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Lipid Profiling Reveals Browning Heterogeneity of White Adipose Tissue by Β3-Adrenergic Stimulation

Background: White adipose tissue (WAT) browning confers beneficial effects on metabolic diseases. However, visceral adipose tissue (VAT) is not as susceptible to browning as subcutaneous adipose tissue (SAT). Aim: Interpreting the heterogeneity of VAT and SAT in brown remodeling and provide promisin...

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Autores principales: He, Ping, Hou, Biyu, Li, Yanliang, Xu, Chunyang, Ma, Peng, Lam, Sin Man, Gil, Victoria, Yang, Xinyu, Yang, Xiuying, Zhang, Li, Shui, Guanghou, Song, Junke, Qiang, Guifen, Liew, Chong Wee, Du, Guanhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770315/
https://www.ncbi.nlm.nih.gov/pubmed/31484405
http://dx.doi.org/10.3390/biom9090444
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author He, Ping
Hou, Biyu
Li, Yanliang
Xu, Chunyang
Ma, Peng
Lam, Sin Man
Gil, Victoria
Yang, Xinyu
Yang, Xiuying
Zhang, Li
Shui, Guanghou
Song, Junke
Qiang, Guifen
Liew, Chong Wee
Du, Guanhua
author_facet He, Ping
Hou, Biyu
Li, Yanliang
Xu, Chunyang
Ma, Peng
Lam, Sin Man
Gil, Victoria
Yang, Xinyu
Yang, Xiuying
Zhang, Li
Shui, Guanghou
Song, Junke
Qiang, Guifen
Liew, Chong Wee
Du, Guanhua
author_sort He, Ping
collection PubMed
description Background: White adipose tissue (WAT) browning confers beneficial effects on metabolic diseases. However, visceral adipose tissue (VAT) is not as susceptible to browning as subcutaneous adipose tissue (SAT). Aim: Interpreting the heterogeneity of VAT and SAT in brown remodeling and provide promising lipid targets to promote WAT browning. Methods: We first investigated the effects of β3-adrenergic stimulation by CL316,243 on systemic metabolism. Then, high-coverage targeted lipidomics approach with multiple reaction monitoring (MRM) was utilized to provide extensive detection of lipid metabolites in VAT and SAT. Results: CL316,243 notably ameliorated the systemic metabolism and induced brown remodeling of SAT but browning resistance of VAT. Comprehensive lipidomics analysis revealed browning heterogeneity of VAT and SAT with more dramatic alteration of lipid classes and species in VAT rather than SAT, though VAT is resistant to browning. Adrenergic stimulation differentially affected glycerides content in VAT and SAT and boosted the abundance of more glycerophospholipids species in VAT than in SAT. Besides, CL316,243 increased sphingolipids in VAT without changes in SAT, meanwhile, elevated cardiolipin species more prominently in VAT than in SAT. Conclusions: We demonstrated the browning heterogeneity of WAT and identified potential lipid biomarkers which may provide lipid targets for overcoming VAT browning resistance.
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spelling pubmed-67703152019-10-30 Lipid Profiling Reveals Browning Heterogeneity of White Adipose Tissue by Β3-Adrenergic Stimulation He, Ping Hou, Biyu Li, Yanliang Xu, Chunyang Ma, Peng Lam, Sin Man Gil, Victoria Yang, Xinyu Yang, Xiuying Zhang, Li Shui, Guanghou Song, Junke Qiang, Guifen Liew, Chong Wee Du, Guanhua Biomolecules Article Background: White adipose tissue (WAT) browning confers beneficial effects on metabolic diseases. However, visceral adipose tissue (VAT) is not as susceptible to browning as subcutaneous adipose tissue (SAT). Aim: Interpreting the heterogeneity of VAT and SAT in brown remodeling and provide promising lipid targets to promote WAT browning. Methods: We first investigated the effects of β3-adrenergic stimulation by CL316,243 on systemic metabolism. Then, high-coverage targeted lipidomics approach with multiple reaction monitoring (MRM) was utilized to provide extensive detection of lipid metabolites in VAT and SAT. Results: CL316,243 notably ameliorated the systemic metabolism and induced brown remodeling of SAT but browning resistance of VAT. Comprehensive lipidomics analysis revealed browning heterogeneity of VAT and SAT with more dramatic alteration of lipid classes and species in VAT rather than SAT, though VAT is resistant to browning. Adrenergic stimulation differentially affected glycerides content in VAT and SAT and boosted the abundance of more glycerophospholipids species in VAT than in SAT. Besides, CL316,243 increased sphingolipids in VAT without changes in SAT, meanwhile, elevated cardiolipin species more prominently in VAT than in SAT. Conclusions: We demonstrated the browning heterogeneity of WAT and identified potential lipid biomarkers which may provide lipid targets for overcoming VAT browning resistance. MDPI 2019-09-03 /pmc/articles/PMC6770315/ /pubmed/31484405 http://dx.doi.org/10.3390/biom9090444 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
He, Ping
Hou, Biyu
Li, Yanliang
Xu, Chunyang
Ma, Peng
Lam, Sin Man
Gil, Victoria
Yang, Xinyu
Yang, Xiuying
Zhang, Li
Shui, Guanghou
Song, Junke
Qiang, Guifen
Liew, Chong Wee
Du, Guanhua
Lipid Profiling Reveals Browning Heterogeneity of White Adipose Tissue by Β3-Adrenergic Stimulation
title Lipid Profiling Reveals Browning Heterogeneity of White Adipose Tissue by Β3-Adrenergic Stimulation
title_full Lipid Profiling Reveals Browning Heterogeneity of White Adipose Tissue by Β3-Adrenergic Stimulation
title_fullStr Lipid Profiling Reveals Browning Heterogeneity of White Adipose Tissue by Β3-Adrenergic Stimulation
title_full_unstemmed Lipid Profiling Reveals Browning Heterogeneity of White Adipose Tissue by Β3-Adrenergic Stimulation
title_short Lipid Profiling Reveals Browning Heterogeneity of White Adipose Tissue by Β3-Adrenergic Stimulation
title_sort lipid profiling reveals browning heterogeneity of white adipose tissue by β3-adrenergic stimulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770315/
https://www.ncbi.nlm.nih.gov/pubmed/31484405
http://dx.doi.org/10.3390/biom9090444
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