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Protein quantification and visualization via ultraviolet-dependent labeling with 2,2,2-trichloroethanol

The incorporation of 2,2,2-trichloroethanol in polyacrylamide gels allows for fluorescent visualization of proteins following electrophoresis. Ultraviolet-light exposure, in the presence of this trichlorinated compound, results in a covalent modification of the tryptophan indole ring that shifts the...

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Detalles Bibliográficos
Autores principales: Chopra, Anand, Willmore, William G., Biggar, Kyle K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763483/
https://www.ncbi.nlm.nih.gov/pubmed/31558752
http://dx.doi.org/10.1038/s41598-019-50385-9