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Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America

PREMISE: A novel set of nuclear microsatellite markers was developed and characterized for Campomanesia adamantium (Myrtaceae) and tested for cross‐amplification in the related species C. sessiliflora. METHODS AND RESULTS: Forty‐one primer pairs were designed for simple sequence repeat loci, of whic...

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Autores principales: Crispim, Bruno do Amaral, Déo, Thamiris Gatti, Fernandes, Juliana dos Santos, de Vasconcelos, Adrielle Ayumi, Vieira, Maria do Carmo, Carnevali, Thiago de Oliveira, Bajay, Miklos Maximiliano, Zucchi, Maria Imaculada, Barufatti, Alexeia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764490/
https://www.ncbi.nlm.nih.gov/pubmed/31572628
http://dx.doi.org/10.1002/aps3.11287
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author Crispim, Bruno do Amaral
Déo, Thamiris Gatti
Fernandes, Juliana dos Santos
de Vasconcelos, Adrielle Ayumi
Vieira, Maria do Carmo
Carnevali, Thiago de Oliveira
Bajay, Miklos Maximiliano
Zucchi, Maria Imaculada
Barufatti, Alexeia
author_facet Crispim, Bruno do Amaral
Déo, Thamiris Gatti
Fernandes, Juliana dos Santos
de Vasconcelos, Adrielle Ayumi
Vieira, Maria do Carmo
Carnevali, Thiago de Oliveira
Bajay, Miklos Maximiliano
Zucchi, Maria Imaculada
Barufatti, Alexeia
author_sort Crispim, Bruno do Amaral
collection PubMed
description PREMISE: A novel set of nuclear microsatellite markers was developed and characterized for Campomanesia adamantium (Myrtaceae) and tested for cross‐amplification in the related species C. sessiliflora. METHODS AND RESULTS: Forty‐one primer pairs were designed for simple sequence repeat loci, of which 36 successfully amplified and were polymorphic. The number of alleles ranged from two to 14, with an average of 8.14 alleles per locus. Additionally, cross‐amplification was tested in C. sessiliflora; more than 55.5% of the microsatellite loci amplified, confirming the use of these microsatellite markers in a related species. CONCLUSIONS: We developed a set of microsatellite markers that will be useful for future studies of genetic diversity and population structure of C. adamantium and a closely related species, which will aid in future conservation efforts.
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spelling pubmed-67644902019-09-30 Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America Crispim, Bruno do Amaral Déo, Thamiris Gatti Fernandes, Juliana dos Santos de Vasconcelos, Adrielle Ayumi Vieira, Maria do Carmo Carnevali, Thiago de Oliveira Bajay, Miklos Maximiliano Zucchi, Maria Imaculada Barufatti, Alexeia Appl Plant Sci Primer Notes PREMISE: A novel set of nuclear microsatellite markers was developed and characterized for Campomanesia adamantium (Myrtaceae) and tested for cross‐amplification in the related species C. sessiliflora. METHODS AND RESULTS: Forty‐one primer pairs were designed for simple sequence repeat loci, of which 36 successfully amplified and were polymorphic. The number of alleles ranged from two to 14, with an average of 8.14 alleles per locus. Additionally, cross‐amplification was tested in C. sessiliflora; more than 55.5% of the microsatellite loci amplified, confirming the use of these microsatellite markers in a related species. CONCLUSIONS: We developed a set of microsatellite markers that will be useful for future studies of genetic diversity and population structure of C. adamantium and a closely related species, which will aid in future conservation efforts. John Wiley and Sons Inc. 2019-09-22 /pmc/articles/PMC6764490/ /pubmed/31572628 http://dx.doi.org/10.1002/aps3.11287 Text en © 2019 Crispim et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Primer Notes
Crispim, Bruno do Amaral
Déo, Thamiris Gatti
Fernandes, Juliana dos Santos
de Vasconcelos, Adrielle Ayumi
Vieira, Maria do Carmo
Carnevali, Thiago de Oliveira
Bajay, Miklos Maximiliano
Zucchi, Maria Imaculada
Barufatti, Alexeia
Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America
title Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America
title_full Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America
title_fullStr Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America
title_full_unstemmed Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America
title_short Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America
title_sort development and characterization of microsatellite markers in campomanesia adamantium, a native plant of the cerrado ecoregions of south america
topic Primer Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764490/
https://www.ncbi.nlm.nih.gov/pubmed/31572628
http://dx.doi.org/10.1002/aps3.11287
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