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Protein quantification and visualization via ultraviolet-dependent labeling with 2,2,2-trichloroethanol
The incorporation of 2,2,2-trichloroethanol in polyacrylamide gels allows for fluorescent visualization of proteins following electrophoresis. Ultraviolet-light exposure, in the presence of this trichlorinated compound, results in a covalent modification of the tryptophan indole ring that shifts the...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763483/ https://www.ncbi.nlm.nih.gov/pubmed/31558752 http://dx.doi.org/10.1038/s41598-019-50385-9 |