperot, Laurence Schaffitzel, Christiane Berger, Imre Fender, Pascal |
author_facet | Vragniau, Charles Bufton, Joshua C. Garzoni, Frédéric Stermann, Emilie Rabi, Fruzsina Terrat, Céline Guidetti, Mélanie Josserand, Véronique Williams, Matt Woods, Christopher J. Viedma, Gerardo Bates, Phil Verrier, Bernard Chaperot, Laurence Schaffitzel, Christiane Berger, Imre Fender, Pascal |
author_sort | Vragniau, Charles |
collection | PubMed |
description | Self-assembling virus-like particles represent highly attractive tools for developing next-generation vaccines and protein therapeutics. We created ADDomer, an adenovirus-derived multimeric protein-based self-assembling nanoparticle scaffold engineered to facilitate plug-and-play display of multiple immunogenic epitopes from pathogens. We used cryo–electron microscopy at near-atomic resolution and implemented novel, cost-effective, high-performance cloud computing to reveal architectural features in unprecedented detail. We analyzed ADDomer interaction with components of the immune system and developed a promising first-in-kind ADDomer-based vaccine candidate to combat emerging Chikungunya infectious disease, exemplifying the potential of our approach. |
format | Online Article Text |
id | pubmed-6763337 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-67633372019-10-16 Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display Vragniau, Charles Bufton, Joshua C. Garzoni, Frédéric Stermann, Emilie Rabi, Fruzsina Terrat, Céline Guidetti, Mélanie Josserand, Véronique Williams, Matt Woods, Christopher J. Viedma, Gerardo Bates, Phil Verrier, Bernard Chaperot, Laurence Schaffitzel, Christiane Berger, Imre Fender, Pascal Sci Adv Research Articles Self-assembling virus-like particles represent highly attractive tools for developing next-generation vaccines and protein therapeutics. We created ADDomer, an adenovirus-derived multimeric protein-based self-assembling nanoparticle scaffold engineered to facilitate plug-and-play display of multiple immunogenic epitopes from pathogens. We used cryo–electron microscopy at near-atomic resolution and implemented novel, cost-effective, high-performance cloud computing to reveal architectural features in unprecedented detail. We analyzed ADDomer interaction with components of the immune system and developed a promising first-in-kind ADDomer-based vaccine candidate to combat emerging Chikungunya infectious disease, exemplifying the potential of our approach. American Association for the Advancement of Science 2019-09-25 /pmc/articles/PMC6763337/ /pubmed/31620562 http://dx.doi.org/10.1126/sciadv.aaw2853 Text en Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Vragniau, Charles Bufton, Joshua C. Garzoni, Frédéric Stermann, Emilie Rabi, Fruzsina Terrat, Céline Guidetti, Mélanie Josserand, Véronique Williams, Matt Woods, Christopher J. Viedma, Gerardo Bates, Phil Verrier, Bernard Chaperot, Laurence Schaffitzel, Christiane Berger, Imre Fender, Pascal Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display |
title | Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display |
title_full | Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display |
title_fullStr | Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display |
title_full_unstemmed | Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display |
title_short | Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display |
title_sort | synthetic self-assembling addomer platform for highly efficient vaccination by genetically encoded multiepitope display |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763337/ https://www.ncbi.nlm.nih.gov/pubmed/31620562 http://dx.doi.org/10.1126/sciadv.aaw2853 |
work_keys_str_mv | AT vragniaucharles syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT buftonjoshuac syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT garzonifrederic syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT stermannemilie syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT rabifruzsina syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT terratceline syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT guidettimelanie syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT josserandveronique syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT williamsmatt syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT woodschristopherj syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT viedmagerardo syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT batesphil syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT verrierbernard syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT chaperotlaurence syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT schaffitzelchristiane syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT bergerimre syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay AT fenderpascal syntheticselfassemblingaddomerplatformforhighlyefficientvaccinationbygeneticallyencodedmultiepitopedisplay |