Influence of time and phenotype on salivary Fel d1 in domestic shorthair cats

OBJECTIVES: Fel d1 is a major allergen that may affect humans sensitive to cat allergens, and it can be detected in the saliva and on the hair of cats. We studied the variability of salivary Fel d1 in typical house cats (ie, neutered domestic shorthair cats) and the factors that could be associated with that variability. METHODS: Saliva samples were collected from 64 cats, twice daily, every other day, for a year, at two locations (Missouri, USA, and Ontario, Canada). Salivary Fel d1 levels were measured using an immunoassay. Correlations and linear mixed-effects model analyses were run to assess which factors significantly affected the Fel d1 levels. RESULTS: Salivary Fel d1 levels varied significantly both within and among cats. Cat averages over the year ranged from 0.4–35 µg/ml, and a higher average correlated with a higher SD (P <0.001). The first collection of the day tended to be higher than the afternoon collection (P <0.001). Sex, coat color or body size did not relate to cats’ average Fel d1 production, but older cats tended to have lower salivary Fel d1 levels (P <0.001). Fel d1 levels from four samples were reliable in identifying cats producing stable low levels of Fel d1. CONCLUSIONS AND RELEVANCE: We observed a wide and continuous range of salivary Fel d1 production in domestic shorthair cats. In particular, a subset of cats had stable low levels throughout the course of the year, and they can be identified by analyzing a few saliva samples rather than their physical appearance.