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How the 26S Proteasome Degrades Ubiquitinated Proteins in the Cell
The 26S proteasome is the central element of proteostasis regulation in eukaryotic cells, it is required for the degradation of protein factors in multiple cellular pathways and it plays a fundamental role in cell stability. The main aspects of proteasome mediated protein degradation have been highl...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770211/ https://www.ncbi.nlm.nih.gov/pubmed/31443414 http://dx.doi.org/10.3390/biom9090395 |
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author | Coll-Martínez, Bernat Crosas, Bernat |
author_facet | Coll-Martínez, Bernat Crosas, Bernat |
author_sort | Coll-Martínez, Bernat |
collection | PubMed |
description | The 26S proteasome is the central element of proteostasis regulation in eukaryotic cells, it is required for the degradation of protein factors in multiple cellular pathways and it plays a fundamental role in cell stability. The main aspects of proteasome mediated protein degradation have been highly (but not totally) described during three decades of intense cellular, molecular, structural and chemical biology research and tool development. Contributions accumulated within this time lapse allow researchers today to go beyond classical partial views of the pathway, and start generating almost complete views of how the proteasome acts inside the cell. These views have been recently reinforced by cryo-electron microscopy and mechanistic works that provide from landscapes of proteasomal populations distributed in distinct intracellular contexts, to detailed shots of each step of the process of degradation of a given substrate, of the factors that regulate it, and precise measurements of the speed of degradation. Here, we present an updated digest of the most recent developments that significantly contribute in our understanding of how the 26S proteasome degrades hundreds of ubiquitinated substrates in multiple intracellular environments. |
format | Online Article Text |
id | pubmed-6770211 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67702112019-10-30 How the 26S Proteasome Degrades Ubiquitinated Proteins in the Cell Coll-Martínez, Bernat Crosas, Bernat Biomolecules Review The 26S proteasome is the central element of proteostasis regulation in eukaryotic cells, it is required for the degradation of protein factors in multiple cellular pathways and it plays a fundamental role in cell stability. The main aspects of proteasome mediated protein degradation have been highly (but not totally) described during three decades of intense cellular, molecular, structural and chemical biology research and tool development. Contributions accumulated within this time lapse allow researchers today to go beyond classical partial views of the pathway, and start generating almost complete views of how the proteasome acts inside the cell. These views have been recently reinforced by cryo-electron microscopy and mechanistic works that provide from landscapes of proteasomal populations distributed in distinct intracellular contexts, to detailed shots of each step of the process of degradation of a given substrate, of the factors that regulate it, and precise measurements of the speed of degradation. Here, we present an updated digest of the most recent developments that significantly contribute in our understanding of how the 26S proteasome degrades hundreds of ubiquitinated substrates in multiple intracellular environments. MDPI 2019-08-22 /pmc/articles/PMC6770211/ /pubmed/31443414 http://dx.doi.org/10.3390/biom9090395 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Coll-Martínez, Bernat Crosas, Bernat How the 26S Proteasome Degrades Ubiquitinated Proteins in the Cell |
title | How the 26S Proteasome Degrades Ubiquitinated Proteins in the Cell |
title_full | How the 26S Proteasome Degrades Ubiquitinated Proteins in the Cell |
title_fullStr | How the 26S Proteasome Degrades Ubiquitinated Proteins in the Cell |
title_full_unstemmed | How the 26S Proteasome Degrades Ubiquitinated Proteins in the Cell |
title_short | How the 26S Proteasome Degrades Ubiquitinated Proteins in the Cell |
title_sort | how the 26s proteasome degrades ubiquitinated proteins in the cell |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770211/ https://www.ncbi.nlm.nih.gov/pubmed/31443414 http://dx.doi.org/10.3390/biom9090395 |
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