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Evaluation of Fresh Water Actinomycete Bioflocculant and Its Biotechnological Applications in Wastewaters Treatment and Removal of Heavy Metals

This study evaluated the potential of a biopolymeric flocculant produced by Terrabacter sp. isolated from Sterkfontein Dam, South Africa. Microbial flocculants aid the aggregation of suspended solutes in solutions, thus, suggesting its alternative application to inorganic and synthetic organic flocc...

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Autores principales: Agunbiade, Mayowa Oladele, Pohl, Carolina, Heerden, Esta Van, Oyekola, Oluwaseun, Ashafa, Anofi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6765771/
https://www.ncbi.nlm.nih.gov/pubmed/31510036
http://dx.doi.org/10.3390/ijerph16183337
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author Agunbiade, Mayowa Oladele
Pohl, Carolina
Heerden, Esta Van
Oyekola, Oluwaseun
Ashafa, Anofi
author_facet Agunbiade, Mayowa Oladele
Pohl, Carolina
Heerden, Esta Van
Oyekola, Oluwaseun
Ashafa, Anofi
author_sort Agunbiade, Mayowa Oladele
collection PubMed
description This study evaluated the potential of a biopolymeric flocculant produced by Terrabacter sp. isolated from Sterkfontein Dam, South Africa. Microbial flocculants aid the aggregation of suspended solutes in solutions, thus, suggesting its alternative application to inorganic and synthetic organic flocculants, which are associated with health-related problems. The 16S rDNA analysis revealed the bacteria to have 98% similarity to Terrabacter sp. MUSC78T and the sequence was deposited in the Genbank as Terrabacter sp. with accession number KF682157.1. A series of experimental parameters such as bioflocculant dosage, cations concentrations, pH, and application of the purified bioflocculant in wastewaters treatment were investigated. In the presence of glucose as a sole carbon source, Ca(2+) as cation at pH 8, the optimal flocculating activity attained was 85%. Optimum bioflocculant dosage of 0.5 mg/mL was able to remove chemical oxygen demand (COD), biological oxygen demand (BOD), suspended solids (SS), nitrate, and turbidity in dairy wastewater. In addition, the tested bioflocculant exhibited higher flocculating efficiency as compared to polyaluminum chloride, polyethylenime, and alum. Inductible coupled plasma optical emission spectroscopy (ICP-OES) analyses confirmed significant removal of 77.7% Fe, 74.8% Al, 61.9% Mn, and 57.6% Zn as representatives of heavy metals from treated dairy wastewater. Fourier transform infrared spectroscopy (FTIR) indicated the presence of carboxyl, hydroxyl, and amino groups in the purified bioflocculant which could be responsible for flocculation. Findings from this study showed the prospect of the studied bioflocculant as an alternative candidate in wastewater treatment and remediating of heavy metals.
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spelling pubmed-67657712019-09-30 Evaluation of Fresh Water Actinomycete Bioflocculant and Its Biotechnological Applications in Wastewaters Treatment and Removal of Heavy Metals Agunbiade, Mayowa Oladele Pohl, Carolina Heerden, Esta Van Oyekola, Oluwaseun Ashafa, Anofi Int J Environ Res Public Health Article This study evaluated the potential of a biopolymeric flocculant produced by Terrabacter sp. isolated from Sterkfontein Dam, South Africa. Microbial flocculants aid the aggregation of suspended solutes in solutions, thus, suggesting its alternative application to inorganic and synthetic organic flocculants, which are associated with health-related problems. The 16S rDNA analysis revealed the bacteria to have 98% similarity to Terrabacter sp. MUSC78T and the sequence was deposited in the Genbank as Terrabacter sp. with accession number KF682157.1. A series of experimental parameters such as bioflocculant dosage, cations concentrations, pH, and application of the purified bioflocculant in wastewaters treatment were investigated. In the presence of glucose as a sole carbon source, Ca(2+) as cation at pH 8, the optimal flocculating activity attained was 85%. Optimum bioflocculant dosage of 0.5 mg/mL was able to remove chemical oxygen demand (COD), biological oxygen demand (BOD), suspended solids (SS), nitrate, and turbidity in dairy wastewater. In addition, the tested bioflocculant exhibited higher flocculating efficiency as compared to polyaluminum chloride, polyethylenime, and alum. Inductible coupled plasma optical emission spectroscopy (ICP-OES) analyses confirmed significant removal of 77.7% Fe, 74.8% Al, 61.9% Mn, and 57.6% Zn as representatives of heavy metals from treated dairy wastewater. Fourier transform infrared spectroscopy (FTIR) indicated the presence of carboxyl, hydroxyl, and amino groups in the purified bioflocculant which could be responsible for flocculation. Findings from this study showed the prospect of the studied bioflocculant as an alternative candidate in wastewater treatment and remediating of heavy metals. MDPI 2019-09-10 2019-09 /pmc/articles/PMC6765771/ /pubmed/31510036 http://dx.doi.org/10.3390/ijerph16183337 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Agunbiade, Mayowa Oladele
Pohl, Carolina
Heerden, Esta Van
Oyekola, Oluwaseun
Ashafa, Anofi
Evaluation of Fresh Water Actinomycete Bioflocculant and Its Biotechnological Applications in Wastewaters Treatment and Removal of Heavy Metals
title Evaluation of Fresh Water Actinomycete Bioflocculant and Its Biotechnological Applications in Wastewaters Treatment and Removal of Heavy Metals
title_full Evaluation of Fresh Water Actinomycete Bioflocculant and Its Biotechnological Applications in Wastewaters Treatment and Removal of Heavy Metals
title_fullStr Evaluation of Fresh Water Actinomycete Bioflocculant and Its Biotechnological Applications in Wastewaters Treatment and Removal of Heavy Metals
title_full_unstemmed Evaluation of Fresh Water Actinomycete Bioflocculant and Its Biotechnological Applications in Wastewaters Treatment and Removal of Heavy Metals
title_short Evaluation of Fresh Water Actinomycete Bioflocculant and Its Biotechnological Applications in Wastewaters Treatment and Removal of Heavy Metals
title_sort evaluation of fresh water actinomycete bioflocculant and its biotechnological applications in wastewaters treatment and removal of heavy metals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6765771/
https://www.ncbi.nlm.nih.gov/pubmed/31510036
http://dx.doi.org/10.3390/ijerph16183337
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