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Measuring biomaterials mechanics with atomic force microscopy. 1. Influence of the loading rate and applied force (pyramidal tips)

Atomic force microscopy (AFM) is today an established tool in imaging and determination of mechanical properties of biomaterials. Due to their complex organization, those materials show intricate properties such as viscoelasticity. Therefore, one has to consider that the loading rate at which the sa...

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Detalles Bibliográficos
Autores principales: Weber, Andreas, Iturri, Jagoba, Benitez, Rafael, Toca‐Herrera, José L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767567/
https://www.ncbi.nlm.nih.gov/pubmed/31106928
http://dx.doi.org/10.1002/jemt.23291
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author Weber, Andreas
Iturri, Jagoba
Benitez, Rafael
Toca‐Herrera, José L.
author_facet Weber, Andreas
Iturri, Jagoba
Benitez, Rafael
Toca‐Herrera, José L.
author_sort Weber, Andreas
collection PubMed
description Atomic force microscopy (AFM) is today an established tool in imaging and determination of mechanical properties of biomaterials. Due to their complex organization, those materials show intricate properties such as viscoelasticity. Therefore, one has to consider that the loading rate at which the sample is probed will lead to different mechanical response (properties). In this work, we studied the dependence of the mechanical properties of endothelial cells on the loading rate using AFM in force spectroscopy mode. We employed a sharp, four‐sided pyramidal indenter and loading rates ranging from 0.5 to 20 μm/s. In addition, by variation of the load (applied forces from 100 to 10,000 pN), the dependence of the cell properties on indentation depth could be determined. We then showed that the mechanical response of endothelial cells depends nonlinearly on the loading rate and follows a weak power‐law. In addition, regions of different viscous response at varying indentation depth could be determined. Based on the results we obtained, a general route map for AFM users for design of cell mechanics experiments was described.
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spelling pubmed-67675672019-10-03 Measuring biomaterials mechanics with atomic force microscopy. 1. Influence of the loading rate and applied force (pyramidal tips) Weber, Andreas Iturri, Jagoba Benitez, Rafael Toca‐Herrera, José L. Microsc Res Tech Research Articles Atomic force microscopy (AFM) is today an established tool in imaging and determination of mechanical properties of biomaterials. Due to their complex organization, those materials show intricate properties such as viscoelasticity. Therefore, one has to consider that the loading rate at which the sample is probed will lead to different mechanical response (properties). In this work, we studied the dependence of the mechanical properties of endothelial cells on the loading rate using AFM in force spectroscopy mode. We employed a sharp, four‐sided pyramidal indenter and loading rates ranging from 0.5 to 20 μm/s. In addition, by variation of the load (applied forces from 100 to 10,000 pN), the dependence of the cell properties on indentation depth could be determined. We then showed that the mechanical response of endothelial cells depends nonlinearly on the loading rate and follows a weak power‐law. In addition, regions of different viscous response at varying indentation depth could be determined. Based on the results we obtained, a general route map for AFM users for design of cell mechanics experiments was described. John Wiley & Sons, Inc. 2019-05-20 2019-09 /pmc/articles/PMC6767567/ /pubmed/31106928 http://dx.doi.org/10.1002/jemt.23291 Text en © 2019 The Authors. Microscopy Research and Technique Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Weber, Andreas
Iturri, Jagoba
Benitez, Rafael
Toca‐Herrera, José L.
Measuring biomaterials mechanics with atomic force microscopy. 1. Influence of the loading rate and applied force (pyramidal tips)
title Measuring biomaterials mechanics with atomic force microscopy. 1. Influence of the loading rate and applied force (pyramidal tips)
title_full Measuring biomaterials mechanics with atomic force microscopy. 1. Influence of the loading rate and applied force (pyramidal tips)
title_fullStr Measuring biomaterials mechanics with atomic force microscopy. 1. Influence of the loading rate and applied force (pyramidal tips)
title_full_unstemmed Measuring biomaterials mechanics with atomic force microscopy. 1. Influence of the loading rate and applied force (pyramidal tips)
title_short Measuring biomaterials mechanics with atomic force microscopy. 1. Influence of the loading rate and applied force (pyramidal tips)
title_sort measuring biomaterials mechanics with atomic force microscopy. 1. influence of the loading rate and applied force (pyramidal tips)
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767567/
https://www.ncbi.nlm.nih.gov/pubmed/31106928
http://dx.doi.org/10.1002/jemt.23291
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