Cargando…
Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development
Global microarray gene expression analyses previously demonstrated differences in female and male embryos during neurodevelopment. In particular, before sexual maturation of the gonads, the differences seem to concentrate on the expression of genes encoded on the X- and Y-chromosomes. To investigate...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769107/ https://www.ncbi.nlm.nih.gov/pubmed/31608120 http://dx.doi.org/10.3389/fgene.2019.00891 |
_version_ | 1783455184709484544 |
---|---|
author | Johansson, Martin M. Pottmeier, Philipp Suciu, Pascalina Ahmad, Tauseef Zaghlool, Ammar Halvardson, Jonatan Darj, Elisabeth Feuk, Lars Peuckert, Christiane Jazin, Elena |
author_facet | Johansson, Martin M. Pottmeier, Philipp Suciu, Pascalina Ahmad, Tauseef Zaghlool, Ammar Halvardson, Jonatan Darj, Elisabeth Feuk, Lars Peuckert, Christiane Jazin, Elena |
author_sort | Johansson, Martin M. |
collection | PubMed |
description | Global microarray gene expression analyses previously demonstrated differences in female and male embryos during neurodevelopment. In particular, before sexual maturation of the gonads, the differences seem to concentrate on the expression of genes encoded on the X- and Y-chromosomes. To investigate genome-wide differences in expression during this early developmental window, we combined high-resolution RNA sequencing with qPCR to analyze brain samples from human embryos during the first trimester of development. Our analysis was tailored for maximum sensitivity to discover Y-chromosome gene expression, but at the same time, it was underpowered to detect X-inactivation escapees. Using this approach, we found that 5 out of 13 expressed gametolog pairs showed unbalanced gene dosage, and as a consequence, a male-biased expression. In addition, we found six novel non-annotated long non-coding RNAs on the Y-chromosome with conserved expression patterns in newborn chimpanzee. The tissue specific and time-restricted expression of these long non-coding RNAs strongly suggests important functions during central nervous system development in human males. |
format | Online Article Text |
id | pubmed-6769107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67691072019-10-11 Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development Johansson, Martin M. Pottmeier, Philipp Suciu, Pascalina Ahmad, Tauseef Zaghlool, Ammar Halvardson, Jonatan Darj, Elisabeth Feuk, Lars Peuckert, Christiane Jazin, Elena Front Genet Genetics Global microarray gene expression analyses previously demonstrated differences in female and male embryos during neurodevelopment. In particular, before sexual maturation of the gonads, the differences seem to concentrate on the expression of genes encoded on the X- and Y-chromosomes. To investigate genome-wide differences in expression during this early developmental window, we combined high-resolution RNA sequencing with qPCR to analyze brain samples from human embryos during the first trimester of development. Our analysis was tailored for maximum sensitivity to discover Y-chromosome gene expression, but at the same time, it was underpowered to detect X-inactivation escapees. Using this approach, we found that 5 out of 13 expressed gametolog pairs showed unbalanced gene dosage, and as a consequence, a male-biased expression. In addition, we found six novel non-annotated long non-coding RNAs on the Y-chromosome with conserved expression patterns in newborn chimpanzee. The tissue specific and time-restricted expression of these long non-coding RNAs strongly suggests important functions during central nervous system development in human males. Frontiers Media S.A. 2019-09-24 /pmc/articles/PMC6769107/ /pubmed/31608120 http://dx.doi.org/10.3389/fgene.2019.00891 Text en Copyright © 2019 Johansson, Pottmeier, Suciu, Ahmad, Zaghlool, Halvardson, Darj, Feuk, Peuckert and Jazin http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Johansson, Martin M. Pottmeier, Philipp Suciu, Pascalina Ahmad, Tauseef Zaghlool, Ammar Halvardson, Jonatan Darj, Elisabeth Feuk, Lars Peuckert, Christiane Jazin, Elena Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development |
title | Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development |
title_full | Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development |
title_fullStr | Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development |
title_full_unstemmed | Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development |
title_short | Novel Y-Chromosome Long Non-Coding RNAs Expressed in Human Male CNS During Early Development |
title_sort | novel y-chromosome long non-coding rnas expressed in human male cns during early development |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769107/ https://www.ncbi.nlm.nih.gov/pubmed/31608120 http://dx.doi.org/10.3389/fgene.2019.00891 |
work_keys_str_mv | AT johanssonmartinm novelychromosomelongnoncodingrnasexpressedinhumanmalecnsduringearlydevelopment AT pottmeierphilipp novelychromosomelongnoncodingrnasexpressedinhumanmalecnsduringearlydevelopment AT suciupascalina novelychromosomelongnoncodingrnasexpressedinhumanmalecnsduringearlydevelopment AT ahmadtauseef novelychromosomelongnoncodingrnasexpressedinhumanmalecnsduringearlydevelopment AT zaghloolammar novelychromosomelongnoncodingrnasexpressedinhumanmalecnsduringearlydevelopment AT halvardsonjonatan novelychromosomelongnoncodingrnasexpressedinhumanmalecnsduringearlydevelopment AT darjelisabeth novelychromosomelongnoncodingrnasexpressedinhumanmalecnsduringearlydevelopment AT feuklars novelychromosomelongnoncodingrnasexpressedinhumanmalecnsduringearlydevelopment AT peuckertchristiane novelychromosomelongnoncodingrnasexpressedinhumanmalecnsduringearlydevelopment AT jazinelena novelychromosomelongnoncodingrnasexpressedinhumanmalecnsduringearlydevelopment |