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High-definition spatial transcriptomics for in situ tissue profiling
Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed High-Definition Spatial Transcriptomics (HDST), which captures RNA from histological tissue sections on a dense spatially barcoded bead array. Eac...
| Autores principales: | , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6765407/ https://www.ncbi.nlm.nih.gov/pubmed/31501547 http://dx.doi.org/10.1038/s41592-019-0548-y |
| _version_ | 1783454551108485120 |
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| author | Vickovic, Sanja Eraslan, Gökcen Salmén, Fredrik Klughammer, Johanna Stenbeck, Linnea Schapiro, Denis Äijö, Tarmo Bonneau, Richard Bergenstråhle, Ludvig Navarro, José Fernandéz Gould, Joshua Griffin, Gabriel K. Borg, Åke Ronaghi, Mostafa Frisén, Jonas Lundeberg, Joakim Regev, Aviv Ståhl, Patrik L |
| author_facet | Vickovic, Sanja Eraslan, Gökcen Salmén, Fredrik Klughammer, Johanna Stenbeck, Linnea Schapiro, Denis Äijö, Tarmo Bonneau, Richard Bergenstråhle, Ludvig Navarro, José Fernandéz Gould, Joshua Griffin, Gabriel K. Borg, Åke Ronaghi, Mostafa Frisén, Jonas Lundeberg, Joakim Regev, Aviv Ståhl, Patrik L |
| author_sort | Vickovic, Sanja |
| collection | PubMed |
| description | Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed High-Definition Spatial Transcriptomics (HDST), which captures RNA from histological tissue sections on a dense spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues. |
| format | Online Article Text |
| id | pubmed-6765407 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2019 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-67654072020-03-09 High-definition spatial transcriptomics for in situ tissue profiling Vickovic, Sanja Eraslan, Gökcen Salmén, Fredrik Klughammer, Johanna Stenbeck, Linnea Schapiro, Denis Äijö, Tarmo Bonneau, Richard Bergenstråhle, Ludvig Navarro, José Fernandéz Gould, Joshua Griffin, Gabriel K. Borg, Åke Ronaghi, Mostafa Frisén, Jonas Lundeberg, Joakim Regev, Aviv Ståhl, Patrik L Nat Methods Article Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed High-Definition Spatial Transcriptomics (HDST), which captures RNA from histological tissue sections on a dense spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues. 2019-09-09 2019-10 /pmc/articles/PMC6765407/ /pubmed/31501547 http://dx.doi.org/10.1038/s41592-019-0548-y Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Vickovic, Sanja Eraslan, Gökcen Salmén, Fredrik Klughammer, Johanna Stenbeck, Linnea Schapiro, Denis Äijö, Tarmo Bonneau, Richard Bergenstråhle, Ludvig Navarro, José Fernandéz Gould, Joshua Griffin, Gabriel K. Borg, Åke Ronaghi, Mostafa Frisén, Jonas Lundeberg, Joakim Regev, Aviv Ståhl, Patrik L High-definition spatial transcriptomics for in situ tissue profiling |
| title | High-definition spatial transcriptomics for in situ tissue profiling |
| title_full | High-definition spatial transcriptomics for in situ tissue profiling |
| title_fullStr | High-definition spatial transcriptomics for in situ tissue profiling |
| title_full_unstemmed | High-definition spatial transcriptomics for in situ tissue profiling |
| title_short | High-definition spatial transcriptomics for in situ tissue profiling |
| title_sort | high-definition spatial transcriptomics for in situ tissue profiling |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6765407/ https://www.ncbi.nlm.nih.gov/pubmed/31501547 http://dx.doi.org/10.1038/s41592-019-0548-y |
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