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Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America
PREMISE: A novel set of nuclear microsatellite markers was developed and characterized for Campomanesia adamantium (Myrtaceae) and tested for cross‐amplification in the related species C. sessiliflora. METHODS AND RESULTS: Forty‐one primer pairs were designed for simple sequence repeat loci, of whic...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764490/ https://www.ncbi.nlm.nih.gov/pubmed/31572628 http://dx.doi.org/10.1002/aps3.11287 |
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author | Crispim, Bruno do Amaral Déo, Thamiris Gatti Fernandes, Juliana dos Santos de Vasconcelos, Adrielle Ayumi Vieira, Maria do Carmo Carnevali, Thiago de Oliveira Bajay, Miklos Maximiliano Zucchi, Maria Imaculada Barufatti, Alexeia |
author_facet | Crispim, Bruno do Amaral Déo, Thamiris Gatti Fernandes, Juliana dos Santos de Vasconcelos, Adrielle Ayumi Vieira, Maria do Carmo Carnevali, Thiago de Oliveira Bajay, Miklos Maximiliano Zucchi, Maria Imaculada Barufatti, Alexeia |
author_sort | Crispim, Bruno do Amaral |
collection | PubMed |
description | PREMISE: A novel set of nuclear microsatellite markers was developed and characterized for Campomanesia adamantium (Myrtaceae) and tested for cross‐amplification in the related species C. sessiliflora. METHODS AND RESULTS: Forty‐one primer pairs were designed for simple sequence repeat loci, of which 36 successfully amplified and were polymorphic. The number of alleles ranged from two to 14, with an average of 8.14 alleles per locus. Additionally, cross‐amplification was tested in C. sessiliflora; more than 55.5% of the microsatellite loci amplified, confirming the use of these microsatellite markers in a related species. CONCLUSIONS: We developed a set of microsatellite markers that will be useful for future studies of genetic diversity and population structure of C. adamantium and a closely related species, which will aid in future conservation efforts. |
format | Online Article Text |
id | pubmed-6764490 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67644902019-09-30 Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America Crispim, Bruno do Amaral Déo, Thamiris Gatti Fernandes, Juliana dos Santos de Vasconcelos, Adrielle Ayumi Vieira, Maria do Carmo Carnevali, Thiago de Oliveira Bajay, Miklos Maximiliano Zucchi, Maria Imaculada Barufatti, Alexeia Appl Plant Sci Primer Notes PREMISE: A novel set of nuclear microsatellite markers was developed and characterized for Campomanesia adamantium (Myrtaceae) and tested for cross‐amplification in the related species C. sessiliflora. METHODS AND RESULTS: Forty‐one primer pairs were designed for simple sequence repeat loci, of which 36 successfully amplified and were polymorphic. The number of alleles ranged from two to 14, with an average of 8.14 alleles per locus. Additionally, cross‐amplification was tested in C. sessiliflora; more than 55.5% of the microsatellite loci amplified, confirming the use of these microsatellite markers in a related species. CONCLUSIONS: We developed a set of microsatellite markers that will be useful for future studies of genetic diversity and population structure of C. adamantium and a closely related species, which will aid in future conservation efforts. John Wiley and Sons Inc. 2019-09-22 /pmc/articles/PMC6764490/ /pubmed/31572628 http://dx.doi.org/10.1002/aps3.11287 Text en © 2019 Crispim et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Primer Notes Crispim, Bruno do Amaral Déo, Thamiris Gatti Fernandes, Juliana dos Santos de Vasconcelos, Adrielle Ayumi Vieira, Maria do Carmo Carnevali, Thiago de Oliveira Bajay, Miklos Maximiliano Zucchi, Maria Imaculada Barufatti, Alexeia Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America |
title | Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America |
title_full | Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America |
title_fullStr | Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America |
title_full_unstemmed | Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America |
title_short | Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America |
title_sort | development and characterization of microsatellite markers in campomanesia adamantium, a native plant of the cerrado ecoregions of south america |
topic | Primer Notes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764490/ https://www.ncbi.nlm.nih.gov/pubmed/31572628 http://dx.doi.org/10.1002/aps3.11287 |
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