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In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

Thymus sipyleus Boiss. subsp. rosulans (Borbas) Jalas (TS) is a commonly used plant in the treatment of various complaints, including skin wounds in Turkish folk medicine. Despite the widespread traditional use of TS, there is not any scientific report confirming the effectiveness of this plant on t...

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Autores principales: Ustuner, Oya, Anlas, Ceren, Bakirel, Tulay, Ustun-Alkan, Fulya, Diren Sigirci, Belgi, Ak, Seyyal, Akpulat, Huseyin Askin, Donmez, Ceylan, Koca-Caliskan, Ufuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767006/
https://www.ncbi.nlm.nih.gov/pubmed/31540139
http://dx.doi.org/10.3390/molecules24183353
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author Ustuner, Oya
Anlas, Ceren
Bakirel, Tulay
Ustun-Alkan, Fulya
Diren Sigirci, Belgi
Ak, Seyyal
Akpulat, Huseyin Askin
Donmez, Ceylan
Koca-Caliskan, Ufuk
author_facet Ustuner, Oya
Anlas, Ceren
Bakirel, Tulay
Ustun-Alkan, Fulya
Diren Sigirci, Belgi
Ak, Seyyal
Akpulat, Huseyin Askin
Donmez, Ceylan
Koca-Caliskan, Ufuk
author_sort Ustuner, Oya
collection PubMed
description Thymus sipyleus Boiss. subsp. rosulans (Borbas) Jalas (TS) is a commonly used plant in the treatment of various complaints, including skin wounds in Turkish folk medicine. Despite the widespread traditional use of TS, there is not any scientific report confirming the effectiveness of this plant on the healing process. This research aimed to investigate the effects of different extracts obtained from TS on biological events during wound healing, on a cellular basis. In this context, proliferative activities of the extracts, as well as the effects on wound closure and hydroxyproline synthesis, were determined. In addition to wound healing properties, the antioxidant, antibacterial and anti-inflammatory activities of the extracts were evaluated. Decoction (D) and infusion (I) extracts contained the highest amount of phenolic content and showed the most potent activity against DPPH radical. All extracts exhibited complete protection against the damage induced by hydrogen peroxide (H(2)O(2)) by increasing cell viability compared to only H(2)O(2)-treated groups, both in co-treatment and pre-treatment protocols. None of the extracts exhibited cytotoxic activity, and most of the extracts from the TS stimulated fibroblast proliferation and migration. All TS extracts exert anti-inflammatory activity by suppressing the overproduction of tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO). The most pronounced activity on hydroxyproline synthesis was observed in D extract. In summary, it was observed that TS extracts can promote the healing process by enhancing fibroblast migration, proliferation and collagen synthesis as well as suppressing pro-inflammatory cytokines. The obtained data in this work support the traditional use of TS as a valuable plant-based compound for the treatment of wounds.
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spelling pubmed-67670062019-10-02 In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas Ustuner, Oya Anlas, Ceren Bakirel, Tulay Ustun-Alkan, Fulya Diren Sigirci, Belgi Ak, Seyyal Akpulat, Huseyin Askin Donmez, Ceylan Koca-Caliskan, Ufuk Molecules Article Thymus sipyleus Boiss. subsp. rosulans (Borbas) Jalas (TS) is a commonly used plant in the treatment of various complaints, including skin wounds in Turkish folk medicine. Despite the widespread traditional use of TS, there is not any scientific report confirming the effectiveness of this plant on the healing process. This research aimed to investigate the effects of different extracts obtained from TS on biological events during wound healing, on a cellular basis. In this context, proliferative activities of the extracts, as well as the effects on wound closure and hydroxyproline synthesis, were determined. In addition to wound healing properties, the antioxidant, antibacterial and anti-inflammatory activities of the extracts were evaluated. Decoction (D) and infusion (I) extracts contained the highest amount of phenolic content and showed the most potent activity against DPPH radical. All extracts exhibited complete protection against the damage induced by hydrogen peroxide (H(2)O(2)) by increasing cell viability compared to only H(2)O(2)-treated groups, both in co-treatment and pre-treatment protocols. None of the extracts exhibited cytotoxic activity, and most of the extracts from the TS stimulated fibroblast proliferation and migration. All TS extracts exert anti-inflammatory activity by suppressing the overproduction of tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO). The most pronounced activity on hydroxyproline synthesis was observed in D extract. In summary, it was observed that TS extracts can promote the healing process by enhancing fibroblast migration, proliferation and collagen synthesis as well as suppressing pro-inflammatory cytokines. The obtained data in this work support the traditional use of TS as a valuable plant-based compound for the treatment of wounds. MDPI 2019-09-14 /pmc/articles/PMC6767006/ /pubmed/31540139 http://dx.doi.org/10.3390/molecules24183353 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ustuner, Oya
Anlas, Ceren
Bakirel, Tulay
Ustun-Alkan, Fulya
Diren Sigirci, Belgi
Ak, Seyyal
Akpulat, Huseyin Askin
Donmez, Ceylan
Koca-Caliskan, Ufuk
In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas
title In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas
title_full In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas
title_fullStr In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas
title_full_unstemmed In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas
title_short In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas
title_sort in vitro evaluation of antioxidant, anti-inflammatory, antimicrobial and wound healing potential of thymus sipyleus boiss. subsp. rosulans (borbas) jalas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767006/
https://www.ncbi.nlm.nih.gov/pubmed/31540139
http://dx.doi.org/10.3390/molecules24183353
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