Synergistic effect of a retinoid X receptor-selective ligand bexarotene and docetaxel in prostate cancer

PURPOSE: To explore if bexarotene (BEX) synergistically enhances docetaxel (DTX) cytotoxicity in castration-resistant prostate cancer cell lines. MATERIALS AND METHODS: MTT assay was used to measure the cytotoxic effect of DTX and BEX on castration-resistant prostate cancer (CRPC) cell proliferation...

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Autores principales: Shen, Danyang, Wang, Huan, Zheng, Qiming, Cheng, Sheng, Xu, Liwei, Wang, Mingchao, Li, Gong H, Xia, Li Q
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768013/
https://www.ncbi.nlm.nih.gov/pubmed/31576145
http://dx.doi.org/10.2147/OTT.S209307
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author Shen, Danyang
Wang, Huan
Zheng, Qiming
Cheng, Sheng
Xu, Liwei
Wang, Mingchao
Li, Gong H
Xia, Li Q
author_facet Shen, Danyang
Wang, Huan
Zheng, Qiming
Cheng, Sheng
Xu, Liwei
Wang, Mingchao
Li, Gong H
Xia, Li Q
author_sort Shen, Danyang
collection PubMed
description PURPOSE: To explore if bexarotene (BEX) synergistically enhances docetaxel (DTX) cytotoxicity in castration-resistant prostate cancer cell lines. MATERIALS AND METHODS: MTT assay was used to measure the cytotoxic effect of DTX and BEX on castration-resistant prostate cancer (CRPC) cell proliferation and the combination index (CI) values calculated to analyze the interaction between DTX and BEX. Flow cytometry and Western blot analysis identified the underlying mechanism for the synergistic effect of BEX and DTX. RESULTS: When mitotic slippage happens, BEX can synergistically strengthen the anti-proliferation of DTX in a way of significantly down-regulating cyclinB1 and CDK1 expression, and then arresting cells in G2 phase. CONCLUSION: Results from this study showed that BEX-induced G2 arrest and DTX-induced mitotic arrest probably contributed to the synergistic effect of BEX and DTX.
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spelling pubmed-67680132019-10-01 Synergistic effect of a retinoid X receptor-selective ligand bexarotene and docetaxel in prostate cancer Shen, Danyang Wang, Huan Zheng, Qiming Cheng, Sheng Xu, Liwei Wang, Mingchao Li, Gong H Xia, Li Q Onco Targets Ther Original Research PURPOSE: To explore if bexarotene (BEX) synergistically enhances docetaxel (DTX) cytotoxicity in castration-resistant prostate cancer cell lines. MATERIALS AND METHODS: MTT assay was used to measure the cytotoxic effect of DTX and BEX on castration-resistant prostate cancer (CRPC) cell proliferation and the combination index (CI) values calculated to analyze the interaction between DTX and BEX. Flow cytometry and Western blot analysis identified the underlying mechanism for the synergistic effect of BEX and DTX. RESULTS: When mitotic slippage happens, BEX can synergistically strengthen the anti-proliferation of DTX in a way of significantly down-regulating cyclinB1 and CDK1 expression, and then arresting cells in G2 phase. CONCLUSION: Results from this study showed that BEX-induced G2 arrest and DTX-induced mitotic arrest probably contributed to the synergistic effect of BEX and DTX. Dove 2019-09-24 /pmc/articles/PMC6768013/ /pubmed/31576145 http://dx.doi.org/10.2147/OTT.S209307 Text en © 2019 Shen et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Shen, Danyang
Wang, Huan
Zheng, Qiming
Cheng, Sheng
Xu, Liwei
Wang, Mingchao
Li, Gong H
Xia, Li Q
Synergistic effect of a retinoid X receptor-selective ligand bexarotene and docetaxel in prostate cancer
title Synergistic effect of a retinoid X receptor-selective ligand bexarotene and docetaxel in prostate cancer
title_full Synergistic effect of a retinoid X receptor-selective ligand bexarotene and docetaxel in prostate cancer
title_fullStr Synergistic effect of a retinoid X receptor-selective ligand bexarotene and docetaxel in prostate cancer
title_full_unstemmed Synergistic effect of a retinoid X receptor-selective ligand bexarotene and docetaxel in prostate cancer
title_short Synergistic effect of a retinoid X receptor-selective ligand bexarotene and docetaxel in prostate cancer
title_sort synergistic effect of a retinoid x receptor-selective ligand bexarotene and docetaxel in prostate cancer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768013/
https://www.ncbi.nlm.nih.gov/pubmed/31576145
http://dx.doi.org/10.2147/OTT.S209307
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