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Castanea sativa Mill. Shells Aqueous Extract Exhibits Anticancer Properties Inducing Cytotoxic and Pro-Apoptotic Effects

In this study, chestnut shells (CS) were used in order to obtain bioactive compounds through different extraction procedures. The aqueous extracts were chemically characterized. The highest extraction yield and total phenolic content was obtained by conventional liquid extraction (CLE). Gallic and p...

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Autores principales: Cacciola, Nunzio Antonio, Squillaci, Giuseppe, D’Apolito, Mariella, Petillo, Orsolina, Veraldi, Francesco, La Cara, Francesco, Peluso, Gianfranco, Margarucci, Sabrina, Morana, Alessandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767178/
https://www.ncbi.nlm.nih.gov/pubmed/31546790
http://dx.doi.org/10.3390/molecules24183401
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author Cacciola, Nunzio Antonio
Squillaci, Giuseppe
D’Apolito, Mariella
Petillo, Orsolina
Veraldi, Francesco
La Cara, Francesco
Peluso, Gianfranco
Margarucci, Sabrina
Morana, Alessandra
author_facet Cacciola, Nunzio Antonio
Squillaci, Giuseppe
D’Apolito, Mariella
Petillo, Orsolina
Veraldi, Francesco
La Cara, Francesco
Peluso, Gianfranco
Margarucci, Sabrina
Morana, Alessandra
author_sort Cacciola, Nunzio Antonio
collection PubMed
description In this study, chestnut shells (CS) were used in order to obtain bioactive compounds through different extraction procedures. The aqueous extracts were chemically characterized. The highest extraction yield and total phenolic content was obtained by conventional liquid extraction (CLE). Gallic and protocatechuic acids were the main simple phenols in the extract, with 86.97 and 11.20 mg/g chestnut shells dry extract (CSDE), respectively. Six tumor cell lines (DU 145, PC-3, LNCaP, MDA-MB-231, MCF-7, and HepG2) and one normal prostate epithelial cell line (PNT2) were exposed to increasing concentration of CSDE (1–100 µg/mL) for 24 h, and cell viability was evaluated using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide MTT assay. A reduced rate in cell viability was observed in DU 145, PC-3, LNCaP, and MCF-7 cells, while viability of the other assessed cells was not affected, except for PNT2 cells at a concentration of 100 μg/mL. Furthermore, CSDE—at concentrations of 55.5 and 100 µg/mL—lead to a significant increase of apoptotic cells in DU 145 cells of 28.2% and 61%, respectively. In conclusion, these outcomes suggested that CS might be used for the extraction of several polyphenols that may represent good candidates for alternative therapies or in combination with current chemotherapeutics.
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spelling pubmed-67671782019-10-02 Castanea sativa Mill. Shells Aqueous Extract Exhibits Anticancer Properties Inducing Cytotoxic and Pro-Apoptotic Effects Cacciola, Nunzio Antonio Squillaci, Giuseppe D’Apolito, Mariella Petillo, Orsolina Veraldi, Francesco La Cara, Francesco Peluso, Gianfranco Margarucci, Sabrina Morana, Alessandra Molecules Article In this study, chestnut shells (CS) were used in order to obtain bioactive compounds through different extraction procedures. The aqueous extracts were chemically characterized. The highest extraction yield and total phenolic content was obtained by conventional liquid extraction (CLE). Gallic and protocatechuic acids were the main simple phenols in the extract, with 86.97 and 11.20 mg/g chestnut shells dry extract (CSDE), respectively. Six tumor cell lines (DU 145, PC-3, LNCaP, MDA-MB-231, MCF-7, and HepG2) and one normal prostate epithelial cell line (PNT2) were exposed to increasing concentration of CSDE (1–100 µg/mL) for 24 h, and cell viability was evaluated using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide MTT assay. A reduced rate in cell viability was observed in DU 145, PC-3, LNCaP, and MCF-7 cells, while viability of the other assessed cells was not affected, except for PNT2 cells at a concentration of 100 μg/mL. Furthermore, CSDE—at concentrations of 55.5 and 100 µg/mL—lead to a significant increase of apoptotic cells in DU 145 cells of 28.2% and 61%, respectively. In conclusion, these outcomes suggested that CS might be used for the extraction of several polyphenols that may represent good candidates for alternative therapies or in combination with current chemotherapeutics. MDPI 2019-09-19 /pmc/articles/PMC6767178/ /pubmed/31546790 http://dx.doi.org/10.3390/molecules24183401 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cacciola, Nunzio Antonio
Squillaci, Giuseppe
D’Apolito, Mariella
Petillo, Orsolina
Veraldi, Francesco
La Cara, Francesco
Peluso, Gianfranco
Margarucci, Sabrina
Morana, Alessandra
Castanea sativa Mill. Shells Aqueous Extract Exhibits Anticancer Properties Inducing Cytotoxic and Pro-Apoptotic Effects
title Castanea sativa Mill. Shells Aqueous Extract Exhibits Anticancer Properties Inducing Cytotoxic and Pro-Apoptotic Effects
title_full Castanea sativa Mill. Shells Aqueous Extract Exhibits Anticancer Properties Inducing Cytotoxic and Pro-Apoptotic Effects
title_fullStr Castanea sativa Mill. Shells Aqueous Extract Exhibits Anticancer Properties Inducing Cytotoxic and Pro-Apoptotic Effects
title_full_unstemmed Castanea sativa Mill. Shells Aqueous Extract Exhibits Anticancer Properties Inducing Cytotoxic and Pro-Apoptotic Effects
title_short Castanea sativa Mill. Shells Aqueous Extract Exhibits Anticancer Properties Inducing Cytotoxic and Pro-Apoptotic Effects
title_sort castanea sativa mill. shells aqueous extract exhibits anticancer properties inducing cytotoxic and pro-apoptotic effects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767178/
https://www.ncbi.nlm.nih.gov/pubmed/31546790
http://dx.doi.org/10.3390/molecules24183401
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