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Neutral Lipid Content in Lipid Droplets: Potential Biomarker of Cordycepin Accumulation in Cordycepin-Producing Fungi

To clarify the relationship between neutral lipid content and cordycepin accumulation in Cordyceps militaris, mutants were generated from mixed spores of two C. militaris strains with varying cordycepin-producing capacities. Fifteen stable mutants producing from 0.001 to 2.363 mg/mL cordycepin were...

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Autores principales: Qin, Peng, Wang, ZhiYe, Lu, DengXue, Kang, HongMei, Li, Guang, Guo, Rui, Zhao, YuHui, Han, RongBing, Ji, Bing, Zeng, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767356/
https://www.ncbi.nlm.nih.gov/pubmed/31527427
http://dx.doi.org/10.3390/molecules24183363
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author Qin, Peng
Wang, ZhiYe
Lu, DengXue
Kang, HongMei
Li, Guang
Guo, Rui
Zhao, YuHui
Han, RongBing
Ji, Bing
Zeng, Yang
author_facet Qin, Peng
Wang, ZhiYe
Lu, DengXue
Kang, HongMei
Li, Guang
Guo, Rui
Zhao, YuHui
Han, RongBing
Ji, Bing
Zeng, Yang
author_sort Qin, Peng
collection PubMed
description To clarify the relationship between neutral lipid content and cordycepin accumulation in Cordyceps militaris, mutants were generated from mixed spores of two C. militaris strains with varying cordycepin-producing capacities. Fifteen stable mutants producing from 0.001 to 2.363 mg/mL cordycepin were finally selected. The relative fluorescence intensities of the 15 mutants, two C. militaris strains and an Aspergillus nidulans strain at different concentrations of lyophilized mycelium powder were then investigated using the Nile red method. The mutant CM1-1-1 with the highest relative fluorescence intensity among the eighteen strains was selected for optimizing the Nile red method. Relative fluorescence intensity was linearly correlated with cordycepin concentration in liquid broth (R(2) = 0.9514) and in lyophilized mycelium powder (R(2) = 0.9378) for the 18 cordycepin-producing strains under identical culture conditions and with cordycepin concentration in liquid broth (R(2) = 0.9727) and in lyophilized mycelium powder (R(2) = 0.9613) for CM1-1-1 under eight different sets of conditions. In addition, the cordycepin content in lyophilized mycelium powder measured by the Nile red method was linearly correlated with that determined by an HPLC method (R(2) = 0.9627). In conclusion, neutral lipids in lipid droplets are required during cordycepin accumulation; these neutral lipids are potential biomarkers of cordycepin biosynthesis.
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spelling pubmed-67673562019-10-02 Neutral Lipid Content in Lipid Droplets: Potential Biomarker of Cordycepin Accumulation in Cordycepin-Producing Fungi Qin, Peng Wang, ZhiYe Lu, DengXue Kang, HongMei Li, Guang Guo, Rui Zhao, YuHui Han, RongBing Ji, Bing Zeng, Yang Molecules Article To clarify the relationship between neutral lipid content and cordycepin accumulation in Cordyceps militaris, mutants were generated from mixed spores of two C. militaris strains with varying cordycepin-producing capacities. Fifteen stable mutants producing from 0.001 to 2.363 mg/mL cordycepin were finally selected. The relative fluorescence intensities of the 15 mutants, two C. militaris strains and an Aspergillus nidulans strain at different concentrations of lyophilized mycelium powder were then investigated using the Nile red method. The mutant CM1-1-1 with the highest relative fluorescence intensity among the eighteen strains was selected for optimizing the Nile red method. Relative fluorescence intensity was linearly correlated with cordycepin concentration in liquid broth (R(2) = 0.9514) and in lyophilized mycelium powder (R(2) = 0.9378) for the 18 cordycepin-producing strains under identical culture conditions and with cordycepin concentration in liquid broth (R(2) = 0.9727) and in lyophilized mycelium powder (R(2) = 0.9613) for CM1-1-1 under eight different sets of conditions. In addition, the cordycepin content in lyophilized mycelium powder measured by the Nile red method was linearly correlated with that determined by an HPLC method (R(2) = 0.9627). In conclusion, neutral lipids in lipid droplets are required during cordycepin accumulation; these neutral lipids are potential biomarkers of cordycepin biosynthesis. MDPI 2019-09-16 /pmc/articles/PMC6767356/ /pubmed/31527427 http://dx.doi.org/10.3390/molecules24183363 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Qin, Peng
Wang, ZhiYe
Lu, DengXue
Kang, HongMei
Li, Guang
Guo, Rui
Zhao, YuHui
Han, RongBing
Ji, Bing
Zeng, Yang
Neutral Lipid Content in Lipid Droplets: Potential Biomarker of Cordycepin Accumulation in Cordycepin-Producing Fungi
title Neutral Lipid Content in Lipid Droplets: Potential Biomarker of Cordycepin Accumulation in Cordycepin-Producing Fungi
title_full Neutral Lipid Content in Lipid Droplets: Potential Biomarker of Cordycepin Accumulation in Cordycepin-Producing Fungi
title_fullStr Neutral Lipid Content in Lipid Droplets: Potential Biomarker of Cordycepin Accumulation in Cordycepin-Producing Fungi
title_full_unstemmed Neutral Lipid Content in Lipid Droplets: Potential Biomarker of Cordycepin Accumulation in Cordycepin-Producing Fungi
title_short Neutral Lipid Content in Lipid Droplets: Potential Biomarker of Cordycepin Accumulation in Cordycepin-Producing Fungi
title_sort neutral lipid content in lipid droplets: potential biomarker of cordycepin accumulation in cordycepin-producing fungi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767356/
https://www.ncbi.nlm.nih.gov/pubmed/31527427
http://dx.doi.org/10.3390/molecules24183363
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