Cargando…

Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of Cytokine Signaling 3 and the Long Form of the Leptin Receptor

We hypothesized that resistin is engaged in the development of leptin central insensitivity/resistance in sheep, which is a unique animal model to explore reversible leptin resistance. Thirty Polish Longwool ewes, which were ovariectomized with estrogen replacement, were used. Treatments consisted o...

Descripción completa

Detalles Bibliográficos
Autores principales: Zieba, Dorota Anna, Biernat, Weronika, Szczesna, Malgorzata, Kirsz, Katarzyna, Misztal, Tomasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769434/
https://www.ncbi.nlm.nih.gov/pubmed/31514318
http://dx.doi.org/10.3390/nu11092180
_version_ 1783455236115922944
author Zieba, Dorota Anna
Biernat, Weronika
Szczesna, Malgorzata
Kirsz, Katarzyna
Misztal, Tomasz
author_facet Zieba, Dorota Anna
Biernat, Weronika
Szczesna, Malgorzata
Kirsz, Katarzyna
Misztal, Tomasz
author_sort Zieba, Dorota Anna
collection PubMed
description We hypothesized that resistin is engaged in the development of leptin central insensitivity/resistance in sheep, which is a unique animal model to explore reversible leptin resistance. Thirty Polish Longwool ewes, which were ovariectomized with estrogen replacement, were used. Treatments consisted of the intravenous injection of control (saline) or recombinant bovine resistin (rbresistin): control (Control; n = 10), a low dose of rbresistin (R1; 1.0 μg/kg body weight (BW); n = 10), and a high dose of rbresistin (R2; 10.0 μg/kg BW; n = 10). The studies were performed during short-day (SD) and long-day (LD) photoperiods. Leptin and resistin concentrations were determined. Expression levels of a suppressor of cytokine signaling (SOCS)-3 and the long form of the leptin receptor (LeptRb) were determined in selected brain regions, including in the anterior pituitary (AP), hypothalamic arcuate nucleus (ARC), preoptic area (POA), and ventro- and dorsomedial nuclei (VMH/DMH). The results indicate that resistin induced a consistent decrease in LeptRb (except in POA) and an increase in SOCS-3 expression during the LD photoperiod in all selected brain regions. In conclusion, the results demonstrate that the action of resistin appears to be strongly associated with photoperiod-driven changes in the leptin signaling pathway, which may underlie the phenomenon of central leptin resistance.
format Online
Article
Text
id pubmed-6769434
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-67694342019-10-30 Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of Cytokine Signaling 3 and the Long Form of the Leptin Receptor Zieba, Dorota Anna Biernat, Weronika Szczesna, Malgorzata Kirsz, Katarzyna Misztal, Tomasz Nutrients Article We hypothesized that resistin is engaged in the development of leptin central insensitivity/resistance in sheep, which is a unique animal model to explore reversible leptin resistance. Thirty Polish Longwool ewes, which were ovariectomized with estrogen replacement, were used. Treatments consisted of the intravenous injection of control (saline) or recombinant bovine resistin (rbresistin): control (Control; n = 10), a low dose of rbresistin (R1; 1.0 μg/kg body weight (BW); n = 10), and a high dose of rbresistin (R2; 10.0 μg/kg BW; n = 10). The studies were performed during short-day (SD) and long-day (LD) photoperiods. Leptin and resistin concentrations were determined. Expression levels of a suppressor of cytokine signaling (SOCS)-3 and the long form of the leptin receptor (LeptRb) were determined in selected brain regions, including in the anterior pituitary (AP), hypothalamic arcuate nucleus (ARC), preoptic area (POA), and ventro- and dorsomedial nuclei (VMH/DMH). The results indicate that resistin induced a consistent decrease in LeptRb (except in POA) and an increase in SOCS-3 expression during the LD photoperiod in all selected brain regions. In conclusion, the results demonstrate that the action of resistin appears to be strongly associated with photoperiod-driven changes in the leptin signaling pathway, which may underlie the phenomenon of central leptin resistance. MDPI 2019-09-11 /pmc/articles/PMC6769434/ /pubmed/31514318 http://dx.doi.org/10.3390/nu11092180 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zieba, Dorota Anna
Biernat, Weronika
Szczesna, Malgorzata
Kirsz, Katarzyna
Misztal, Tomasz
Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of Cytokine Signaling 3 and the Long Form of the Leptin Receptor
title Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of Cytokine Signaling 3 and the Long Form of the Leptin Receptor
title_full Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of Cytokine Signaling 3 and the Long Form of the Leptin Receptor
title_fullStr Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of Cytokine Signaling 3 and the Long Form of the Leptin Receptor
title_full_unstemmed Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of Cytokine Signaling 3 and the Long Form of the Leptin Receptor
title_short Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of Cytokine Signaling 3 and the Long Form of the Leptin Receptor
title_sort hypothalamic–pituitary and adipose tissue responses to the effect of resistin in sheep: the integration of leptin and resistin signaling involving a suppressor of cytokine signaling 3 and the long form of the leptin receptor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769434/
https://www.ncbi.nlm.nih.gov/pubmed/31514318
http://dx.doi.org/10.3390/nu11092180
work_keys_str_mv AT ziebadorotaanna hypothalamicpituitaryandadiposetissueresponsestotheeffectofresistininsheeptheintegrationofleptinandresistinsignalinginvolvingasuppressorofcytokinesignaling3andthelongformoftheleptinreceptor
AT biernatweronika hypothalamicpituitaryandadiposetissueresponsestotheeffectofresistininsheeptheintegrationofleptinandresistinsignalinginvolvingasuppressorofcytokinesignaling3andthelongformoftheleptinreceptor
AT szczesnamalgorzata hypothalamicpituitaryandadiposetissueresponsestotheeffectofresistininsheeptheintegrationofleptinandresistinsignalinginvolvingasuppressorofcytokinesignaling3andthelongformoftheleptinreceptor
AT kirszkatarzyna hypothalamicpituitaryandadiposetissueresponsestotheeffectofresistininsheeptheintegrationofleptinandresistinsignalinginvolvingasuppressorofcytokinesignaling3andthelongformoftheleptinreceptor
AT misztaltomasz hypothalamicpituitaryandadiposetissueresponsestotheeffectofresistininsheeptheintegrationofleptinandresistinsignalinginvolvingasuppressorofcytokinesignaling3andthelongformoftheleptinreceptor