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Columbamine suppresses the proliferation and malignization of colon cancer cells via abolishing Wnt/β-catenin signaling pathway

BACKGROUND: Colon cancer is one of the most common malignancies worldwide. Because of the side effects and defects in tolerance of chemotherapy, it is necessary to discover new drugs for colon cancer treatment. Columbamine has been identified as an effective anti-osteosarcoma compound with only mino...

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Autores principales: Lei, Changjiang, Yao, Yao, Shen, Bin, Liu, Junru, Pan, Qingyun, Liu, Ning, Li, Lei, Huang, Jianbin, Long, Zhixiong, Shao, Liwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764743/
https://www.ncbi.nlm.nih.gov/pubmed/31572013
http://dx.doi.org/10.2147/CMAR.S209861
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author Lei, Changjiang
Yao, Yao
Shen, Bin
Liu, Junru
Pan, Qingyun
Liu, Ning
Li, Lei
Huang, Jianbin
Long, Zhixiong
Shao, Liwei
author_facet Lei, Changjiang
Yao, Yao
Shen, Bin
Liu, Junru
Pan, Qingyun
Liu, Ning
Li, Lei
Huang, Jianbin
Long, Zhixiong
Shao, Liwei
author_sort Lei, Changjiang
collection PubMed
description BACKGROUND: Colon cancer is one of the most common malignancies worldwide. Because of the side effects and defects in tolerance of chemotherapy, it is necessary to discover new drugs for colon cancer treatment. Columbamine has been identified as an effective anti-osteosarcoma compound with only minor side effects. In this study, we analyzed the anticancer effect of columbamine on colon cancer. METHODS: Human colon cancer cell lines were treatment with columbamine. MTT assay, colony formation assay, apoptosis detection and tumorigenicity assay were performed to detect the protective effect of columbamine on colon cancer development. Western blot assay and luciferase reporter assay were conducted to investigate the potential mechanism of the columbamine treatment. RESULTS: Columbamine significantly inhibited the proliferation, migration, invasion process of colon cancer cells, and dramatically promoted the apoptosis rate of colon cancer cells to further suppress the development of colon cancer to tumor. Both the signaling transducing and key factors expression of Wnt/β-catenin signaling pathway were obviously repressed by columbamine treatment in a dose-dependent manner. CONCLUSION: The present study indicated that columbamine exerts its anti-tumor effect in colon cancer cells through abolishing Wnt/β-catenin signaling pathway. Columbamine may be a new therapy compound for colon cancer.
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spelling pubmed-67647432019-09-30 Columbamine suppresses the proliferation and malignization of colon cancer cells via abolishing Wnt/β-catenin signaling pathway Lei, Changjiang Yao, Yao Shen, Bin Liu, Junru Pan, Qingyun Liu, Ning Li, Lei Huang, Jianbin Long, Zhixiong Shao, Liwei Cancer Manag Res Original Research BACKGROUND: Colon cancer is one of the most common malignancies worldwide. Because of the side effects and defects in tolerance of chemotherapy, it is necessary to discover new drugs for colon cancer treatment. Columbamine has been identified as an effective anti-osteosarcoma compound with only minor side effects. In this study, we analyzed the anticancer effect of columbamine on colon cancer. METHODS: Human colon cancer cell lines were treatment with columbamine. MTT assay, colony formation assay, apoptosis detection and tumorigenicity assay were performed to detect the protective effect of columbamine on colon cancer development. Western blot assay and luciferase reporter assay were conducted to investigate the potential mechanism of the columbamine treatment. RESULTS: Columbamine significantly inhibited the proliferation, migration, invasion process of colon cancer cells, and dramatically promoted the apoptosis rate of colon cancer cells to further suppress the development of colon cancer to tumor. Both the signaling transducing and key factors expression of Wnt/β-catenin signaling pathway were obviously repressed by columbamine treatment in a dose-dependent manner. CONCLUSION: The present study indicated that columbamine exerts its anti-tumor effect in colon cancer cells through abolishing Wnt/β-catenin signaling pathway. Columbamine may be a new therapy compound for colon cancer. Dove 2019-09-23 /pmc/articles/PMC6764743/ /pubmed/31572013 http://dx.doi.org/10.2147/CMAR.S209861 Text en © 2019 Lei et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Lei, Changjiang
Yao, Yao
Shen, Bin
Liu, Junru
Pan, Qingyun
Liu, Ning
Li, Lei
Huang, Jianbin
Long, Zhixiong
Shao, Liwei
Columbamine suppresses the proliferation and malignization of colon cancer cells via abolishing Wnt/β-catenin signaling pathway
title Columbamine suppresses the proliferation and malignization of colon cancer cells via abolishing Wnt/β-catenin signaling pathway
title_full Columbamine suppresses the proliferation and malignization of colon cancer cells via abolishing Wnt/β-catenin signaling pathway
title_fullStr Columbamine suppresses the proliferation and malignization of colon cancer cells via abolishing Wnt/β-catenin signaling pathway
title_full_unstemmed Columbamine suppresses the proliferation and malignization of colon cancer cells via abolishing Wnt/β-catenin signaling pathway
title_short Columbamine suppresses the proliferation and malignization of colon cancer cells via abolishing Wnt/β-catenin signaling pathway
title_sort columbamine suppresses the proliferation and malignization of colon cancer cells via abolishing wnt/β-catenin signaling pathway
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764743/
https://www.ncbi.nlm.nih.gov/pubmed/31572013
http://dx.doi.org/10.2147/CMAR.S209861
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