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LysR-type transcriptional regulator OvrB encoded in O island 9 drives enterohemorrhagic Escherichia coli O157:H7 virulence

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 (O157) is a major foodborne pathogen that causes severe illness in humans worldwide. The genome of O157 contains 177 genomic islands known as O islands (OIs), including Shiga toxin-converting phages (OI-45 and OI-93) and the locus for enterocyte effa...

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Autores principales: Liu, Yutao, Liu, Bin, Yang, Pan, Wang, Ting, Chang, Zhanhe, Wang, Junyue, Wang, Qian, Li, Wendi, Wu, Jialin, Huang, Di, Jiang, Lingyan, Yang, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768210/
https://www.ncbi.nlm.nih.gov/pubmed/31502495
http://dx.doi.org/10.1080/21505594.2019.1661721
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author Liu, Yutao
Liu, Bin
Yang, Pan
Wang, Ting
Chang, Zhanhe
Wang, Junyue
Wang, Qian
Li, Wendi
Wu, Jialin
Huang, Di
Jiang, Lingyan
Yang, Bin
author_facet Liu, Yutao
Liu, Bin
Yang, Pan
Wang, Ting
Chang, Zhanhe
Wang, Junyue
Wang, Qian
Li, Wendi
Wu, Jialin
Huang, Di
Jiang, Lingyan
Yang, Bin
author_sort Liu, Yutao
collection PubMed
description Enterohemorrhagic Escherichia coli (EHEC) O157:H7 (O157) is a major foodborne pathogen that causes severe illness in humans worldwide. The genome of O157 contains 177 genomic islands known as O islands (OIs), including Shiga toxin-converting phages (OI-45 and OI-93) and the locus for enterocyte effacement (LEE) pathogenicity island (OI-148). However, most genes in OIs are uncharacterized and code for unknown functions. In this study, we demonstrated, for the first time, that OI-9 encodes a novel transcriptional activator, Z0346 (named OvrB), which is required for bacterial adherence to host cells and LEE gene expression in O157. OvrB directly binds to the promoter region of LEE1 and activates the transcription of ler (encoding a master regulator of LEE genes), which in turn activates LEE1–5 genes to promote O157 adherence. Furthermore, mouse oral infection assays showed that OvrB promotes O157 colonization in the mouse intestine. Finally, OvrB is shown to be a widespread transcriptional activator of virulence genes in other enterohemorrhagic and enteropathogenic Escherichia coli serotypes. Our work significantly expands the understanding of bacterial virulence control and provides new evidence suggesting that horizontally transferred regulator genes mediate LEE gene expression.
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spelling pubmed-67682102019-10-09 LysR-type transcriptional regulator OvrB encoded in O island 9 drives enterohemorrhagic Escherichia coli O157:H7 virulence Liu, Yutao Liu, Bin Yang, Pan Wang, Ting Chang, Zhanhe Wang, Junyue Wang, Qian Li, Wendi Wu, Jialin Huang, Di Jiang, Lingyan Yang, Bin Virulence Research Paper Enterohemorrhagic Escherichia coli (EHEC) O157:H7 (O157) is a major foodborne pathogen that causes severe illness in humans worldwide. The genome of O157 contains 177 genomic islands known as O islands (OIs), including Shiga toxin-converting phages (OI-45 and OI-93) and the locus for enterocyte effacement (LEE) pathogenicity island (OI-148). However, most genes in OIs are uncharacterized and code for unknown functions. In this study, we demonstrated, for the first time, that OI-9 encodes a novel transcriptional activator, Z0346 (named OvrB), which is required for bacterial adherence to host cells and LEE gene expression in O157. OvrB directly binds to the promoter region of LEE1 and activates the transcription of ler (encoding a master regulator of LEE genes), which in turn activates LEE1–5 genes to promote O157 adherence. Furthermore, mouse oral infection assays showed that OvrB promotes O157 colonization in the mouse intestine. Finally, OvrB is shown to be a widespread transcriptional activator of virulence genes in other enterohemorrhagic and enteropathogenic Escherichia coli serotypes. Our work significantly expands the understanding of bacterial virulence control and provides new evidence suggesting that horizontally transferred regulator genes mediate LEE gene expression. Taylor & Francis 2019-09-10 /pmc/articles/PMC6768210/ /pubmed/31502495 http://dx.doi.org/10.1080/21505594.2019.1661721 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Liu, Yutao
Liu, Bin
Yang, Pan
Wang, Ting
Chang, Zhanhe
Wang, Junyue
Wang, Qian
Li, Wendi
Wu, Jialin
Huang, Di
Jiang, Lingyan
Yang, Bin
LysR-type transcriptional regulator OvrB encoded in O island 9 drives enterohemorrhagic Escherichia coli O157:H7 virulence
title LysR-type transcriptional regulator OvrB encoded in O island 9 drives enterohemorrhagic Escherichia coli O157:H7 virulence
title_full LysR-type transcriptional regulator OvrB encoded in O island 9 drives enterohemorrhagic Escherichia coli O157:H7 virulence
title_fullStr LysR-type transcriptional regulator OvrB encoded in O island 9 drives enterohemorrhagic Escherichia coli O157:H7 virulence
title_full_unstemmed LysR-type transcriptional regulator OvrB encoded in O island 9 drives enterohemorrhagic Escherichia coli O157:H7 virulence
title_short LysR-type transcriptional regulator OvrB encoded in O island 9 drives enterohemorrhagic Escherichia coli O157:H7 virulence
title_sort lysr-type transcriptional regulator ovrb encoded in o island 9 drives enterohemorrhagic escherichia coli o157:h7 virulence
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768210/
https://www.ncbi.nlm.nih.gov/pubmed/31502495
http://dx.doi.org/10.1080/21505594.2019.1661721
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