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P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells

Resveratrol has been proposed to prevent tumor growth and the different steps of carcinogenesis; nevertheless, these biological effects are sometimes discordant between different cell types. Several hypotheses and works have suggested that the metabolism of resveratrol could be at the origin of a di...

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Autores principales: Aires, Virginie, Colin, Didier J, Doreau, Agnès, Di Pietro, Attilio, Heydel, Jean-Marie, Artur, Yves, Latruffe, Norbert, Delmas, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770091/
https://www.ncbi.nlm.nih.gov/pubmed/31487863
http://dx.doi.org/10.3390/nu11092098
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author Aires, Virginie
Colin, Didier J
Doreau, Agnès
Di Pietro, Attilio
Heydel, Jean-Marie
Artur, Yves
Latruffe, Norbert
Delmas, Dominique
author_facet Aires, Virginie
Colin, Didier J
Doreau, Agnès
Di Pietro, Attilio
Heydel, Jean-Marie
Artur, Yves
Latruffe, Norbert
Delmas, Dominique
author_sort Aires, Virginie
collection PubMed
description Resveratrol has been proposed to prevent tumor growth and the different steps of carcinogenesis; nevertheless, these biological effects are sometimes discordant between different cell types. Several hypotheses and works have suggested that the metabolism of resveratrol could be at the origin of a different cellular response. We show here, using colorectal tumor cell lines, that the biological effects of RSV result mainly from its carriage by carriers of the superfamily of ABC transporter, i.e., P-gP, MRP, or BCRP. Using cell lines overexpressing these different transporters, we have been able to highlight the importance of P-gP in the response of cells to RSV. These results were confirmed by invalidating the gene coding for P-gP, which restored the sensitivity of colorectal cells resistant to the polyphenol. Subsequently, the status of P-glycoprotein expression is an important element to be taken into consideration in the cytotoxic activity of resveratrol in colorectal cancer cells.
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spelling pubmed-67700912019-10-30 P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells Aires, Virginie Colin, Didier J Doreau, Agnès Di Pietro, Attilio Heydel, Jean-Marie Artur, Yves Latruffe, Norbert Delmas, Dominique Nutrients Article Resveratrol has been proposed to prevent tumor growth and the different steps of carcinogenesis; nevertheless, these biological effects are sometimes discordant between different cell types. Several hypotheses and works have suggested that the metabolism of resveratrol could be at the origin of a different cellular response. We show here, using colorectal tumor cell lines, that the biological effects of RSV result mainly from its carriage by carriers of the superfamily of ABC transporter, i.e., P-gP, MRP, or BCRP. Using cell lines overexpressing these different transporters, we have been able to highlight the importance of P-gP in the response of cells to RSV. These results were confirmed by invalidating the gene coding for P-gP, which restored the sensitivity of colorectal cells resistant to the polyphenol. Subsequently, the status of P-glycoprotein expression is an important element to be taken into consideration in the cytotoxic activity of resveratrol in colorectal cancer cells. MDPI 2019-09-04 /pmc/articles/PMC6770091/ /pubmed/31487863 http://dx.doi.org/10.3390/nu11092098 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aires, Virginie
Colin, Didier J
Doreau, Agnès
Di Pietro, Attilio
Heydel, Jean-Marie
Artur, Yves
Latruffe, Norbert
Delmas, Dominique
P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells
title P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells
title_full P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells
title_fullStr P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells
title_full_unstemmed P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells
title_short P-Glycoprotein 1 Affects Chemoactivities of Resveratrol against Human Colorectal Cancer Cells
title_sort p-glycoprotein 1 affects chemoactivities of resveratrol against human colorectal cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770091/
https://www.ncbi.nlm.nih.gov/pubmed/31487863
http://dx.doi.org/10.3390/nu11092098
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