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Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis
Diagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing (NGS) is an emerging approach that allows pathogen identification in challenging situations, as evi...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763761/ https://www.ncbi.nlm.nih.gov/pubmed/31616669 http://dx.doi.org/10.3389/fmed.2019.00203 |
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author | Kolb, Manon Lazarevic, Vladimir Emonet, Stéphane Calmy, Alexandra Girard, Myriam Gaïa, Nadia Charretier, Yannick Cherkaoui, Abdessalam Keller, Peter Huber, Christoph Schrenzel, Jacques |
author_facet | Kolb, Manon Lazarevic, Vladimir Emonet, Stéphane Calmy, Alexandra Girard, Myriam Gaïa, Nadia Charretier, Yannick Cherkaoui, Abdessalam Keller, Peter Huber, Christoph Schrenzel, Jacques |
author_sort | Kolb, Manon |
collection | PubMed |
description | Diagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing (NGS) is an emerging approach that allows pathogen identification in challenging situations, as evidenced by a clinical case. We sequenced the DNA extracted from the surgically-removed frozen valve tissue from a patient with suspected infective endocarditis with negative blood and valve cultures. Mapping of the sequence reads against reference genomic sequences, a 16S rRNA gene database and clade-specific marker genes suggested an infection caused by Cardiobacterium hominis. |
format | Online Article Text |
id | pubmed-6763761 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67637612019-10-15 Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis Kolb, Manon Lazarevic, Vladimir Emonet, Stéphane Calmy, Alexandra Girard, Myriam Gaïa, Nadia Charretier, Yannick Cherkaoui, Abdessalam Keller, Peter Huber, Christoph Schrenzel, Jacques Front Med (Lausanne) Medicine Diagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing (NGS) is an emerging approach that allows pathogen identification in challenging situations, as evidenced by a clinical case. We sequenced the DNA extracted from the surgically-removed frozen valve tissue from a patient with suspected infective endocarditis with negative blood and valve cultures. Mapping of the sequence reads against reference genomic sequences, a 16S rRNA gene database and clade-specific marker genes suggested an infection caused by Cardiobacterium hominis. Frontiers Media S.A. 2019-09-20 /pmc/articles/PMC6763761/ /pubmed/31616669 http://dx.doi.org/10.3389/fmed.2019.00203 Text en Copyright © 2019 Kolb, Lazarevic, Emonet, Calmy, Girard, Gaïa, Charretier, Cherkaoui, Keller, Huber and Schrenzel. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medicine Kolb, Manon Lazarevic, Vladimir Emonet, Stéphane Calmy, Alexandra Girard, Myriam Gaïa, Nadia Charretier, Yannick Cherkaoui, Abdessalam Keller, Peter Huber, Christoph Schrenzel, Jacques Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis |
title | Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis |
title_full | Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis |
title_fullStr | Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis |
title_full_unstemmed | Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis |
title_short | Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis |
title_sort | next-generation sequencing for the diagnosis of challenging culture-negative endocarditis |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6763761/ https://www.ncbi.nlm.nih.gov/pubmed/31616669 http://dx.doi.org/10.3389/fmed.2019.00203 |
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