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Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization

The xylose operon is an efficient biological element used for the regulation of gene expression in Bacillus licheniformis. Although the mechanism underlying the xylose-mediated regulation of this operon has been elucidated, the transcriptional changes that occur under various fermentation conditions...

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Autores principales: Li, Youran, Liu, Xiang, Zhang, Liang, Ding, Zhongyang, Xu, Sha, Gu, Zhenghua, Shi, Guiyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769896/
https://www.ncbi.nlm.nih.gov/pubmed/31540366
http://dx.doi.org/10.3390/ijms20184615
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author Li, Youran
Liu, Xiang
Zhang, Liang
Ding, Zhongyang
Xu, Sha
Gu, Zhenghua
Shi, Guiyang
author_facet Li, Youran
Liu, Xiang
Zhang, Liang
Ding, Zhongyang
Xu, Sha
Gu, Zhenghua
Shi, Guiyang
author_sort Li, Youran
collection PubMed
description The xylose operon is an efficient biological element used for the regulation of gene expression in Bacillus licheniformis. Although the mechanism underlying the xylose-mediated regulation of this operon has been elucidated, the transcriptional changes that occur under various fermentation conditions remain unclear. In this study, the effects of different conditions on xylose operon expression were investigated. Significant upregulation was observed during the transition from the logarithmic phase to the stationary phase (2.5-fold, n = 3, p < 0.01). Glucose suppressed transcription over 168-fold (n = 3, p < 0.01). Meanwhile, the inhibitory effect of glucose hardly strengthened at concentrations from 20 to 180 g/L. Furthermore, the transcription of the xylose operon increased at elevated temperatures (25–42 °C) and was optimal at a neutral pH (pH 6.5–7.0). Based on these findings, relevant fermentation strategies (delaying the induction time, using dextrin as a carbon source, increasing the fermentation temperature, and maintaining a neutral pH) were proposed. Subsequently, these strategies were validated through the use of maltogenic amylase as a reporter protein, as an 8-fold (n = 3, p < 0.01) increase in recombinant enzyme activity compared to that under unoptimized conditions was observed. This work contributes to the development of fermentation optimization and furthers the use of the xylose operon as an efficient expression element.
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spelling pubmed-67698962019-10-30 Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization Li, Youran Liu, Xiang Zhang, Liang Ding, Zhongyang Xu, Sha Gu, Zhenghua Shi, Guiyang Int J Mol Sci Article The xylose operon is an efficient biological element used for the regulation of gene expression in Bacillus licheniformis. Although the mechanism underlying the xylose-mediated regulation of this operon has been elucidated, the transcriptional changes that occur under various fermentation conditions remain unclear. In this study, the effects of different conditions on xylose operon expression were investigated. Significant upregulation was observed during the transition from the logarithmic phase to the stationary phase (2.5-fold, n = 3, p < 0.01). Glucose suppressed transcription over 168-fold (n = 3, p < 0.01). Meanwhile, the inhibitory effect of glucose hardly strengthened at concentrations from 20 to 180 g/L. Furthermore, the transcription of the xylose operon increased at elevated temperatures (25–42 °C) and was optimal at a neutral pH (pH 6.5–7.0). Based on these findings, relevant fermentation strategies (delaying the induction time, using dextrin as a carbon source, increasing the fermentation temperature, and maintaining a neutral pH) were proposed. Subsequently, these strategies were validated through the use of maltogenic amylase as a reporter protein, as an 8-fold (n = 3, p < 0.01) increase in recombinant enzyme activity compared to that under unoptimized conditions was observed. This work contributes to the development of fermentation optimization and furthers the use of the xylose operon as an efficient expression element. MDPI 2019-09-18 /pmc/articles/PMC6769896/ /pubmed/31540366 http://dx.doi.org/10.3390/ijms20184615 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Youran
Liu, Xiang
Zhang, Liang
Ding, Zhongyang
Xu, Sha
Gu, Zhenghua
Shi, Guiyang
Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization
title Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization
title_full Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization
title_fullStr Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization
title_full_unstemmed Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization
title_short Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization
title_sort transcriptional changes in the xylose operon in bacillus licheniformis and their use in fermentation optimization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769896/
https://www.ncbi.nlm.nih.gov/pubmed/31540366
http://dx.doi.org/10.3390/ijms20184615
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