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Osmolyte homeostasis controls single-cell growth rate and maximum cell size of Saccharomyces cerevisiae
Cell growth is well described at the population level, but precisely how nutrient and water uptake and cell wall expansion drive the growth of single cells is poorly understood. Supported by measurements of single-cell growth trajectories and cell wall elasticity, we present a single-cell growth mod...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763471/ https://www.ncbi.nlm.nih.gov/pubmed/31583116 http://dx.doi.org/10.1038/s41540-019-0111-6 |
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author | Altenburg, Tom Goldenbogen, Björn Uhlendorf, Jannis Klipp, Edda |
author_facet | Altenburg, Tom Goldenbogen, Björn Uhlendorf, Jannis Klipp, Edda |
author_sort | Altenburg, Tom |
collection | PubMed |
description | Cell growth is well described at the population level, but precisely how nutrient and water uptake and cell wall expansion drive the growth of single cells is poorly understood. Supported by measurements of single-cell growth trajectories and cell wall elasticity, we present a single-cell growth model for yeast. The model links the thermodynamic quantities, such as turgor pressure, osmolarity, cell wall elasto-plasticity, and cell size, applying concepts from rheology and thin shell theory. It reproduces cell size dynamics during single-cell growth, budding, and hyper-osmotic or hypo-osmotic stress. We find that single-cell growth rate and final size are primarily governed by osmolyte uptake and consumption, while bud expansion requires additionally different cell wall extensibilities between mother and bud. Based on first principles the model provides a more accurate description of size dynamics than previous attempts and its analytical simplification allows for easy combination with models for other cell processes. |
format | Online Article Text |
id | pubmed-6763471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67634712019-10-03 Osmolyte homeostasis controls single-cell growth rate and maximum cell size of Saccharomyces cerevisiae Altenburg, Tom Goldenbogen, Björn Uhlendorf, Jannis Klipp, Edda NPJ Syst Biol Appl Article Cell growth is well described at the population level, but precisely how nutrient and water uptake and cell wall expansion drive the growth of single cells is poorly understood. Supported by measurements of single-cell growth trajectories and cell wall elasticity, we present a single-cell growth model for yeast. The model links the thermodynamic quantities, such as turgor pressure, osmolarity, cell wall elasto-plasticity, and cell size, applying concepts from rheology and thin shell theory. It reproduces cell size dynamics during single-cell growth, budding, and hyper-osmotic or hypo-osmotic stress. We find that single-cell growth rate and final size are primarily governed by osmolyte uptake and consumption, while bud expansion requires additionally different cell wall extensibilities between mother and bud. Based on first principles the model provides a more accurate description of size dynamics than previous attempts and its analytical simplification allows for easy combination with models for other cell processes. Nature Publishing Group UK 2019-09-26 /pmc/articles/PMC6763471/ /pubmed/31583116 http://dx.doi.org/10.1038/s41540-019-0111-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Altenburg, Tom Goldenbogen, Björn Uhlendorf, Jannis Klipp, Edda Osmolyte homeostasis controls single-cell growth rate and maximum cell size of Saccharomyces cerevisiae |
title | Osmolyte homeostasis controls single-cell growth rate and maximum cell size of Saccharomyces cerevisiae |
title_full | Osmolyte homeostasis controls single-cell growth rate and maximum cell size of Saccharomyces cerevisiae |
title_fullStr | Osmolyte homeostasis controls single-cell growth rate and maximum cell size of Saccharomyces cerevisiae |
title_full_unstemmed | Osmolyte homeostasis controls single-cell growth rate and maximum cell size of Saccharomyces cerevisiae |
title_short | Osmolyte homeostasis controls single-cell growth rate and maximum cell size of Saccharomyces cerevisiae |
title_sort | osmolyte homeostasis controls single-cell growth rate and maximum cell size of saccharomyces cerevisiae |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763471/ https://www.ncbi.nlm.nih.gov/pubmed/31583116 http://dx.doi.org/10.1038/s41540-019-0111-6 |
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