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Global transcriptional regulation of STAT3- and MYC-mediated sepsis-induced ARDS

BACKGROUND: In recent years, sepsis-induced acute respiratory distress syndrome (ARDS) has remained a major clinical challenge for patients in intensive care units. While some progress has been reported over the years, the pathogenesis of ARDS still needs to be further expounded. METHODS: In the pre...

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Autores principales: Zhang, Jianfeng, Luo, Yifeng, Wang, Xiaoling, Zhu, Jieyun, Li, Qian, Feng, Jihua, He, Dan, Zhong, Zhimei, Zheng, Xiaowen, Lu, Junyu, Zou, Donghua, Luo, Jiefeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769203/
https://www.ncbi.nlm.nih.gov/pubmed/31566109
http://dx.doi.org/10.1177/1753466619879840
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author Zhang, Jianfeng
Luo, Yifeng
Wang, Xiaoling
Zhu, Jieyun
Li, Qian
Feng, Jihua
He, Dan
Zhong, Zhimei
Zheng, Xiaowen
Lu, Junyu
Zou, Donghua
Luo, Jiefeng
author_facet Zhang, Jianfeng
Luo, Yifeng
Wang, Xiaoling
Zhu, Jieyun
Li, Qian
Feng, Jihua
He, Dan
Zhong, Zhimei
Zheng, Xiaowen
Lu, Junyu
Zou, Donghua
Luo, Jiefeng
author_sort Zhang, Jianfeng
collection PubMed
description BACKGROUND: In recent years, sepsis-induced acute respiratory distress syndrome (ARDS) has remained a major clinical challenge for patients in intensive care units. While some progress has been reported over the years, the pathogenesis of ARDS still needs to be further expounded. METHODS: In the present study, gene set enrichment analysis, differentially expressed genes analysis, short time-series expression miner, protein–protein interaction (PPI) networks, module analysis, hypergeometric test, and functional enrichment analysis were performed in whole blood gene expression profiles of sepsis and induced-sepsis ARDS to explore the molecular mechanism of sepsis-induced ARDS. RESULTS: Further dysregulated genes in the process evolving from healthy control through sepsis to sepsis-induced ARDS were identified and organized into 10 functional modules based on their PPI networks. These functional modules were significantly involved in cell cycle, ubiquitin mediated proteolysis, spliceosome, and other pathways. MYC, STAT3, LEF1, and BRCA1 were potential transcription factors (TFs) regulating these modules. A TF-module-pathway global regulation network was constructed. In particular, our findings suggest that MYC and STAT3 may be the key regulatory genes in the underlying dysfunction of sepsis-induced ARDS. Receiver operating characteristic curve analysis showed the core genes in the global regulation network may be biomarkers for sepsis or sepsis-induced ARDS. CONCLUSIONS: We found that MYC and STAT3 may be the key regulatory genes in the underlying dysfunction of sepsis-induced ARDS. The reviews of this paper are available via the supplementary material section.
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spelling pubmed-67692032019-10-18 Global transcriptional regulation of STAT3- and MYC-mediated sepsis-induced ARDS Zhang, Jianfeng Luo, Yifeng Wang, Xiaoling Zhu, Jieyun Li, Qian Feng, Jihua He, Dan Zhong, Zhimei Zheng, Xiaowen Lu, Junyu Zou, Donghua Luo, Jiefeng Ther Adv Respir Dis Original Research BACKGROUND: In recent years, sepsis-induced acute respiratory distress syndrome (ARDS) has remained a major clinical challenge for patients in intensive care units. While some progress has been reported over the years, the pathogenesis of ARDS still needs to be further expounded. METHODS: In the present study, gene set enrichment analysis, differentially expressed genes analysis, short time-series expression miner, protein–protein interaction (PPI) networks, module analysis, hypergeometric test, and functional enrichment analysis were performed in whole blood gene expression profiles of sepsis and induced-sepsis ARDS to explore the molecular mechanism of sepsis-induced ARDS. RESULTS: Further dysregulated genes in the process evolving from healthy control through sepsis to sepsis-induced ARDS were identified and organized into 10 functional modules based on their PPI networks. These functional modules were significantly involved in cell cycle, ubiquitin mediated proteolysis, spliceosome, and other pathways. MYC, STAT3, LEF1, and BRCA1 were potential transcription factors (TFs) regulating these modules. A TF-module-pathway global regulation network was constructed. In particular, our findings suggest that MYC and STAT3 may be the key regulatory genes in the underlying dysfunction of sepsis-induced ARDS. Receiver operating characteristic curve analysis showed the core genes in the global regulation network may be biomarkers for sepsis or sepsis-induced ARDS. CONCLUSIONS: We found that MYC and STAT3 may be the key regulatory genes in the underlying dysfunction of sepsis-induced ARDS. The reviews of this paper are available via the supplementary material section. SAGE Publications 2019-09-30 /pmc/articles/PMC6769203/ /pubmed/31566109 http://dx.doi.org/10.1177/1753466619879840 Text en © The Author(s), 2019 http://www.creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research
Zhang, Jianfeng
Luo, Yifeng
Wang, Xiaoling
Zhu, Jieyun
Li, Qian
Feng, Jihua
He, Dan
Zhong, Zhimei
Zheng, Xiaowen
Lu, Junyu
Zou, Donghua
Luo, Jiefeng
Global transcriptional regulation of STAT3- and MYC-mediated sepsis-induced ARDS
title Global transcriptional regulation of STAT3- and MYC-mediated sepsis-induced ARDS
title_full Global transcriptional regulation of STAT3- and MYC-mediated sepsis-induced ARDS
title_fullStr Global transcriptional regulation of STAT3- and MYC-mediated sepsis-induced ARDS
title_full_unstemmed Global transcriptional regulation of STAT3- and MYC-mediated sepsis-induced ARDS
title_short Global transcriptional regulation of STAT3- and MYC-mediated sepsis-induced ARDS
title_sort global transcriptional regulation of stat3- and myc-mediated sepsis-induced ards
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769203/
https://www.ncbi.nlm.nih.gov/pubmed/31566109
http://dx.doi.org/10.1177/1753466619879840
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