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GATA‐1 isoforms differently contribute to the production and compartmentation of reactive oxygen species in the myeloid leukemia cell line K562
Maintenance of a balanced expression of the two isoforms of the transcription factor GATA‐1, the full‐length protein (GATA‐1(FL)) and a shorter isoform (GATA‐1 (S)), contributes to control hematopoiesis, whereas their dysregulation can alter the differentiation/proliferation potential of hematopoiet...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767011/ https://www.ncbi.nlm.nih.gov/pubmed/31049966 http://dx.doi.org/10.1002/jcp.28688 |
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author | Riccio, Patrizia Sessa, Raffaele de Nicola, Sergio Petruzziello, Fara Trombetti, Silvia Menna, Giuseppe Pepe, Giampiero Maddalena, Pasquale Izzo, Paola Grosso, Michela |
author_facet | Riccio, Patrizia Sessa, Raffaele de Nicola, Sergio Petruzziello, Fara Trombetti, Silvia Menna, Giuseppe Pepe, Giampiero Maddalena, Pasquale Izzo, Paola Grosso, Michela |
author_sort | Riccio, Patrizia |
collection | PubMed |
description | Maintenance of a balanced expression of the two isoforms of the transcription factor GATA‐1, the full‐length protein (GATA‐1(FL)) and a shorter isoform (GATA‐1 (S)), contributes to control hematopoiesis, whereas their dysregulation can alter the differentiation/proliferation potential of hematopoietic precursors thereby eventually leading to a variety of hematopoietic disorders. Although it is well established that these isoforms play opposite roles in these remarkable processes, most of the molecular pathways involved remain unknown. Here, we demonstrate that GATA‐1(FL) and GATA‐1(S) are able to differently influence intracellular redox states and reactive oxygen species (ROS) compartmentation in the erythroleukemic K562 cell line, thus shedding novel mechanistic insights into the processes of cell proliferation and apoptosis resistance in myeloid precursors. Furthermore, given the role played by ROS signaling as a strategy to escape apoptosis and evade cell‐mediated immunity in myeloid cells, this study highlights a mechanism through which aberrant expression of GATA‐1 isoforms could play a role in the leukemogenic process. |
format | Online Article Text |
id | pubmed-6767011 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67670112019-10-01 GATA‐1 isoforms differently contribute to the production and compartmentation of reactive oxygen species in the myeloid leukemia cell line K562 Riccio, Patrizia Sessa, Raffaele de Nicola, Sergio Petruzziello, Fara Trombetti, Silvia Menna, Giuseppe Pepe, Giampiero Maddalena, Pasquale Izzo, Paola Grosso, Michela J Cell Physiol Original Research Articles Maintenance of a balanced expression of the two isoforms of the transcription factor GATA‐1, the full‐length protein (GATA‐1(FL)) and a shorter isoform (GATA‐1 (S)), contributes to control hematopoiesis, whereas their dysregulation can alter the differentiation/proliferation potential of hematopoietic precursors thereby eventually leading to a variety of hematopoietic disorders. Although it is well established that these isoforms play opposite roles in these remarkable processes, most of the molecular pathways involved remain unknown. Here, we demonstrate that GATA‐1(FL) and GATA‐1(S) are able to differently influence intracellular redox states and reactive oxygen species (ROS) compartmentation in the erythroleukemic K562 cell line, thus shedding novel mechanistic insights into the processes of cell proliferation and apoptosis resistance in myeloid precursors. Furthermore, given the role played by ROS signaling as a strategy to escape apoptosis and evade cell‐mediated immunity in myeloid cells, this study highlights a mechanism through which aberrant expression of GATA‐1 isoforms could play a role in the leukemogenic process. John Wiley and Sons Inc. 2019-05-02 2019-11 /pmc/articles/PMC6767011/ /pubmed/31049966 http://dx.doi.org/10.1002/jcp.28688 Text en © 2019 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Original Research Articles Riccio, Patrizia Sessa, Raffaele de Nicola, Sergio Petruzziello, Fara Trombetti, Silvia Menna, Giuseppe Pepe, Giampiero Maddalena, Pasquale Izzo, Paola Grosso, Michela GATA‐1 isoforms differently contribute to the production and compartmentation of reactive oxygen species in the myeloid leukemia cell line K562 |
title | GATA‐1 isoforms differently contribute to the production and compartmentation of reactive oxygen species in the myeloid leukemia cell line K562 |
title_full | GATA‐1 isoforms differently contribute to the production and compartmentation of reactive oxygen species in the myeloid leukemia cell line K562 |
title_fullStr | GATA‐1 isoforms differently contribute to the production and compartmentation of reactive oxygen species in the myeloid leukemia cell line K562 |
title_full_unstemmed | GATA‐1 isoforms differently contribute to the production and compartmentation of reactive oxygen species in the myeloid leukemia cell line K562 |
title_short | GATA‐1 isoforms differently contribute to the production and compartmentation of reactive oxygen species in the myeloid leukemia cell line K562 |
title_sort | gata‐1 isoforms differently contribute to the production and compartmentation of reactive oxygen species in the myeloid leukemia cell line k562 |
topic | Original Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767011/ https://www.ncbi.nlm.nih.gov/pubmed/31049966 http://dx.doi.org/10.1002/jcp.28688 |
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