Cargando…
Constituents and Anti-Hyperuricemia Mechanism of Traditional Chinese Herbal Formulae Erding Granule
Erding granule (EDG) is a traditional Chinese medicine that has recently been identified as having anti-hypouricemic effects. However, the active components and underlying mechanism for this new indication have not been elucidated. Therefore, we compared the effects of different EDG extracts (water,...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6766821/ https://www.ncbi.nlm.nih.gov/pubmed/31489932 http://dx.doi.org/10.3390/molecules24183248 |
_version_ | 1783454775400988672 |
---|---|
author | Zhang, Wugang Du, Wendi Li, Guofeng Zhang, Chen Yang, Wuliang Yang, Shilin Feng, Yulin Chen, Haifang |
author_facet | Zhang, Wugang Du, Wendi Li, Guofeng Zhang, Chen Yang, Wuliang Yang, Shilin Feng, Yulin Chen, Haifang |
author_sort | Zhang, Wugang |
collection | PubMed |
description | Erding granule (EDG) is a traditional Chinese medicine that has recently been identified as having anti-hypouricemic effects. However, the active components and underlying mechanism for this new indication have not been elucidated. Therefore, we compared the effects of different EDG extracts (water, 50% ethanol and 95% ethanol) on serum uric acid concentrations in the hyperuricemia model mouse. We also analyzed the constituents of different extracts by ultra-high performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) to observe the variation between the active and inactive products. Extract activity and target site were evaluated by assessing uric acid- and inflammation-suppressing effects along with evaluating ability to regulate the uric acid transporter. The results showed that the 50% ethanol extract (EDG-50) had an obvious serum uric acid concentration lowering effect compared with water (EDG-S) and the 95% ethanol extract (EDG-95). UHPLC-Q-TOF-MS/MS analysis showed that EDG-50 was compositionally different to EDG-S and EDG-95. EDG-50 showed dose-dependent effects on reducing uric acid, suppressing inflammation and regulating uric acid transporters. Moreover, western blot analysis showed that EDG-50 down-regulated GLUT9 and URAT1 expression, and up-regulated OAT1 expression. Therefore, our findings enable the preliminarily conclusion that EDG-50 lowers serum uric acid concentrations, mainly by down-regulating the expression of GLUT9 and URAT1 proteins and up-regulating the expression of OAT1 proteins. This provides a research basis for clinical use of EDG as an anti-hyperuricemic agent. |
format | Online Article Text |
id | pubmed-6766821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67668212019-10-02 Constituents and Anti-Hyperuricemia Mechanism of Traditional Chinese Herbal Formulae Erding Granule Zhang, Wugang Du, Wendi Li, Guofeng Zhang, Chen Yang, Wuliang Yang, Shilin Feng, Yulin Chen, Haifang Molecules Article Erding granule (EDG) is a traditional Chinese medicine that has recently been identified as having anti-hypouricemic effects. However, the active components and underlying mechanism for this new indication have not been elucidated. Therefore, we compared the effects of different EDG extracts (water, 50% ethanol and 95% ethanol) on serum uric acid concentrations in the hyperuricemia model mouse. We also analyzed the constituents of different extracts by ultra-high performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) to observe the variation between the active and inactive products. Extract activity and target site were evaluated by assessing uric acid- and inflammation-suppressing effects along with evaluating ability to regulate the uric acid transporter. The results showed that the 50% ethanol extract (EDG-50) had an obvious serum uric acid concentration lowering effect compared with water (EDG-S) and the 95% ethanol extract (EDG-95). UHPLC-Q-TOF-MS/MS analysis showed that EDG-50 was compositionally different to EDG-S and EDG-95. EDG-50 showed dose-dependent effects on reducing uric acid, suppressing inflammation and regulating uric acid transporters. Moreover, western blot analysis showed that EDG-50 down-regulated GLUT9 and URAT1 expression, and up-regulated OAT1 expression. Therefore, our findings enable the preliminarily conclusion that EDG-50 lowers serum uric acid concentrations, mainly by down-regulating the expression of GLUT9 and URAT1 proteins and up-regulating the expression of OAT1 proteins. This provides a research basis for clinical use of EDG as an anti-hyperuricemic agent. MDPI 2019-09-06 /pmc/articles/PMC6766821/ /pubmed/31489932 http://dx.doi.org/10.3390/molecules24183248 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Wugang Du, Wendi Li, Guofeng Zhang, Chen Yang, Wuliang Yang, Shilin Feng, Yulin Chen, Haifang Constituents and Anti-Hyperuricemia Mechanism of Traditional Chinese Herbal Formulae Erding Granule |
title | Constituents and Anti-Hyperuricemia Mechanism of Traditional Chinese Herbal Formulae Erding Granule |
title_full | Constituents and Anti-Hyperuricemia Mechanism of Traditional Chinese Herbal Formulae Erding Granule |
title_fullStr | Constituents and Anti-Hyperuricemia Mechanism of Traditional Chinese Herbal Formulae Erding Granule |
title_full_unstemmed | Constituents and Anti-Hyperuricemia Mechanism of Traditional Chinese Herbal Formulae Erding Granule |
title_short | Constituents and Anti-Hyperuricemia Mechanism of Traditional Chinese Herbal Formulae Erding Granule |
title_sort | constituents and anti-hyperuricemia mechanism of traditional chinese herbal formulae erding granule |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6766821/ https://www.ncbi.nlm.nih.gov/pubmed/31489932 http://dx.doi.org/10.3390/molecules24183248 |
work_keys_str_mv | AT zhangwugang constituentsandantihyperuricemiamechanismoftraditionalchineseherbalformulaeerdinggranule AT duwendi constituentsandantihyperuricemiamechanismoftraditionalchineseherbalformulaeerdinggranule AT liguofeng constituentsandantihyperuricemiamechanismoftraditionalchineseherbalformulaeerdinggranule AT zhangchen constituentsandantihyperuricemiamechanismoftraditionalchineseherbalformulaeerdinggranule AT yangwuliang constituentsandantihyperuricemiamechanismoftraditionalchineseherbalformulaeerdinggranule AT yangshilin constituentsandantihyperuricemiamechanismoftraditionalchineseherbalformulaeerdinggranule AT fengyulin constituentsandantihyperuricemiamechanismoftraditionalchineseherbalformulaeerdinggranule AT chenhaifang constituentsandantihyperuricemiamechanismoftraditionalchineseherbalformulaeerdinggranule |