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FgPex3, a Peroxisome Biogenesis Factor, Is Involved in Regulating Vegetative Growth, Conidiation, Sexual Development, and Virulence in Fusarium graminearum

Peroxisomes are involved in a wide range of important cellular functions. Here, the role of the peroxisomal membrane protein PEX3 in the plant-pathogen and mycotoxin producer Fusarium graminearum was studied using knock-out and complemented strains. To fluorescently label peroxisomes’ punctate struc...

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Autores principales: Kong, Xiangjiu, Zhang, Hao, Wang, Xiaoliang, van der Lee, Theo, Waalwijk, Cees, van Diepeningen, Anne, Brankovics, Balazs, Xu, Jin, Xu, Jingsheng, Chen, Wanquan, Feng, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764106/
https://www.ncbi.nlm.nih.gov/pubmed/31616386
http://dx.doi.org/10.3389/fmicb.2019.02088
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author Kong, Xiangjiu
Zhang, Hao
Wang, Xiaoliang
van der Lee, Theo
Waalwijk, Cees
van Diepeningen, Anne
Brankovics, Balazs
Xu, Jin
Xu, Jingsheng
Chen, Wanquan
Feng, Jie
author_facet Kong, Xiangjiu
Zhang, Hao
Wang, Xiaoliang
van der Lee, Theo
Waalwijk, Cees
van Diepeningen, Anne
Brankovics, Balazs
Xu, Jin
Xu, Jingsheng
Chen, Wanquan
Feng, Jie
author_sort Kong, Xiangjiu
collection PubMed
description Peroxisomes are involved in a wide range of important cellular functions. Here, the role of the peroxisomal membrane protein PEX3 in the plant-pathogen and mycotoxin producer Fusarium graminearum was studied using knock-out and complemented strains. To fluorescently label peroxisomes’ punctate structures, GFP and RFP fusions with the PTS1 and PTS2 localization signal were transformed into the wild type PH-1 and ΔFgPex3 knock-out strains. The GFP and RFP transformants in the ΔFgPex3 background showed a diffuse fluorescence pattern across the cytoplasm suggesting the absence of mature peroxisomes. The ΔFgPex3 strain showed a minor, non-significant reduction in growth on various sugar carbon sources. In contrast, deletion of FgPex3 affected fatty acid β-oxidation in F. graminearum and significantly reduced the utilization of fatty acids. Furthermore, the ΔFgPex3 mutant was sensitive to osmotic stressors as well as to cell wall-damaging agents. Reactive oxygen species (ROS) levels in the mutant had increased significantly, which may be linked to the reduced longevity of cultured strains. The mutant also showed reduced production of conidiospores, while sexual reproduction was completely impaired. The pathogenicity of ΔFgPex3, especially during the process of systemic infection, was strongly reduced on both tomato and on wheat, while to production of deoxynivalenol (DON), an important factor for virulence, appeared to be unaffected.
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spelling pubmed-67641062019-10-15 FgPex3, a Peroxisome Biogenesis Factor, Is Involved in Regulating Vegetative Growth, Conidiation, Sexual Development, and Virulence in Fusarium graminearum Kong, Xiangjiu Zhang, Hao Wang, Xiaoliang van der Lee, Theo Waalwijk, Cees van Diepeningen, Anne Brankovics, Balazs Xu, Jin Xu, Jingsheng Chen, Wanquan Feng, Jie Front Microbiol Microbiology Peroxisomes are involved in a wide range of important cellular functions. Here, the role of the peroxisomal membrane protein PEX3 in the plant-pathogen and mycotoxin producer Fusarium graminearum was studied using knock-out and complemented strains. To fluorescently label peroxisomes’ punctate structures, GFP and RFP fusions with the PTS1 and PTS2 localization signal were transformed into the wild type PH-1 and ΔFgPex3 knock-out strains. The GFP and RFP transformants in the ΔFgPex3 background showed a diffuse fluorescence pattern across the cytoplasm suggesting the absence of mature peroxisomes. The ΔFgPex3 strain showed a minor, non-significant reduction in growth on various sugar carbon sources. In contrast, deletion of FgPex3 affected fatty acid β-oxidation in F. graminearum and significantly reduced the utilization of fatty acids. Furthermore, the ΔFgPex3 mutant was sensitive to osmotic stressors as well as to cell wall-damaging agents. Reactive oxygen species (ROS) levels in the mutant had increased significantly, which may be linked to the reduced longevity of cultured strains. The mutant also showed reduced production of conidiospores, while sexual reproduction was completely impaired. The pathogenicity of ΔFgPex3, especially during the process of systemic infection, was strongly reduced on both tomato and on wheat, while to production of deoxynivalenol (DON), an important factor for virulence, appeared to be unaffected. Frontiers Media S.A. 2019-09-20 /pmc/articles/PMC6764106/ /pubmed/31616386 http://dx.doi.org/10.3389/fmicb.2019.02088 Text en Copyright © 2019 Kong, Zhang, Wang, van der Lee, Waalwijk, van Diepeningen, Brankovics, Xu, Xu, Chen and Feng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Kong, Xiangjiu
Zhang, Hao
Wang, Xiaoliang
van der Lee, Theo
Waalwijk, Cees
van Diepeningen, Anne
Brankovics, Balazs
Xu, Jin
Xu, Jingsheng
Chen, Wanquan
Feng, Jie
FgPex3, a Peroxisome Biogenesis Factor, Is Involved in Regulating Vegetative Growth, Conidiation, Sexual Development, and Virulence in Fusarium graminearum
title FgPex3, a Peroxisome Biogenesis Factor, Is Involved in Regulating Vegetative Growth, Conidiation, Sexual Development, and Virulence in Fusarium graminearum
title_full FgPex3, a Peroxisome Biogenesis Factor, Is Involved in Regulating Vegetative Growth, Conidiation, Sexual Development, and Virulence in Fusarium graminearum
title_fullStr FgPex3, a Peroxisome Biogenesis Factor, Is Involved in Regulating Vegetative Growth, Conidiation, Sexual Development, and Virulence in Fusarium graminearum
title_full_unstemmed FgPex3, a Peroxisome Biogenesis Factor, Is Involved in Regulating Vegetative Growth, Conidiation, Sexual Development, and Virulence in Fusarium graminearum
title_short FgPex3, a Peroxisome Biogenesis Factor, Is Involved in Regulating Vegetative Growth, Conidiation, Sexual Development, and Virulence in Fusarium graminearum
title_sort fgpex3, a peroxisome biogenesis factor, is involved in regulating vegetative growth, conidiation, sexual development, and virulence in fusarium graminearum
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764106/
https://www.ncbi.nlm.nih.gov/pubmed/31616386
http://dx.doi.org/10.3389/fmicb.2019.02088
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