Cargando…
P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells
Resveratrol has been proposed to prevent tumor growth and the different steps of carcinogenesis; nevertheless, these biological effects are sometimes discordant between different cell types. Several hypotheses and works have suggested that the metabolism of resveratrol could be at the origin of a di...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770091/ https://www.ncbi.nlm.nih.gov/pubmed/31487863 http://dx.doi.org/10.3390/nu11092098 |
_version_ | 1783455390214651904 |
---|---|
author | Aires, Virginie Colin, Didier J Doreau, Agnès Di Pietro, Attilio Heydel, Jean-Marie Artur, Yves Latruffe, Norbert Delmas, Dominique |
author_facet | Aires, Virginie Colin, Didier J Doreau, Agnès Di Pietro, Attilio Heydel, Jean-Marie Artur, Yves Latruffe, Norbert Delmas, Dominique |
author_sort | Aires, Virginie |
collection | PubMed |
description | Resveratrol has been proposed to prevent tumor growth and the different steps of carcinogenesis; nevertheless, these biological effects are sometimes discordant between different cell types. Several hypotheses and works have suggested that the metabolism of resveratrol could be at the origin of a different cellular response. We show here, using colorectal tumor cell lines, that the biological effects of RSV result mainly from its carriage by carriers of the superfamily of ABC transporter, i.e., P-gP, MRP, or BCRP. Using cell lines overexpressing these different transporters, we have been able to highlight the importance of P-gP in the response of cells to RSV. These results were confirmed by invalidating the gene coding for P-gP, which restored the sensitivity of colorectal cells resistant to the polyphenol. Subsequently, the status of P-glycoprotein expression is an important element to be taken into consideration in the cytotoxic activity of resveratrol in colorectal cancer cells. |
format | Online Article Text |
id | pubmed-6770091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67700912019-10-30 P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells Aires, Virginie Colin, Didier J Doreau, Agnès Di Pietro, Attilio Heydel, Jean-Marie Artur, Yves Latruffe, Norbert Delmas, Dominique Nutrients Article Resveratrol has been proposed to prevent tumor growth and the different steps of carcinogenesis; nevertheless, these biological effects are sometimes discordant between different cell types. Several hypotheses and works have suggested that the metabolism of resveratrol could be at the origin of a different cellular response. We show here, using colorectal tumor cell lines, that the biological effects of RSV result mainly from its carriage by carriers of the superfamily of ABC transporter, i.e., P-gP, MRP, or BCRP. Using cell lines overexpressing these different transporters, we have been able to highlight the importance of P-gP in the response of cells to RSV. These results were confirmed by invalidating the gene coding for P-gP, which restored the sensitivity of colorectal cells resistant to the polyphenol. Subsequently, the status of P-glycoprotein expression is an important element to be taken into consideration in the cytotoxic activity of resveratrol in colorectal cancer cells. MDPI 2019-09-04 /pmc/articles/PMC6770091/ /pubmed/31487863 http://dx.doi.org/10.3390/nu11092098 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Aires, Virginie Colin, Didier J Doreau, Agnès Di Pietro, Attilio Heydel, Jean-Marie Artur, Yves Latruffe, Norbert Delmas, Dominique P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells |
title | P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells |
title_full | P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells |
title_fullStr | P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells |
title_full_unstemmed | P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells |
title_short | P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells |
title_sort | p-glycoprotein 1 affects chemoactivities of resveratrol against human colorectal cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770091/ https://www.ncbi.nlm.nih.gov/pubmed/31487863 http://dx.doi.org/10.3390/nu11092098 |
work_keys_str_mv | AT airesvirginie pglycoprotein1affectschemoactivitiesofresveratrolagainsthumancolorectalcancercells AT colindidierj pglycoprotein1affectschemoactivitiesofresveratrolagainsthumancolorectalcancercells AT doreauagnes pglycoprotein1affectschemoactivitiesofresveratrolagainsthumancolorectalcancercells AT dipietroattilio pglycoprotein1affectschemoactivitiesofresveratrolagainsthumancolorectalcancercells AT heydeljeanmarie pglycoprotein1affectschemoactivitiesofresveratrolagainsthumancolorectalcancercells AT arturyves pglycoprotein1affectschemoactivitiesofresveratrolagainsthumancolorectalcancercells AT latruffenorbert pglycoprotein1affectschemoactivitiesofresveratrolagainsthumancolorectalcancercells AT delmasdominique pglycoprotein1affectschemoactivitiesofresveratrolagainsthumancolorectalcancercells |