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Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development

Global microarray gene expression analyses previously demonstrated differences in female and male embryos during neurodevelopment. In particular, before sexual maturation of the gonads, the differences seem to concentrate on the expression of genes encoded on the X- and Y-chromosomes. To investigate...

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Autores principales: Johansson, Martin M., Pottmeier, Philipp, Suciu, Pascalina, Ahmad, Tauseef, Zaghlool, Ammar, Halvardson, Jonatan, Darj, Elisabeth, Feuk, Lars, Peuckert, Christiane, Jazin, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769107/
https://www.ncbi.nlm.nih.gov/pubmed/31608120
http://dx.doi.org/10.3389/fgene.2019.00891
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author Johansson, Martin M.
Pottmeier, Philipp
Suciu, Pascalina
Ahmad, Tauseef
Zaghlool, Ammar
Halvardson, Jonatan
Darj, Elisabeth
Feuk, Lars
Peuckert, Christiane
Jazin, Elena
author_facet Johansson, Martin M.
Pottmeier, Philipp
Suciu, Pascalina
Ahmad, Tauseef
Zaghlool, Ammar
Halvardson, Jonatan
Darj, Elisabeth
Feuk, Lars
Peuckert, Christiane
Jazin, Elena
author_sort Johansson, Martin M.
collection PubMed
description Global microarray gene expression analyses previously demonstrated differences in female and male embryos during neurodevelopment. In particular, before sexual maturation of the gonads, the differences seem to concentrate on the expression of genes encoded on the X- and Y-chromosomes. To investigate genome-wide differences in expression during this early developmental window, we combined high-resolution RNA sequencing with qPCR to analyze brain samples from human embryos during the first trimester of development. Our analysis was tailored for maximum sensitivity to discover Y-chromosome gene expression, but at the same time, it was underpowered to detect X-inactivation escapees. Using this approach, we found that 5 out of 13 expressed gametolog pairs showed unbalanced gene dosage, and as a consequence, a male-biased expression. In addition, we found six novel non-annotated long non-coding RNAs on the Y-chromosome with conserved expression patterns in newborn chimpanzee. The tissue specific and time-restricted expression of these long non-coding RNAs strongly suggests important functions during central nervous system development in human males.
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spelling pubmed-67691072019-10-11 Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development Johansson, Martin M. Pottmeier, Philipp Suciu, Pascalina Ahmad, Tauseef Zaghlool, Ammar Halvardson, Jonatan Darj, Elisabeth Feuk, Lars Peuckert, Christiane Jazin, Elena Front Genet Genetics Global microarray gene expression analyses previously demonstrated differences in female and male embryos during neurodevelopment. In particular, before sexual maturation of the gonads, the differences seem to concentrate on the expression of genes encoded on the X- and Y-chromosomes. To investigate genome-wide differences in expression during this early developmental window, we combined high-resolution RNA sequencing with qPCR to analyze brain samples from human embryos during the first trimester of development. Our analysis was tailored for maximum sensitivity to discover Y-chromosome gene expression, but at the same time, it was underpowered to detect X-inactivation escapees. Using this approach, we found that 5 out of 13 expressed gametolog pairs showed unbalanced gene dosage, and as a consequence, a male-biased expression. In addition, we found six novel non-annotated long non-coding RNAs on the Y-chromosome with conserved expression patterns in newborn chimpanzee. The tissue specific and time-restricted expression of these long non-coding RNAs strongly suggests important functions during central nervous system development in human males. Frontiers Media S.A. 2019-09-24 /pmc/articles/PMC6769107/ /pubmed/31608120 http://dx.doi.org/10.3389/fgene.2019.00891 Text en Copyright © 2019 Johansson, Pottmeier, Suciu, Ahmad, Zaghlool, Halvardson, Darj, Feuk, Peuckert and Jazin http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Johansson, Martin M.
Pottmeier, Philipp
Suciu, Pascalina
Ahmad, Tauseef
Zaghlool, Ammar
Halvardson, Jonatan
Darj, Elisabeth
Feuk, Lars
Peuckert, Christiane
Jazin, Elena
Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development
title Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development
title_full Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development
title_fullStr Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development
title_full_unstemmed Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development
title_short Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development
title_sort novel y-chromosome long non-coding rnas expressed in human male cns during early development
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769107/
https://www.ncbi.nlm.nih.gov/pubmed/31608120
http://dx.doi.org/10.3389/fgene.2019.00891
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