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Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm

Antibiotic resistance is currently one of the main threats to public health security. Biofilm formation is a resistance mechanism that is responsible for most human bacterial infections and requires new and effective therapeutic approaches, such as those provided by nanotechnology. In this work, the...

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Autores principales: Llamazares, Celia, Sanz del Olmo, Natalia, Ortega, Paula, Gómez, Rafael, Soliveri, Juan, de la Mata, F. Javier, García-Gallego, Sandra, Copa-Patiño, José Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769849/
https://www.ncbi.nlm.nih.gov/pubmed/31450779
http://dx.doi.org/10.3390/biom9090405
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author Llamazares, Celia
Sanz del Olmo, Natalia
Ortega, Paula
Gómez, Rafael
Soliveri, Juan
de la Mata, F. Javier
García-Gallego, Sandra
Copa-Patiño, José Luis
author_facet Llamazares, Celia
Sanz del Olmo, Natalia
Ortega, Paula
Gómez, Rafael
Soliveri, Juan
de la Mata, F. Javier
García-Gallego, Sandra
Copa-Patiño, José Luis
author_sort Llamazares, Celia
collection PubMed
description Antibiotic resistance is currently one of the main threats to public health security. Biofilm formation is a resistance mechanism that is responsible for most human bacterial infections and requires new and effective therapeutic approaches, such as those provided by nanotechnology. In this work, the antibacterial effect of carbosilane metallodendrimers with different metals (copper(II) and ruthenium(II)), ligands (chloride and nitrate) and generations (generation 0, 1 and 2) has been studied using planktonic Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. Furthermore, the ability of the metallodendrimers to avoid the formation of S. aureus biofilms was also evaluated. The results showed a promising biocide activity in both types of planktonic bacteria, especially for first-generation dendrimers, which arises from the metal complexation to the dendrimer. Cu(II) metallodendrimers require lower concentration than Ru(II) counterpart to inhibit the production of S. aureus biofilms, but none produce hemolysis at the inhibitory concentrations and can be safely used as antibacterial agents. In particular, the first-generation Cu(II) metallodendrimer with nitrate ligands displayed the most promising properties to continue with further studies in both planktonic cells and biofilms.
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spelling pubmed-67698492019-10-30 Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm Llamazares, Celia Sanz del Olmo, Natalia Ortega, Paula Gómez, Rafael Soliveri, Juan de la Mata, F. Javier García-Gallego, Sandra Copa-Patiño, José Luis Biomolecules Article Antibiotic resistance is currently one of the main threats to public health security. Biofilm formation is a resistance mechanism that is responsible for most human bacterial infections and requires new and effective therapeutic approaches, such as those provided by nanotechnology. In this work, the antibacterial effect of carbosilane metallodendrimers with different metals (copper(II) and ruthenium(II)), ligands (chloride and nitrate) and generations (generation 0, 1 and 2) has been studied using planktonic Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. Furthermore, the ability of the metallodendrimers to avoid the formation of S. aureus biofilms was also evaluated. The results showed a promising biocide activity in both types of planktonic bacteria, especially for first-generation dendrimers, which arises from the metal complexation to the dendrimer. Cu(II) metallodendrimers require lower concentration than Ru(II) counterpart to inhibit the production of S. aureus biofilms, but none produce hemolysis at the inhibitory concentrations and can be safely used as antibacterial agents. In particular, the first-generation Cu(II) metallodendrimer with nitrate ligands displayed the most promising properties to continue with further studies in both planktonic cells and biofilms. MDPI 2019-08-23 /pmc/articles/PMC6769849/ /pubmed/31450779 http://dx.doi.org/10.3390/biom9090405 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Llamazares, Celia
Sanz del Olmo, Natalia
Ortega, Paula
Gómez, Rafael
Soliveri, Juan
de la Mata, F. Javier
García-Gallego, Sandra
Copa-Patiño, José Luis
Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm
title Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm
title_full Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm
title_fullStr Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm
title_full_unstemmed Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm
title_short Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm
title_sort antibacterial effect of carbosilane metallodendrimers in planktonic cells of gram-positive and gram-negative bacteria and staphylococcus aureus biofilm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769849/
https://www.ncbi.nlm.nih.gov/pubmed/31450779
http://dx.doi.org/10.3390/biom9090405
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