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Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response

Bone infections are a frequent cause for large bony defects with a reduced healing capacity. In previous findings, we could already show diminished healing capacity after bone infections, despite the absence of the causing agent, Staphylococcus aureus. Moreover, these bony defects showed reduced ost...

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Autores principales: Wagner, Johannes Maximilian, Reinkemeier, Felix, Wallner, Christoph, Dadras, Mehran, Huber, Julika, Schmidt, Sonja Verena, Drysch, Marius, Dittfeld, Stephanie, Jaurich, Henriette, Becerikli, Mustafa, Becker, Kathrin, Rauch, Nicole, Duhan, Vikas, Lehnhardt, Marcus, Behr, Björn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6766598/
https://www.ncbi.nlm.nih.gov/pubmed/31179644
http://dx.doi.org/10.1002/sctm.18-0266
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author Wagner, Johannes Maximilian
Reinkemeier, Felix
Wallner, Christoph
Dadras, Mehran
Huber, Julika
Schmidt, Sonja Verena
Drysch, Marius
Dittfeld, Stephanie
Jaurich, Henriette
Becerikli, Mustafa
Becker, Kathrin
Rauch, Nicole
Duhan, Vikas
Lehnhardt, Marcus
Behr, Björn
author_facet Wagner, Johannes Maximilian
Reinkemeier, Felix
Wallner, Christoph
Dadras, Mehran
Huber, Julika
Schmidt, Sonja Verena
Drysch, Marius
Dittfeld, Stephanie
Jaurich, Henriette
Becerikli, Mustafa
Becker, Kathrin
Rauch, Nicole
Duhan, Vikas
Lehnhardt, Marcus
Behr, Björn
author_sort Wagner, Johannes Maximilian
collection PubMed
description Bone infections are a frequent cause for large bony defects with a reduced healing capacity. In previous findings, we could already show diminished healing capacity after bone infections, despite the absence of the causing agent, Staphylococcus aureus. Moreover, these bony defects showed reduced osteoblastogenesis and increased osteoclastogenesis, meaning elevated bone resorption ongoing with an elevated B‐cell activity. To overcome the negative effects of this postinfectious inflammatory state, we tried to use the regenerative capacity of mesenchymal stem cells derived from adipose tissue (adipose‐derived stem cells [ASCs]) to improve bone regeneration and moreover were curious about immunomodulation of applicated stem cells in this setting. Therefore, we used our established murine animal model and applicated ASCs locally after sufficient debridement of infected bones. Bone regeneration and resorption as well as immunological markers were investigated via histology, immunohistochemistry, Western blot, and fluorescence‐activated cell scanning (FACS) analysis and μ‐computed tomography (CT) analysis. Interestingly, ASCs were able to restore bone healing via elevation of osteoblastogenesis and downregulation of osteoclasts. Surprisingly, stem cells showed an impact on the innate immune system, downregulating B‐cell population. In summary, these data provide a fascinating new and innovative approach, supporting bone healing after bacterial infections and moreover gain insights into the complex ceremony of stem cell interaction in terms of bone infection and regeneration. stem cells translational medicine 2019;8:1084–1091
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spelling pubmed-67665982019-09-30 Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response Wagner, Johannes Maximilian Reinkemeier, Felix Wallner, Christoph Dadras, Mehran Huber, Julika Schmidt, Sonja Verena Drysch, Marius Dittfeld, Stephanie Jaurich, Henriette Becerikli, Mustafa Becker, Kathrin Rauch, Nicole Duhan, Vikas Lehnhardt, Marcus Behr, Björn Stem Cells Transl Med Tissue Engineering and Regenerative Medicine Bone infections are a frequent cause for large bony defects with a reduced healing capacity. In previous findings, we could already show diminished healing capacity after bone infections, despite the absence of the causing agent, Staphylococcus aureus. Moreover, these bony defects showed reduced osteoblastogenesis and increased osteoclastogenesis, meaning elevated bone resorption ongoing with an elevated B‐cell activity. To overcome the negative effects of this postinfectious inflammatory state, we tried to use the regenerative capacity of mesenchymal stem cells derived from adipose tissue (adipose‐derived stem cells [ASCs]) to improve bone regeneration and moreover were curious about immunomodulation of applicated stem cells in this setting. Therefore, we used our established murine animal model and applicated ASCs locally after sufficient debridement of infected bones. Bone regeneration and resorption as well as immunological markers were investigated via histology, immunohistochemistry, Western blot, and fluorescence‐activated cell scanning (FACS) analysis and μ‐computed tomography (CT) analysis. Interestingly, ASCs were able to restore bone healing via elevation of osteoblastogenesis and downregulation of osteoclasts. Surprisingly, stem cells showed an impact on the innate immune system, downregulating B‐cell population. In summary, these data provide a fascinating new and innovative approach, supporting bone healing after bacterial infections and moreover gain insights into the complex ceremony of stem cell interaction in terms of bone infection and regeneration. stem cells translational medicine 2019;8:1084–1091 John Wiley & Sons, Inc. 2019-06-10 /pmc/articles/PMC6766598/ /pubmed/31179644 http://dx.doi.org/10.1002/sctm.18-0266 Text en © 2019 The Authors. stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Tissue Engineering and Regenerative Medicine
Wagner, Johannes Maximilian
Reinkemeier, Felix
Wallner, Christoph
Dadras, Mehran
Huber, Julika
Schmidt, Sonja Verena
Drysch, Marius
Dittfeld, Stephanie
Jaurich, Henriette
Becerikli, Mustafa
Becker, Kathrin
Rauch, Nicole
Duhan, Vikas
Lehnhardt, Marcus
Behr, Björn
Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response
title Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response
title_full Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response
title_fullStr Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response
title_full_unstemmed Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response
title_short Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response
title_sort adipose‐derived stromal cells are capable of restoring bone regeneration after post‐traumatic osteomyelitis and modulate b‐cell response
topic Tissue Engineering and Regenerative Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6766598/
https://www.ncbi.nlm.nih.gov/pubmed/31179644
http://dx.doi.org/10.1002/sctm.18-0266
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