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In vitro transdifferentiation of human adipose tissue-derived stem cells to neural lineage cells - a stage-specific incidence

The present Study investigated the intrinsic ability of adipose tissue-derived stem cells (ADSCs) and their neural transdifferentiation in a stage-specific manner. Woodbury’s Chemical induction was implemented with modifications to achieve neural transdifferentiation. In Group I, ADSCs were preinduc...

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Autores principales: Radhakrishnan, Subathra, Trentz, Omana Anna, Reddy, Mettu Srinivas, Rela, Mohamed, Kandasamy, Mahesh, Sellathamby, Shanmugaapriya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768268/
https://www.ncbi.nlm.nih.gov/pubmed/31033391
http://dx.doi.org/10.1080/21623945.2019.1607424
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author Radhakrishnan, Subathra
Trentz, Omana Anna
Reddy, Mettu Srinivas
Rela, Mohamed
Kandasamy, Mahesh
Sellathamby, Shanmugaapriya
author_facet Radhakrishnan, Subathra
Trentz, Omana Anna
Reddy, Mettu Srinivas
Rela, Mohamed
Kandasamy, Mahesh
Sellathamby, Shanmugaapriya
author_sort Radhakrishnan, Subathra
collection PubMed
description The present Study investigated the intrinsic ability of adipose tissue-derived stem cells (ADSCs) and their neural transdifferentiation in a stage-specific manner. Woodbury’s Chemical induction was implemented with modifications to achieve neural transdifferentiation. In Group I, ADSCs were preinduced with β-mercaptoethanol (β-ME) and later, with neural induction medium (NIM). In Group II, ADSCs were directly treated with NIM. In Group III, a DNA methyltransferase (DNMT) inhibitor 5-azacytidine was applied to understand whether transdifferentiation is controlled by epigenetic marks. Irrespective of the presence of (β-ME), the differentiation protocol resulted in glial-lineage cells. Group III produced poorly -differentiated neural cells with neuron-specific enolase positivity. A neuroprogenitor stage (NPC) was identified at d 11 after induction only in Group I. In other groups, this stage was not morphologically distinct. We explored the stage-specific incidence NPC, by alternatively treating them with basic fibroblast growth factor (bFGF), and antioxidants to validate if different signalling could cause varied outcomes (Group IV). They differentiated into neurons, as defined by cell polarity and expression of specific proteins. Meanwhile, neuroprogenitors exposed to NIM (Group I) produced glial-lineage cells. Further refinement and study of the occurrence and terminal differentiation of neuroprogenitors would identify a promising source for neural tissue replacement.
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spelling pubmed-67682682019-10-09 In vitro transdifferentiation of human adipose tissue-derived stem cells to neural lineage cells - a stage-specific incidence Radhakrishnan, Subathra Trentz, Omana Anna Reddy, Mettu Srinivas Rela, Mohamed Kandasamy, Mahesh Sellathamby, Shanmugaapriya Adipocyte Research Paper The present Study investigated the intrinsic ability of adipose tissue-derived stem cells (ADSCs) and their neural transdifferentiation in a stage-specific manner. Woodbury’s Chemical induction was implemented with modifications to achieve neural transdifferentiation. In Group I, ADSCs were preinduced with β-mercaptoethanol (β-ME) and later, with neural induction medium (NIM). In Group II, ADSCs were directly treated with NIM. In Group III, a DNA methyltransferase (DNMT) inhibitor 5-azacytidine was applied to understand whether transdifferentiation is controlled by epigenetic marks. Irrespective of the presence of (β-ME), the differentiation protocol resulted in glial-lineage cells. Group III produced poorly -differentiated neural cells with neuron-specific enolase positivity. A neuroprogenitor stage (NPC) was identified at d 11 after induction only in Group I. In other groups, this stage was not morphologically distinct. We explored the stage-specific incidence NPC, by alternatively treating them with basic fibroblast growth factor (bFGF), and antioxidants to validate if different signalling could cause varied outcomes (Group IV). They differentiated into neurons, as defined by cell polarity and expression of specific proteins. Meanwhile, neuroprogenitors exposed to NIM (Group I) produced glial-lineage cells. Further refinement and study of the occurrence and terminal differentiation of neuroprogenitors would identify a promising source for neural tissue replacement. Taylor & Francis 2019-04-29 /pmc/articles/PMC6768268/ /pubmed/31033391 http://dx.doi.org/10.1080/21623945.2019.1607424 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Radhakrishnan, Subathra
Trentz, Omana Anna
Reddy, Mettu Srinivas
Rela, Mohamed
Kandasamy, Mahesh
Sellathamby, Shanmugaapriya
In vitro transdifferentiation of human adipose tissue-derived stem cells to neural lineage cells - a stage-specific incidence
title In vitro transdifferentiation of human adipose tissue-derived stem cells to neural lineage cells - a stage-specific incidence
title_full In vitro transdifferentiation of human adipose tissue-derived stem cells to neural lineage cells - a stage-specific incidence
title_fullStr In vitro transdifferentiation of human adipose tissue-derived stem cells to neural lineage cells - a stage-specific incidence
title_full_unstemmed In vitro transdifferentiation of human adipose tissue-derived stem cells to neural lineage cells - a stage-specific incidence
title_short In vitro transdifferentiation of human adipose tissue-derived stem cells to neural lineage cells - a stage-specific incidence
title_sort in vitro transdifferentiation of human adipose tissue-derived stem cells to neural lineage cells - a stage-specific incidence
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768268/
https://www.ncbi.nlm.nih.gov/pubmed/31033391
http://dx.doi.org/10.1080/21623945.2019.1607424
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