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Pathological Features of Diabetic Retinopathy in Spontaneously Diabetic Torii Fatty Rats
OBJECTIVE: The Spontaneously Diabetic Torii (SDT) fatty rat, established by introducing the fa allele (obesity gene) of the Zucker fatty rat into the SDT rat genome, is a new model of obese type 2 diabetes. We studied the pathologic features of diabetic retinopathy (DR) in this animal. METHODS: The...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6766157/ https://www.ncbi.nlm.nih.gov/pubmed/31637263 http://dx.doi.org/10.1155/2019/8724818 |
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author | Tanaka, Yoshiaki Takagi, Rina Ohta, Takeshi Sasase, Tomohiko Kobayashi, Mina Toyoda, Fumihiko Shimmura, Machiko Kinoshita, Nozomi Takano, Hiroko Kakehashi, Akihiro |
author_facet | Tanaka, Yoshiaki Takagi, Rina Ohta, Takeshi Sasase, Tomohiko Kobayashi, Mina Toyoda, Fumihiko Shimmura, Machiko Kinoshita, Nozomi Takano, Hiroko Kakehashi, Akihiro |
author_sort | Tanaka, Yoshiaki |
collection | PubMed |
description | OBJECTIVE: The Spontaneously Diabetic Torii (SDT) fatty rat, established by introducing the fa allele (obesity gene) of the Zucker fatty rat into the SDT rat genome, is a new model of obese type 2 diabetes. We studied the pathologic features of diabetic retinopathy (DR) in this animal. METHODS: The eyes of SDT fatty, SDT (controls), and Sprague Dawley (SD) rats (normal controls) were enucleated at 8, 16, 24, 32, and 40 weeks of age (n = 5‐6 for each rat type at each age). The retinal thicknesses, numbers of retinal folds, and choroidal thicknesses were evaluated. Immunostaining for glial fibrillary acidic protein (GFAP) and vascular endothelial growth factor (VEGF) was performed. Quantitative analyses of the immunopositive regions were performed using a cell-counting algorithm. RESULTS: The retinas tended to be thicker in the SDT fatty rats and SDT rats than in the SD rats; the choroids tended to be thicker in the SDT fatty rats than in the SD rats. The retinal folds in the SDT fatty rats developed earlier and were more severe than in the SDT rats. Quantitative analyses showed that the GFAP- and VEGF-positive regions in the retinas of the SDT fatty rats were significantly larger than those of the SDT rats. CONCLUSIONS: SDT fatty rats developed more severe DR earlier than the SDT rats. The SDT fatty rats might be useful as a type 2 diabetes animal model to study DR. |
format | Online Article Text |
id | pubmed-6766157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-67661572019-10-21 Pathological Features of Diabetic Retinopathy in Spontaneously Diabetic Torii Fatty Rats Tanaka, Yoshiaki Takagi, Rina Ohta, Takeshi Sasase, Tomohiko Kobayashi, Mina Toyoda, Fumihiko Shimmura, Machiko Kinoshita, Nozomi Takano, Hiroko Kakehashi, Akihiro J Diabetes Res Research Article OBJECTIVE: The Spontaneously Diabetic Torii (SDT) fatty rat, established by introducing the fa allele (obesity gene) of the Zucker fatty rat into the SDT rat genome, is a new model of obese type 2 diabetes. We studied the pathologic features of diabetic retinopathy (DR) in this animal. METHODS: The eyes of SDT fatty, SDT (controls), and Sprague Dawley (SD) rats (normal controls) were enucleated at 8, 16, 24, 32, and 40 weeks of age (n = 5‐6 for each rat type at each age). The retinal thicknesses, numbers of retinal folds, and choroidal thicknesses were evaluated. Immunostaining for glial fibrillary acidic protein (GFAP) and vascular endothelial growth factor (VEGF) was performed. Quantitative analyses of the immunopositive regions were performed using a cell-counting algorithm. RESULTS: The retinas tended to be thicker in the SDT fatty rats and SDT rats than in the SD rats; the choroids tended to be thicker in the SDT fatty rats than in the SD rats. The retinal folds in the SDT fatty rats developed earlier and were more severe than in the SDT rats. Quantitative analyses showed that the GFAP- and VEGF-positive regions in the retinas of the SDT fatty rats were significantly larger than those of the SDT rats. CONCLUSIONS: SDT fatty rats developed more severe DR earlier than the SDT rats. The SDT fatty rats might be useful as a type 2 diabetes animal model to study DR. Hindawi 2019-09-15 /pmc/articles/PMC6766157/ /pubmed/31637263 http://dx.doi.org/10.1155/2019/8724818 Text en Copyright © 2019 Yoshiaki Tanaka et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Tanaka, Yoshiaki Takagi, Rina Ohta, Takeshi Sasase, Tomohiko Kobayashi, Mina Toyoda, Fumihiko Shimmura, Machiko Kinoshita, Nozomi Takano, Hiroko Kakehashi, Akihiro Pathological Features of Diabetic Retinopathy in Spontaneously Diabetic Torii Fatty Rats |
title | Pathological Features of Diabetic Retinopathy in Spontaneously Diabetic Torii Fatty Rats |
title_full | Pathological Features of Diabetic Retinopathy in Spontaneously Diabetic Torii Fatty Rats |
title_fullStr | Pathological Features of Diabetic Retinopathy in Spontaneously Diabetic Torii Fatty Rats |
title_full_unstemmed | Pathological Features of Diabetic Retinopathy in Spontaneously Diabetic Torii Fatty Rats |
title_short | Pathological Features of Diabetic Retinopathy in Spontaneously Diabetic Torii Fatty Rats |
title_sort | pathological features of diabetic retinopathy in spontaneously diabetic torii fatty rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6766157/ https://www.ncbi.nlm.nih.gov/pubmed/31637263 http://dx.doi.org/10.1155/2019/8724818 |
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