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MiR-532-3p suppresses colorectal cancer progression by disrupting the ETS1/TGM2 axis-mediated Wnt/β-catenin signaling
The expression panel of plasma microRNA defined miR-532-3p as a valuable biomarker for colorectal adenoma (CRA). However, its expression pattern and function in colorectal cancer (CRC) have remained unclear. The present study investigated the expression levels of miR-532-3p and found that it was in...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768886/ https://www.ncbi.nlm.nih.gov/pubmed/31570702 http://dx.doi.org/10.1038/s41419-019-1962-x |
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author | Gu, Chuncai Cai, Jianqun Xu, Zhijun Zhou, Shiming Ye, Liangying Yan, Qun Zhang, Yue Fang, Yuxin Liu, Yongfeng Tu, Chenge Wang, Xinke He, Juan Li, Qingyuan Han, Lu Lin, Xin Li, Aimin Liu, Side |
author_facet | Gu, Chuncai Cai, Jianqun Xu, Zhijun Zhou, Shiming Ye, Liangying Yan, Qun Zhang, Yue Fang, Yuxin Liu, Yongfeng Tu, Chenge Wang, Xinke He, Juan Li, Qingyuan Han, Lu Lin, Xin Li, Aimin Liu, Side |
author_sort | Gu, Chuncai |
collection | PubMed |
description | The expression panel of plasma microRNA defined miR-532-3p as a valuable biomarker for colorectal adenoma (CRA). However, its expression pattern and function in colorectal cancer (CRC) have remained unclear. The present study investigated the expression levels of miR-532-3p and found that it was in situ downregulated both in CRA and CRC. Moreover, it functioned as a sensitizer for chemotherapy in CRC by inducing cell cycle arrest and early apoptosis via its activating effects on p53 and apoptotic signaling pathways. In addition, miR-532-3p was found to restrain cell growth, metastasis, and epithelial–mesenchymal transition (EMT) phenotype of CRC. A study on the mechanism behind these effects revealed that miR-532-3p directly binds to 3′UTR regions of ETS1 and TGM2, ultimately repressing the canonical Wnt/β-catenin signaling. Further investigation showed that TGM2 was transcriptionally regulated by ETS1 and ETS1/TGM2 axis served as a vital functional target of miR-532-3p in suppressing CRC progression. To conclude, miR-532-3p mimics could act as potential candidate for molecular therapy in CRC through inactivation of the canonical Wnt/β-catenin signaling and enhancement of chemosensitivity. |
format | Online Article Text |
id | pubmed-6768886 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67688862019-10-01 MiR-532-3p suppresses colorectal cancer progression by disrupting the ETS1/TGM2 axis-mediated Wnt/β-catenin signaling Gu, Chuncai Cai, Jianqun Xu, Zhijun Zhou, Shiming Ye, Liangying Yan, Qun Zhang, Yue Fang, Yuxin Liu, Yongfeng Tu, Chenge Wang, Xinke He, Juan Li, Qingyuan Han, Lu Lin, Xin Li, Aimin Liu, Side Cell Death Dis Article The expression panel of plasma microRNA defined miR-532-3p as a valuable biomarker for colorectal adenoma (CRA). However, its expression pattern and function in colorectal cancer (CRC) have remained unclear. The present study investigated the expression levels of miR-532-3p and found that it was in situ downregulated both in CRA and CRC. Moreover, it functioned as a sensitizer for chemotherapy in CRC by inducing cell cycle arrest and early apoptosis via its activating effects on p53 and apoptotic signaling pathways. In addition, miR-532-3p was found to restrain cell growth, metastasis, and epithelial–mesenchymal transition (EMT) phenotype of CRC. A study on the mechanism behind these effects revealed that miR-532-3p directly binds to 3′UTR regions of ETS1 and TGM2, ultimately repressing the canonical Wnt/β-catenin signaling. Further investigation showed that TGM2 was transcriptionally regulated by ETS1 and ETS1/TGM2 axis served as a vital functional target of miR-532-3p in suppressing CRC progression. To conclude, miR-532-3p mimics could act as potential candidate for molecular therapy in CRC through inactivation of the canonical Wnt/β-catenin signaling and enhancement of chemosensitivity. Nature Publishing Group UK 2019-09-30 /pmc/articles/PMC6768886/ /pubmed/31570702 http://dx.doi.org/10.1038/s41419-019-1962-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Gu, Chuncai Cai, Jianqun Xu, Zhijun Zhou, Shiming Ye, Liangying Yan, Qun Zhang, Yue Fang, Yuxin Liu, Yongfeng Tu, Chenge Wang, Xinke He, Juan Li, Qingyuan Han, Lu Lin, Xin Li, Aimin Liu, Side MiR-532-3p suppresses colorectal cancer progression by disrupting the ETS1/TGM2 axis-mediated Wnt/β-catenin signaling |
title | MiR-532-3p suppresses colorectal cancer progression by disrupting the ETS1/TGM2 axis-mediated Wnt/β-catenin signaling |
title_full | MiR-532-3p suppresses colorectal cancer progression by disrupting the ETS1/TGM2 axis-mediated Wnt/β-catenin signaling |
title_fullStr | MiR-532-3p suppresses colorectal cancer progression by disrupting the ETS1/TGM2 axis-mediated Wnt/β-catenin signaling |
title_full_unstemmed | MiR-532-3p suppresses colorectal cancer progression by disrupting the ETS1/TGM2 axis-mediated Wnt/β-catenin signaling |
title_short | MiR-532-3p suppresses colorectal cancer progression by disrupting the ETS1/TGM2 axis-mediated Wnt/β-catenin signaling |
title_sort | mir-532-3p suppresses colorectal cancer progression by disrupting the ets1/tgm2 axis-mediated wnt/β-catenin signaling |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768886/ https://www.ncbi.nlm.nih.gov/pubmed/31570702 http://dx.doi.org/10.1038/s41419-019-1962-x |
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