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Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells

Zika virus (ZIKV) invades and persists in the central nervous system (CNS), causing severe neurological diseases. However the virus journey, from the bloodstream to tissues through a mature endothelium, remains unclear. Here, we show that ZIKV-infected monocytes represent suitable carriers for viral...

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Autores principales: Ayala-Nunez, Nilda Vanesa, Follain, Gautier, Delalande, François, Hirschler, Aurélie, Partiot, Emma, Hale, Gillian L., Bollweg, Brigid C., Roels, Judith, Chazal, Maxime, Bakoa, Florian, Carocci, Margot, Bourdoulous, Sandrine, Faklaris, Orestis, Zaki, Sherif R., Eckly, Anita, Uring-Lambert, Béatrice, Doussau, Frédéric, Cianferani, Sarah, Carapito, Christine, Jacobs, Frank M. J., Jouvenet, Nolwenn, Goetz, Jacky G., Gaudin, Raphael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764950/
https://www.ncbi.nlm.nih.gov/pubmed/31562326
http://dx.doi.org/10.1038/s41467-019-12408-x
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author Ayala-Nunez, Nilda Vanesa
Follain, Gautier
Delalande, François
Hirschler, Aurélie
Partiot, Emma
Hale, Gillian L.
Bollweg, Brigid C.
Roels, Judith
Chazal, Maxime
Bakoa, Florian
Carocci, Margot
Bourdoulous, Sandrine
Faklaris, Orestis
Zaki, Sherif R.
Eckly, Anita
Uring-Lambert, Béatrice
Doussau, Frédéric
Cianferani, Sarah
Carapito, Christine
Jacobs, Frank M. J.
Jouvenet, Nolwenn
Goetz, Jacky G.
Gaudin, Raphael
author_facet Ayala-Nunez, Nilda Vanesa
Follain, Gautier
Delalande, François
Hirschler, Aurélie
Partiot, Emma
Hale, Gillian L.
Bollweg, Brigid C.
Roels, Judith
Chazal, Maxime
Bakoa, Florian
Carocci, Margot
Bourdoulous, Sandrine
Faklaris, Orestis
Zaki, Sherif R.
Eckly, Anita
Uring-Lambert, Béatrice
Doussau, Frédéric
Cianferani, Sarah
Carapito, Christine
Jacobs, Frank M. J.
Jouvenet, Nolwenn
Goetz, Jacky G.
Gaudin, Raphael
author_sort Ayala-Nunez, Nilda Vanesa
collection PubMed
description Zika virus (ZIKV) invades and persists in the central nervous system (CNS), causing severe neurological diseases. However the virus journey, from the bloodstream to tissues through a mature endothelium, remains unclear. Here, we show that ZIKV-infected monocytes represent suitable carriers for viral dissemination to the CNS using human primary monocytes, cerebral organoids derived from embryonic stem cells, organotypic mouse cerebellar slices, a xenotypic human-zebrafish model, and human fetus brain samples. We find that ZIKV-exposed monocytes exhibit higher expression of adhesion molecules, and higher abilities to attach onto the vessel wall and transmigrate across endothelia. This phenotype is associated to enhanced monocyte-mediated ZIKV dissemination to neural cells. Together, our data show that ZIKV manipulates the monocyte adhesive properties and enhances monocyte transmigration and viral dissemination to neural cells. Monocyte transmigration may represent an important mechanism required for viral tissue invasion and persistence that could be specifically targeted for therapeutic intervention.
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spelling pubmed-67649502019-09-30 Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells Ayala-Nunez, Nilda Vanesa Follain, Gautier Delalande, François Hirschler, Aurélie Partiot, Emma Hale, Gillian L. Bollweg, Brigid C. Roels, Judith Chazal, Maxime Bakoa, Florian Carocci, Margot Bourdoulous, Sandrine Faklaris, Orestis Zaki, Sherif R. Eckly, Anita Uring-Lambert, Béatrice Doussau, Frédéric Cianferani, Sarah Carapito, Christine Jacobs, Frank M. J. Jouvenet, Nolwenn Goetz, Jacky G. Gaudin, Raphael Nat Commun Article Zika virus (ZIKV) invades and persists in the central nervous system (CNS), causing severe neurological diseases. However the virus journey, from the bloodstream to tissues through a mature endothelium, remains unclear. Here, we show that ZIKV-infected monocytes represent suitable carriers for viral dissemination to the CNS using human primary monocytes, cerebral organoids derived from embryonic stem cells, organotypic mouse cerebellar slices, a xenotypic human-zebrafish model, and human fetus brain samples. We find that ZIKV-exposed monocytes exhibit higher expression of adhesion molecules, and higher abilities to attach onto the vessel wall and transmigrate across endothelia. This phenotype is associated to enhanced monocyte-mediated ZIKV dissemination to neural cells. Together, our data show that ZIKV manipulates the monocyte adhesive properties and enhances monocyte transmigration and viral dissemination to neural cells. Monocyte transmigration may represent an important mechanism required for viral tissue invasion and persistence that could be specifically targeted for therapeutic intervention. Nature Publishing Group UK 2019-09-27 /pmc/articles/PMC6764950/ /pubmed/31562326 http://dx.doi.org/10.1038/s41467-019-12408-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ayala-Nunez, Nilda Vanesa
Follain, Gautier
Delalande, François
Hirschler, Aurélie
Partiot, Emma
Hale, Gillian L.
Bollweg, Brigid C.
Roels, Judith
Chazal, Maxime
Bakoa, Florian
Carocci, Margot
Bourdoulous, Sandrine
Faklaris, Orestis
Zaki, Sherif R.
Eckly, Anita
Uring-Lambert, Béatrice
Doussau, Frédéric
Cianferani, Sarah
Carapito, Christine
Jacobs, Frank M. J.
Jouvenet, Nolwenn
Goetz, Jacky G.
Gaudin, Raphael
Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells
title Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells
title_full Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells
title_fullStr Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells
title_full_unstemmed Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells
title_short Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells
title_sort zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764950/
https://www.ncbi.nlm.nih.gov/pubmed/31562326
http://dx.doi.org/10.1038/s41467-019-12408-x
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