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UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight

Alterations in light quality significantly affect plant growth and development. In canopy shade, phytochrome photoreceptors perceive reduced ratios of red to far-red light (R:FR) and initiate stem elongation to enable plants to overtop competitors. This shade avoidance response is achieved via the s...

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Autores principales: Sharma, Ashutosh, Sharma, Bhavana, Hayes, Scott, Kerner, Konstantin, Hoecker, Ute, Jenkins, Gareth I., Franklin, Keara A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764944/
https://www.ncbi.nlm.nih.gov/pubmed/31562307
http://dx.doi.org/10.1038/s41467-019-12369-1
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author Sharma, Ashutosh
Sharma, Bhavana
Hayes, Scott
Kerner, Konstantin
Hoecker, Ute
Jenkins, Gareth I.
Franklin, Keara A.
author_facet Sharma, Ashutosh
Sharma, Bhavana
Hayes, Scott
Kerner, Konstantin
Hoecker, Ute
Jenkins, Gareth I.
Franklin, Keara A.
author_sort Sharma, Ashutosh
collection PubMed
description Alterations in light quality significantly affect plant growth and development. In canopy shade, phytochrome photoreceptors perceive reduced ratios of red to far-red light (R:FR) and initiate stem elongation to enable plants to overtop competitors. This shade avoidance response is achieved via the stabilisation and activation of PHYTOCHROME INTERACTING FACTORs (PIFs) which elevate auxin biosynthesis. UV-B inhibits shade avoidance by reducing the abundance and activity of PIFs, yet the molecular mechanisms controlling PIF abundance in UV-B are unknown. Here we show that the UV-B photoreceptor UVR8 promotes rapid PIF5 degradation via the ubiquitin-proteasome system in a response requiring the N terminus of PIF5. In planta interactions between UVR8 and PIF5 are not observed. We further demonstrate that PIF5 interacts with the E3 ligase COP1, promoting PIF5 stabilisation in light-grown plants. Binding of UVR8 to COP1 in UV-B disrupts this stabilisation, providing a mechanism to rapidly lower PIF5 abundance in sunlight.
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spelling pubmed-67649442019-09-30 UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight Sharma, Ashutosh Sharma, Bhavana Hayes, Scott Kerner, Konstantin Hoecker, Ute Jenkins, Gareth I. Franklin, Keara A. Nat Commun Article Alterations in light quality significantly affect plant growth and development. In canopy shade, phytochrome photoreceptors perceive reduced ratios of red to far-red light (R:FR) and initiate stem elongation to enable plants to overtop competitors. This shade avoidance response is achieved via the stabilisation and activation of PHYTOCHROME INTERACTING FACTORs (PIFs) which elevate auxin biosynthesis. UV-B inhibits shade avoidance by reducing the abundance and activity of PIFs, yet the molecular mechanisms controlling PIF abundance in UV-B are unknown. Here we show that the UV-B photoreceptor UVR8 promotes rapid PIF5 degradation via the ubiquitin-proteasome system in a response requiring the N terminus of PIF5. In planta interactions between UVR8 and PIF5 are not observed. We further demonstrate that PIF5 interacts with the E3 ligase COP1, promoting PIF5 stabilisation in light-grown plants. Binding of UVR8 to COP1 in UV-B disrupts this stabilisation, providing a mechanism to rapidly lower PIF5 abundance in sunlight. Nature Publishing Group UK 2019-09-27 /pmc/articles/PMC6764944/ /pubmed/31562307 http://dx.doi.org/10.1038/s41467-019-12369-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sharma, Ashutosh
Sharma, Bhavana
Hayes, Scott
Kerner, Konstantin
Hoecker, Ute
Jenkins, Gareth I.
Franklin, Keara A.
UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight
title UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight
title_full UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight
title_fullStr UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight
title_full_unstemmed UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight
title_short UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight
title_sort uvr8 disrupts stabilisation of pif5 by cop1 to inhibit plant stem elongation in sunlight
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764944/
https://www.ncbi.nlm.nih.gov/pubmed/31562307
http://dx.doi.org/10.1038/s41467-019-12369-1
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