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E-cadherin Downregulation and microRNAs in Sporadic Intestinal-Type Gastric Cancer
CDH1 gene, encoding E-cadherin, is a tumor suppressor gene frequently altered in gastric cancers (GCs) of both diffuse (DGC) and intestinal (IGC) histotypes, albeit through different mechanisms. The study aimed to characterize CDH1 expression in sporadic IGC and to investigate whether microRNAs (miR...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769612/ https://www.ncbi.nlm.nih.gov/pubmed/31509966 http://dx.doi.org/10.3390/ijms20184452 |
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author | Rossi, Tania Tedaldi, Gianluca Petracci, Elisabetta Abou Khouzam, Raefa Ranzani, Guglielmina Nadia Morgagni, Paolo Saragoni, Luca Monti, Manlio Calistri, Daniele Ulivi, Paola Molinari, Chiara |
author_facet | Rossi, Tania Tedaldi, Gianluca Petracci, Elisabetta Abou Khouzam, Raefa Ranzani, Guglielmina Nadia Morgagni, Paolo Saragoni, Luca Monti, Manlio Calistri, Daniele Ulivi, Paola Molinari, Chiara |
author_sort | Rossi, Tania |
collection | PubMed |
description | CDH1 gene, encoding E-cadherin, is a tumor suppressor gene frequently altered in gastric cancers (GCs) of both diffuse (DGC) and intestinal (IGC) histotypes, albeit through different mechanisms. The study aimed to characterize CDH1 expression in sporadic IGC and to investigate whether microRNAs (miRs) are involved in its transcriptional control. We evaluated CDH1 expression by quantitative real-time PCR (RT-qPCR) in 33 IGC patients and found a significant downregulation in tumor tissues compared to normal counterparts (p-value = 0.025). Moreover, 14 miRs, predicted to be involved in CDH1 regulation in both a direct and indirect manner, were selected and analyzed by RT-qPCR in an independent case series of 17 IGCs and matched normal tissues. miR-101, miR-26b, and miR-200c emerged as significantly downregulated and were confirmed in the case series of 33 patients (p-value < 0.001). Finally, we evaluated EZH2 expression, a target of both miR-101 and miR-26b, which showed significant upregulation in IGCs (p-value = 0.005). A significant inverse correlation was observed between EZH2 overexpression and CDH1, miR-101, and miR-26b levels (p-value < 0.001). Our results reinforce the link between CDH1 and IGC, highlighting the role of miRs in its transcriptional control and improving our understanding of GC subtypes and biomarkers. |
format | Online Article Text |
id | pubmed-6769612 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67696122019-10-30 E-cadherin Downregulation and microRNAs in Sporadic Intestinal-Type Gastric Cancer Rossi, Tania Tedaldi, Gianluca Petracci, Elisabetta Abou Khouzam, Raefa Ranzani, Guglielmina Nadia Morgagni, Paolo Saragoni, Luca Monti, Manlio Calistri, Daniele Ulivi, Paola Molinari, Chiara Int J Mol Sci Article CDH1 gene, encoding E-cadherin, is a tumor suppressor gene frequently altered in gastric cancers (GCs) of both diffuse (DGC) and intestinal (IGC) histotypes, albeit through different mechanisms. The study aimed to characterize CDH1 expression in sporadic IGC and to investigate whether microRNAs (miRs) are involved in its transcriptional control. We evaluated CDH1 expression by quantitative real-time PCR (RT-qPCR) in 33 IGC patients and found a significant downregulation in tumor tissues compared to normal counterparts (p-value = 0.025). Moreover, 14 miRs, predicted to be involved in CDH1 regulation in both a direct and indirect manner, were selected and analyzed by RT-qPCR in an independent case series of 17 IGCs and matched normal tissues. miR-101, miR-26b, and miR-200c emerged as significantly downregulated and were confirmed in the case series of 33 patients (p-value < 0.001). Finally, we evaluated EZH2 expression, a target of both miR-101 and miR-26b, which showed significant upregulation in IGCs (p-value = 0.005). A significant inverse correlation was observed between EZH2 overexpression and CDH1, miR-101, and miR-26b levels (p-value < 0.001). Our results reinforce the link between CDH1 and IGC, highlighting the role of miRs in its transcriptional control and improving our understanding of GC subtypes and biomarkers. MDPI 2019-09-10 /pmc/articles/PMC6769612/ /pubmed/31509966 http://dx.doi.org/10.3390/ijms20184452 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rossi, Tania Tedaldi, Gianluca Petracci, Elisabetta Abou Khouzam, Raefa Ranzani, Guglielmina Nadia Morgagni, Paolo Saragoni, Luca Monti, Manlio Calistri, Daniele Ulivi, Paola Molinari, Chiara E-cadherin Downregulation and microRNAs in Sporadic Intestinal-Type Gastric Cancer |
title | E-cadherin Downregulation and microRNAs in Sporadic Intestinal-Type Gastric Cancer |
title_full | E-cadherin Downregulation and microRNAs in Sporadic Intestinal-Type Gastric Cancer |
title_fullStr | E-cadherin Downregulation and microRNAs in Sporadic Intestinal-Type Gastric Cancer |
title_full_unstemmed | E-cadherin Downregulation and microRNAs in Sporadic Intestinal-Type Gastric Cancer |
title_short | E-cadherin Downregulation and microRNAs in Sporadic Intestinal-Type Gastric Cancer |
title_sort | e-cadherin downregulation and micrornas in sporadic intestinal-type gastric cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769612/ https://www.ncbi.nlm.nih.gov/pubmed/31509966 http://dx.doi.org/10.3390/ijms20184452 |
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