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Simple lysis of bacterial cells for DNA-based diagnostics using hydrophilic ionic liquids
The extraction of nucleic acids from microorganisms for subsequent molecular diagnostic applications is still a tedious and time-consuming procedure. We developed a method for the rapid preparation of genomic DNA from bacteria based on hydrophilic ionic liquids (ILs). First, we tested eight ILs in d...
Autores principales: | Martzy, Roland, Bica-Schröder, Katharina, Pálvölgyi, Ádám Márk, Kolm, Claudia, Jakwerth, Stefan, Kirschner, Alexander K. T., Sommer, Regina, Krska, Rudolf, Mach, Robert L., Farnleitner, Andreas H., Reischer, Georg H. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768989/ https://www.ncbi.nlm.nih.gov/pubmed/31570727 http://dx.doi.org/10.1038/s41598-019-50246-5 |
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