Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator

A new cyan fluorescent probe, MIPY-DNBS, using an imidazo[1,5-α]pyridine derivative as the fluorophore and 2,4-dinitrobenzensufonate as the recognition site for the selective detection of thiols (Cys, GSH, and Hcy), was designed and synthesized. Probe MIPY-DNBS exhibited a 172 nm Stokes shift, a fas...

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Detalles Bibliográficos
Autores principales: Chen, Song, Hou, Peng, Sun, Jingwen, Wang, Haijun, Liu, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767008/
https://www.ncbi.nlm.nih.gov/pubmed/31547406
http://dx.doi.org/10.3390/molecules24183328
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author Chen, Song
Hou, Peng
Sun, Jingwen
Wang, Haijun
Liu, Lei
author_facet Chen, Song
Hou, Peng
Sun, Jingwen
Wang, Haijun
Liu, Lei
author_sort Chen, Song
collection PubMed
description A new cyan fluorescent probe, MIPY-DNBS, using an imidazo[1,5-α]pyridine derivative as the fluorophore and 2,4-dinitrobenzensufonate as the recognition site for the selective detection of thiols (Cys, GSH, and Hcy), was designed and synthesized. Probe MIPY-DNBS exhibited a 172 nm Stokes shift, a fast response time (400 s), low cytotoxicity, low detection limits (12.7 nM for Cys), and excellent selectively in the detection of thiols. In addition, MIPY-DNBS was successfully applied to imaging thiols in living MCF-7 cells and zebrafish.
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spelling pubmed-67670082019-10-02 Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator Chen, Song Hou, Peng Sun, Jingwen Wang, Haijun Liu, Lei Molecules Article A new cyan fluorescent probe, MIPY-DNBS, using an imidazo[1,5-α]pyridine derivative as the fluorophore and 2,4-dinitrobenzensufonate as the recognition site for the selective detection of thiols (Cys, GSH, and Hcy), was designed and synthesized. Probe MIPY-DNBS exhibited a 172 nm Stokes shift, a fast response time (400 s), low cytotoxicity, low detection limits (12.7 nM for Cys), and excellent selectively in the detection of thiols. In addition, MIPY-DNBS was successfully applied to imaging thiols in living MCF-7 cells and zebrafish. MDPI 2019-09-12 /pmc/articles/PMC6767008/ /pubmed/31547406 http://dx.doi.org/10.3390/molecules24183328 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Song
Hou, Peng
Sun, Jingwen
Wang, Haijun
Liu, Lei
Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator
title Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator
title_full Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator
title_fullStr Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator
title_full_unstemmed Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator
title_short Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator
title_sort recognition of thiols in living cells and zebrafish using an imidazo[1,5-α]pyridine-derivative indicator
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767008/
https://www.ncbi.nlm.nih.gov/pubmed/31547406
http://dx.doi.org/10.3390/molecules24183328
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