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Fibroblast feeder layer supports adipogenic differentiation of human adipose stromal/progenitor cells

Adipose stromal/progenitor cells (ASCs) can differentiate into adipocytes in the course of adipogenesis. This process is governed by systemic factors and signals of the adipose stem cell niche. ASCs isolated from fat tissues and amplified in vitro provide an essential and reliable model system to st...

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Autores principales: Ejaz, Asim, Hatzmann, Florian M, Hammerle, Sarina, Ritthammer, Heike, Mattesich, Monika, Zwierzina, Marit, Waldegger, Petra, Zwerschke, Werner
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768258/
https://www.ncbi.nlm.nih.gov/pubmed/31033380
http://dx.doi.org/10.1080/21623945.2019.1608751
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author Ejaz, Asim
Hatzmann, Florian M
Hammerle, Sarina
Ritthammer, Heike
Mattesich, Monika
Zwierzina, Marit
Waldegger, Petra
Zwerschke, Werner
author_facet Ejaz, Asim
Hatzmann, Florian M
Hammerle, Sarina
Ritthammer, Heike
Mattesich, Monika
Zwierzina, Marit
Waldegger, Petra
Zwerschke, Werner
author_sort Ejaz, Asim
collection PubMed
description Adipose stromal/progenitor cells (ASCs) can differentiate into adipocytes in the course of adipogenesis. This process is governed by systemic factors and signals of the adipose stem cell niche. ASCs isolated from fat tissues and amplified in vitro provide an essential and reliable model system to study adipogenesis. However, current cell culture models routinely grow ASCs on plastic surfaces largely missing niche parameters. In the present communication, we employed human foreskin fibroblasts (HFFs) monolayers as feeder cells for ASCs, which were isolated from human subcutaneous white adipose tissue and amplified in vitro. We found that PPARγ2 and several adipocyte markers were significantly higher expressed in differentiated ASCs growing on feeder layers relative to plastic dishes. Moreover, a significant higher number of adipocytes was generated from ASCs cultured on feeder layer and these adipocytes contained larger fat droplets. Insulin strongly stimulated glucose uptake into adipocytes produced on feeder layer suggesting that these cells show characteristic metabolic features of fat cells.  Finally, we show that the HFF feeder layer allows adipogenic differentiation of low-density-seeded ASCs. In conclusion, we demonstrate that the HFF feeder layer increases adipocyte differentiation of ASCs and allows differentiation of low density seeded progenitor cells  into functional adipocytes.
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spelling pubmed-67682582019-10-09 Fibroblast feeder layer supports adipogenic differentiation of human adipose stromal/progenitor cells Ejaz, Asim Hatzmann, Florian M Hammerle, Sarina Ritthammer, Heike Mattesich, Monika Zwierzina, Marit Waldegger, Petra Zwerschke, Werner Adipocyte Brief Report Adipose stromal/progenitor cells (ASCs) can differentiate into adipocytes in the course of adipogenesis. This process is governed by systemic factors and signals of the adipose stem cell niche. ASCs isolated from fat tissues and amplified in vitro provide an essential and reliable model system to study adipogenesis. However, current cell culture models routinely grow ASCs on plastic surfaces largely missing niche parameters. In the present communication, we employed human foreskin fibroblasts (HFFs) monolayers as feeder cells for ASCs, which were isolated from human subcutaneous white adipose tissue and amplified in vitro. We found that PPARγ2 and several adipocyte markers were significantly higher expressed in differentiated ASCs growing on feeder layers relative to plastic dishes. Moreover, a significant higher number of adipocytes was generated from ASCs cultured on feeder layer and these adipocytes contained larger fat droplets. Insulin strongly stimulated glucose uptake into adipocytes produced on feeder layer suggesting that these cells show characteristic metabolic features of fat cells.  Finally, we show that the HFF feeder layer allows adipogenic differentiation of low-density-seeded ASCs. In conclusion, we demonstrate that the HFF feeder layer increases adipocyte differentiation of ASCs and allows differentiation of low density seeded progenitor cells  into functional adipocytes. Taylor & Francis 2019-04-29 /pmc/articles/PMC6768258/ /pubmed/31033380 http://dx.doi.org/10.1080/21623945.2019.1608751 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Report
Ejaz, Asim
Hatzmann, Florian M
Hammerle, Sarina
Ritthammer, Heike
Mattesich, Monika
Zwierzina, Marit
Waldegger, Petra
Zwerschke, Werner
Fibroblast feeder layer supports adipogenic differentiation of human adipose stromal/progenitor cells
title Fibroblast feeder layer supports adipogenic differentiation of human adipose stromal/progenitor cells
title_full Fibroblast feeder layer supports adipogenic differentiation of human adipose stromal/progenitor cells
title_fullStr Fibroblast feeder layer supports adipogenic differentiation of human adipose stromal/progenitor cells
title_full_unstemmed Fibroblast feeder layer supports adipogenic differentiation of human adipose stromal/progenitor cells
title_short Fibroblast feeder layer supports adipogenic differentiation of human adipose stromal/progenitor cells
title_sort fibroblast feeder layer supports adipogenic differentiation of human adipose stromal/progenitor cells
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768258/
https://www.ncbi.nlm.nih.gov/pubmed/31033380
http://dx.doi.org/10.1080/21623945.2019.1608751
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