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Distinct transcriptional roles for Histone H3-K56 acetylation during the cell cycle in Yeast

Dynamic disruption and reassembly of promoter-proximal nucleosomes is a conserved hallmark of transcriptionally active chromatin. Histone H3-K56 acetylation (H3K56Ac) enhances these turnover events and promotes nucleosome assembly during S phase. Here we sequence nascent transcripts to investigate t...

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Autores principales: Topal, Salih, Vasseur, Pauline, Radman-Livaja, Marta, Peterson, Craig L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763489/
https://www.ncbi.nlm.nih.gov/pubmed/31558720
http://dx.doi.org/10.1038/s41467-019-12400-5
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author Topal, Salih
Vasseur, Pauline
Radman-Livaja, Marta
Peterson, Craig L.
author_facet Topal, Salih
Vasseur, Pauline
Radman-Livaja, Marta
Peterson, Craig L.
author_sort Topal, Salih
collection PubMed
description Dynamic disruption and reassembly of promoter-proximal nucleosomes is a conserved hallmark of transcriptionally active chromatin. Histone H3-K56 acetylation (H3K56Ac) enhances these turnover events and promotes nucleosome assembly during S phase. Here we sequence nascent transcripts to investigate the impact of H3K56Ac on transcription throughout the yeast cell cycle. We find that H3K56Ac is a genome-wide activator of transcription. While H3K56Ac has a major impact on transcription initiation, it also appears to promote elongation and/or termination. In contrast, H3K56Ac represses promiscuous transcription that occurs immediately following replication fork passage, in this case by promoting efficient nucleosome assembly. We also detect a stepwise increase in transcription as cells transit S phase and enter G2, but this response to increased gene dosage does not require H3K56Ac. Thus, a single histone mark can exert both positive and negative impacts on transcription that are coupled to different cell cycle events.
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spelling pubmed-67634892019-09-30 Distinct transcriptional roles for Histone H3-K56 acetylation during the cell cycle in Yeast Topal, Salih Vasseur, Pauline Radman-Livaja, Marta Peterson, Craig L. Nat Commun Article Dynamic disruption and reassembly of promoter-proximal nucleosomes is a conserved hallmark of transcriptionally active chromatin. Histone H3-K56 acetylation (H3K56Ac) enhances these turnover events and promotes nucleosome assembly during S phase. Here we sequence nascent transcripts to investigate the impact of H3K56Ac on transcription throughout the yeast cell cycle. We find that H3K56Ac is a genome-wide activator of transcription. While H3K56Ac has a major impact on transcription initiation, it also appears to promote elongation and/or termination. In contrast, H3K56Ac represses promiscuous transcription that occurs immediately following replication fork passage, in this case by promoting efficient nucleosome assembly. We also detect a stepwise increase in transcription as cells transit S phase and enter G2, but this response to increased gene dosage does not require H3K56Ac. Thus, a single histone mark can exert both positive and negative impacts on transcription that are coupled to different cell cycle events. Nature Publishing Group UK 2019-09-26 /pmc/articles/PMC6763489/ /pubmed/31558720 http://dx.doi.org/10.1038/s41467-019-12400-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Topal, Salih
Vasseur, Pauline
Radman-Livaja, Marta
Peterson, Craig L.
Distinct transcriptional roles for Histone H3-K56 acetylation during the cell cycle in Yeast
title Distinct transcriptional roles for Histone H3-K56 acetylation during the cell cycle in Yeast
title_full Distinct transcriptional roles for Histone H3-K56 acetylation during the cell cycle in Yeast
title_fullStr Distinct transcriptional roles for Histone H3-K56 acetylation during the cell cycle in Yeast
title_full_unstemmed Distinct transcriptional roles for Histone H3-K56 acetylation during the cell cycle in Yeast
title_short Distinct transcriptional roles for Histone H3-K56 acetylation during the cell cycle in Yeast
title_sort distinct transcriptional roles for histone h3-k56 acetylation during the cell cycle in yeast
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763489/
https://www.ncbi.nlm.nih.gov/pubmed/31558720
http://dx.doi.org/10.1038/s41467-019-12400-5
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