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Isoflavone Aglycones Attenuate Cigarette Smoke-Induced Emphysema via Suppression of Neutrophilic Inflammation in a COPD Murine Model

Chronic obstructive pulmonary disease (COPD), a lung disease caused by chronic exposure to cigarette smoke, increases the number of inflammatory cells such as macrophages and neutrophils and emphysema. Isoflavone is a polyphenolic compound that exists in soybeans. Daidzein and genistein, two types o...

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Autores principales: Kojima, Kazuya, Asai, Kazuhisa, Kubo, Hiroaki, Sugitani, Arata, Kyomoto, Yohkoh, Okamoto, Atsuko, Yamada, Kazuhiro, Ijiri, Naoki, Watanabe, Tetsuya, Hirata, Kazuto, Kawaguchi, Tomoya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769447/
https://www.ncbi.nlm.nih.gov/pubmed/31470503
http://dx.doi.org/10.3390/nu11092023
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author Kojima, Kazuya
Asai, Kazuhisa
Kubo, Hiroaki
Sugitani, Arata
Kyomoto, Yohkoh
Okamoto, Atsuko
Yamada, Kazuhiro
Ijiri, Naoki
Watanabe, Tetsuya
Hirata, Kazuto
Kawaguchi, Tomoya
author_facet Kojima, Kazuya
Asai, Kazuhisa
Kubo, Hiroaki
Sugitani, Arata
Kyomoto, Yohkoh
Okamoto, Atsuko
Yamada, Kazuhiro
Ijiri, Naoki
Watanabe, Tetsuya
Hirata, Kazuto
Kawaguchi, Tomoya
author_sort Kojima, Kazuya
collection PubMed
description Chronic obstructive pulmonary disease (COPD), a lung disease caused by chronic exposure to cigarette smoke, increases the number of inflammatory cells such as macrophages and neutrophils and emphysema. Isoflavone is a polyphenolic compound that exists in soybeans. Daidzein and genistein, two types of isoflavones, have been reported to have anti-inflammatory effects in various organs. We hypothesized that the daidzein-rich soy isoflavone aglycones (DRIAs) attenuate cigarette smoke-induced emphysema in mice. Mice were divided into four groups: the (i) control group, (ii) isoflavone group, (iii) smoking group, and (iv) isoflavone + smoking group. The number of inflammatory cells in the bronchoalveolar lavage fluid (BALF) and the airspace enlargement using the mean linear intercept (MLI) were determined 12 weeks after smoking exposure. Expressions of neutrophilic inflammatory cytokines and chemokines were also examined. In the isoflavone + smoking group, the number of neutrophils in BALF and MLI was significantly less than that in the smoking group. Furthermore, the gene-expressions of TNF-α and CXCL2 (MIP-2) in the isoflavone + smoking group were significantly less than those in the smoking group. Supplementation of the COPD murine model with DRIAs significantly attenuates pathological changes of COPD via suppression of neutrophilic inflammation.
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spelling pubmed-67694472019-10-30 Isoflavone Aglycones Attenuate Cigarette Smoke-Induced Emphysema via Suppression of Neutrophilic Inflammation in a COPD Murine Model Kojima, Kazuya Asai, Kazuhisa Kubo, Hiroaki Sugitani, Arata Kyomoto, Yohkoh Okamoto, Atsuko Yamada, Kazuhiro Ijiri, Naoki Watanabe, Tetsuya Hirata, Kazuto Kawaguchi, Tomoya Nutrients Article Chronic obstructive pulmonary disease (COPD), a lung disease caused by chronic exposure to cigarette smoke, increases the number of inflammatory cells such as macrophages and neutrophils and emphysema. Isoflavone is a polyphenolic compound that exists in soybeans. Daidzein and genistein, two types of isoflavones, have been reported to have anti-inflammatory effects in various organs. We hypothesized that the daidzein-rich soy isoflavone aglycones (DRIAs) attenuate cigarette smoke-induced emphysema in mice. Mice were divided into four groups: the (i) control group, (ii) isoflavone group, (iii) smoking group, and (iv) isoflavone + smoking group. The number of inflammatory cells in the bronchoalveolar lavage fluid (BALF) and the airspace enlargement using the mean linear intercept (MLI) were determined 12 weeks after smoking exposure. Expressions of neutrophilic inflammatory cytokines and chemokines were also examined. In the isoflavone + smoking group, the number of neutrophils in BALF and MLI was significantly less than that in the smoking group. Furthermore, the gene-expressions of TNF-α and CXCL2 (MIP-2) in the isoflavone + smoking group were significantly less than those in the smoking group. Supplementation of the COPD murine model with DRIAs significantly attenuates pathological changes of COPD via suppression of neutrophilic inflammation. MDPI 2019-08-29 /pmc/articles/PMC6769447/ /pubmed/31470503 http://dx.doi.org/10.3390/nu11092023 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kojima, Kazuya
Asai, Kazuhisa
Kubo, Hiroaki
Sugitani, Arata
Kyomoto, Yohkoh
Okamoto, Atsuko
Yamada, Kazuhiro
Ijiri, Naoki
Watanabe, Tetsuya
Hirata, Kazuto
Kawaguchi, Tomoya
Isoflavone Aglycones Attenuate Cigarette Smoke-Induced Emphysema via Suppression of Neutrophilic Inflammation in a COPD Murine Model
title Isoflavone Aglycones Attenuate Cigarette Smoke-Induced Emphysema via Suppression of Neutrophilic Inflammation in a COPD Murine Model
title_full Isoflavone Aglycones Attenuate Cigarette Smoke-Induced Emphysema via Suppression of Neutrophilic Inflammation in a COPD Murine Model
title_fullStr Isoflavone Aglycones Attenuate Cigarette Smoke-Induced Emphysema via Suppression of Neutrophilic Inflammation in a COPD Murine Model
title_full_unstemmed Isoflavone Aglycones Attenuate Cigarette Smoke-Induced Emphysema via Suppression of Neutrophilic Inflammation in a COPD Murine Model
title_short Isoflavone Aglycones Attenuate Cigarette Smoke-Induced Emphysema via Suppression of Neutrophilic Inflammation in a COPD Murine Model
title_sort isoflavone aglycones attenuate cigarette smoke-induced emphysema via suppression of neutrophilic inflammation in a copd murine model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769447/
https://www.ncbi.nlm.nih.gov/pubmed/31470503
http://dx.doi.org/10.3390/nu11092023
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