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Genome-Wide Analysis Reveals the Role of Mediator Complex in the Soybean—Phytophthora sojae Interaction
The mediator complex is an essential link between transcription factors and RNA polymerase II, and mainly functions in the transduction of diverse signals to genes involved in different pathways. Limited information is available on the role of soybean mediator subunits in growth and development, and...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770253/ https://www.ncbi.nlm.nih.gov/pubmed/31540158 http://dx.doi.org/10.3390/ijms20184570 |
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author | Xue, Dong Guo, Na Zhang, Xiao-Li Zhao, Jin-Ming Bu, Yuan-Peng Jiang, Dian-Liang Wang, Xiao-Ting Wang, Hai-Tang Guan, Rong-Zhan Xing, Han |
author_facet | Xue, Dong Guo, Na Zhang, Xiao-Li Zhao, Jin-Ming Bu, Yuan-Peng Jiang, Dian-Liang Wang, Xiao-Ting Wang, Hai-Tang Guan, Rong-Zhan Xing, Han |
author_sort | Xue, Dong |
collection | PubMed |
description | The mediator complex is an essential link between transcription factors and RNA polymerase II, and mainly functions in the transduction of diverse signals to genes involved in different pathways. Limited information is available on the role of soybean mediator subunits in growth and development, and their participation in defense response regulation. Here, we performed genome-wide identification of the 95 soybean mediator subunits, which were unevenly localized on the 20 chromosomes and only segmental duplication events were detected. We focused on GmMED16-1, which is highly expressed in the roots, for further functional analysis. Transcription of GmMED16-1 was induced in response to Phytophthora sojae infection. Agrobacterium rhizogenes mediated soybean hairy root transformation was performed for the silencing of the GmMED16-1 gene. Silencing of GmMED16-1 led to an enhanced susceptibility phenotype and increased accumulation of P. sojae biomass in hairy roots of transformants. The transcript levels of NPR1, PR1a, and PR5 in the salicylic acid defense pathway in roots of GmMED16-1-silenced transformants were lower than those of empty-vector transformants. The results provide evidence that GmMED16-1 may participate in the soybean–P. sojae interaction via a salicylic acid-dependent process. |
format | Online Article Text |
id | pubmed-6770253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67702532019-10-30 Genome-Wide Analysis Reveals the Role of Mediator Complex in the Soybean—Phytophthora sojae Interaction Xue, Dong Guo, Na Zhang, Xiao-Li Zhao, Jin-Ming Bu, Yuan-Peng Jiang, Dian-Liang Wang, Xiao-Ting Wang, Hai-Tang Guan, Rong-Zhan Xing, Han Int J Mol Sci Article The mediator complex is an essential link between transcription factors and RNA polymerase II, and mainly functions in the transduction of diverse signals to genes involved in different pathways. Limited information is available on the role of soybean mediator subunits in growth and development, and their participation in defense response regulation. Here, we performed genome-wide identification of the 95 soybean mediator subunits, which were unevenly localized on the 20 chromosomes and only segmental duplication events were detected. We focused on GmMED16-1, which is highly expressed in the roots, for further functional analysis. Transcription of GmMED16-1 was induced in response to Phytophthora sojae infection. Agrobacterium rhizogenes mediated soybean hairy root transformation was performed for the silencing of the GmMED16-1 gene. Silencing of GmMED16-1 led to an enhanced susceptibility phenotype and increased accumulation of P. sojae biomass in hairy roots of transformants. The transcript levels of NPR1, PR1a, and PR5 in the salicylic acid defense pathway in roots of GmMED16-1-silenced transformants were lower than those of empty-vector transformants. The results provide evidence that GmMED16-1 may participate in the soybean–P. sojae interaction via a salicylic acid-dependent process. MDPI 2019-09-15 /pmc/articles/PMC6770253/ /pubmed/31540158 http://dx.doi.org/10.3390/ijms20184570 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xue, Dong Guo, Na Zhang, Xiao-Li Zhao, Jin-Ming Bu, Yuan-Peng Jiang, Dian-Liang Wang, Xiao-Ting Wang, Hai-Tang Guan, Rong-Zhan Xing, Han Genome-Wide Analysis Reveals the Role of Mediator Complex in the Soybean—Phytophthora sojae Interaction |
title | Genome-Wide Analysis Reveals the Role of Mediator Complex in the Soybean—Phytophthora sojae Interaction |
title_full | Genome-Wide Analysis Reveals the Role of Mediator Complex in the Soybean—Phytophthora sojae Interaction |
title_fullStr | Genome-Wide Analysis Reveals the Role of Mediator Complex in the Soybean—Phytophthora sojae Interaction |
title_full_unstemmed | Genome-Wide Analysis Reveals the Role of Mediator Complex in the Soybean—Phytophthora sojae Interaction |
title_short | Genome-Wide Analysis Reveals the Role of Mediator Complex in the Soybean—Phytophthora sojae Interaction |
title_sort | genome-wide analysis reveals the role of mediator complex in the soybean—phytophthora sojae interaction |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770253/ https://www.ncbi.nlm.nih.gov/pubmed/31540158 http://dx.doi.org/10.3390/ijms20184570 |
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