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Astragaloside IV attenuates gestational diabetes mellitus via targeting NLRP3 inflammasome in genetic mice

BACKGROUND: As the most ordinary metabolic disorder during pregnancy, gestational diabetes mellitus (GDM) has become a severe risk for the health of both pregnant female and fetus. Astragaloside IV (AS-IV) is the dominant active component in Astragalus membranaceus. It has been proved that AS-IV has...

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Autores principales: Zhang, Ruixue, Zhang, Xuelei, Xing, Baoheng, Zhao, Jianyong, Zhang, Peipei, Shi, Dandan, Yang, Fengzhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764134/
https://www.ncbi.nlm.nih.gov/pubmed/31558153
http://dx.doi.org/10.1186/s12958-019-0522-7
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author Zhang, Ruixue
Zhang, Xuelei
Xing, Baoheng
Zhao, Jianyong
Zhang, Peipei
Shi, Dandan
Yang, Fengzhen
author_facet Zhang, Ruixue
Zhang, Xuelei
Xing, Baoheng
Zhao, Jianyong
Zhang, Peipei
Shi, Dandan
Yang, Fengzhen
author_sort Zhang, Ruixue
collection PubMed
description BACKGROUND: As the most ordinary metabolic disorder during pregnancy, gestational diabetes mellitus (GDM) has become a severe risk for the health of both pregnant female and fetus. Astragaloside IV (AS-IV) is the dominant active component in Astragalus membranaceus. It has been proved that AS-IV has anti-inflammation and immune-regulation function. We aimed to demonstrate the function of AS-IV in the therapy of GDM and the molecular mechanism in this process. METHODS: C57BL/KsJ-Lepdb/+ female mice were used as GDM model. The mRNA levels of relative genes in this research were detected by qRT-PCR. The protein levels of relative genes were analyzed by western blot. Serum concentration of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) were analyzed by ELISA. RESULTS: Glucose and insulin levels in GDM mice model were decreased by AS-IV treatment. AS-IV down-regulated the expression of inflammatory gene IL-6 and TNF-α in GDM mice model. AS-IV treatment inhibited the expression of NLR family pyrin domain containing-3 (NLRP3) inflammasome relative proteins in the pancreas of GDM mice. CONCLUSION: This study demonstrated that AS-IV treatment has an effective therapeutic function of GDM in mice model through the inhibition of NLRP3 inflammasome in the pancreas.
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spelling pubmed-67641342019-09-30 Astragaloside IV attenuates gestational diabetes mellitus via targeting NLRP3 inflammasome in genetic mice Zhang, Ruixue Zhang, Xuelei Xing, Baoheng Zhao, Jianyong Zhang, Peipei Shi, Dandan Yang, Fengzhen Reprod Biol Endocrinol Research BACKGROUND: As the most ordinary metabolic disorder during pregnancy, gestational diabetes mellitus (GDM) has become a severe risk for the health of both pregnant female and fetus. Astragaloside IV (AS-IV) is the dominant active component in Astragalus membranaceus. It has been proved that AS-IV has anti-inflammation and immune-regulation function. We aimed to demonstrate the function of AS-IV in the therapy of GDM and the molecular mechanism in this process. METHODS: C57BL/KsJ-Lepdb/+ female mice were used as GDM model. The mRNA levels of relative genes in this research were detected by qRT-PCR. The protein levels of relative genes were analyzed by western blot. Serum concentration of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) were analyzed by ELISA. RESULTS: Glucose and insulin levels in GDM mice model were decreased by AS-IV treatment. AS-IV down-regulated the expression of inflammatory gene IL-6 and TNF-α in GDM mice model. AS-IV treatment inhibited the expression of NLR family pyrin domain containing-3 (NLRP3) inflammasome relative proteins in the pancreas of GDM mice. CONCLUSION: This study demonstrated that AS-IV treatment has an effective therapeutic function of GDM in mice model through the inhibition of NLRP3 inflammasome in the pancreas. BioMed Central 2019-09-26 /pmc/articles/PMC6764134/ /pubmed/31558153 http://dx.doi.org/10.1186/s12958-019-0522-7 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Zhang, Ruixue
Zhang, Xuelei
Xing, Baoheng
Zhao, Jianyong
Zhang, Peipei
Shi, Dandan
Yang, Fengzhen
Astragaloside IV attenuates gestational diabetes mellitus via targeting NLRP3 inflammasome in genetic mice
title Astragaloside IV attenuates gestational diabetes mellitus via targeting NLRP3 inflammasome in genetic mice
title_full Astragaloside IV attenuates gestational diabetes mellitus via targeting NLRP3 inflammasome in genetic mice
title_fullStr Astragaloside IV attenuates gestational diabetes mellitus via targeting NLRP3 inflammasome in genetic mice
title_full_unstemmed Astragaloside IV attenuates gestational diabetes mellitus via targeting NLRP3 inflammasome in genetic mice
title_short Astragaloside IV attenuates gestational diabetes mellitus via targeting NLRP3 inflammasome in genetic mice
title_sort astragaloside iv attenuates gestational diabetes mellitus via targeting nlrp3 inflammasome in genetic mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764134/
https://www.ncbi.nlm.nih.gov/pubmed/31558153
http://dx.doi.org/10.1186/s12958-019-0522-7
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