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Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization
The xylose operon is an efficient biological element used for the regulation of gene expression in Bacillus licheniformis. Although the mechanism underlying the xylose-mediated regulation of this operon has been elucidated, the transcriptional changes that occur under various fermentation conditions...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769896/ https://www.ncbi.nlm.nih.gov/pubmed/31540366 http://dx.doi.org/10.3390/ijms20184615 |
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author | Li, Youran Liu, Xiang Zhang, Liang Ding, Zhongyang Xu, Sha Gu, Zhenghua Shi, Guiyang |
author_facet | Li, Youran Liu, Xiang Zhang, Liang Ding, Zhongyang Xu, Sha Gu, Zhenghua Shi, Guiyang |
author_sort | Li, Youran |
collection | PubMed |
description | The xylose operon is an efficient biological element used for the regulation of gene expression in Bacillus licheniformis. Although the mechanism underlying the xylose-mediated regulation of this operon has been elucidated, the transcriptional changes that occur under various fermentation conditions remain unclear. In this study, the effects of different conditions on xylose operon expression were investigated. Significant upregulation was observed during the transition from the logarithmic phase to the stationary phase (2.5-fold, n = 3, p < 0.01). Glucose suppressed transcription over 168-fold (n = 3, p < 0.01). Meanwhile, the inhibitory effect of glucose hardly strengthened at concentrations from 20 to 180 g/L. Furthermore, the transcription of the xylose operon increased at elevated temperatures (25–42 °C) and was optimal at a neutral pH (pH 6.5–7.0). Based on these findings, relevant fermentation strategies (delaying the induction time, using dextrin as a carbon source, increasing the fermentation temperature, and maintaining a neutral pH) were proposed. Subsequently, these strategies were validated through the use of maltogenic amylase as a reporter protein, as an 8-fold (n = 3, p < 0.01) increase in recombinant enzyme activity compared to that under unoptimized conditions was observed. This work contributes to the development of fermentation optimization and furthers the use of the xylose operon as an efficient expression element. |
format | Online Article Text |
id | pubmed-6769896 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67698962019-10-30 Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization Li, Youran Liu, Xiang Zhang, Liang Ding, Zhongyang Xu, Sha Gu, Zhenghua Shi, Guiyang Int J Mol Sci Article The xylose operon is an efficient biological element used for the regulation of gene expression in Bacillus licheniformis. Although the mechanism underlying the xylose-mediated regulation of this operon has been elucidated, the transcriptional changes that occur under various fermentation conditions remain unclear. In this study, the effects of different conditions on xylose operon expression were investigated. Significant upregulation was observed during the transition from the logarithmic phase to the stationary phase (2.5-fold, n = 3, p < 0.01). Glucose suppressed transcription over 168-fold (n = 3, p < 0.01). Meanwhile, the inhibitory effect of glucose hardly strengthened at concentrations from 20 to 180 g/L. Furthermore, the transcription of the xylose operon increased at elevated temperatures (25–42 °C) and was optimal at a neutral pH (pH 6.5–7.0). Based on these findings, relevant fermentation strategies (delaying the induction time, using dextrin as a carbon source, increasing the fermentation temperature, and maintaining a neutral pH) were proposed. Subsequently, these strategies were validated through the use of maltogenic amylase as a reporter protein, as an 8-fold (n = 3, p < 0.01) increase in recombinant enzyme activity compared to that under unoptimized conditions was observed. This work contributes to the development of fermentation optimization and furthers the use of the xylose operon as an efficient expression element. MDPI 2019-09-18 /pmc/articles/PMC6769896/ /pubmed/31540366 http://dx.doi.org/10.3390/ijms20184615 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Youran Liu, Xiang Zhang, Liang Ding, Zhongyang Xu, Sha Gu, Zhenghua Shi, Guiyang Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization |
title | Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization |
title_full | Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization |
title_fullStr | Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization |
title_full_unstemmed | Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization |
title_short | Transcriptional Changes in the Xylose Operon in Bacillus licheniformis and Their Use in Fermentation Optimization |
title_sort | transcriptional changes in the xylose operon in bacillus licheniformis and their use in fermentation optimization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769896/ https://www.ncbi.nlm.nih.gov/pubmed/31540366 http://dx.doi.org/10.3390/ijms20184615 |
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