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The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas

Our previous research revealed that trehalose, a nonreducing disaccharide of glucose and an important stress responsive factor, proved to have anti-inflammatory, antiapoptotic, and particularly antioxidant properties in UVB-irradiated corneas. Trehalose reduced oxidative stress in corneas induced by...

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Autores principales: Cejka, Cestmir, Kossl, Jan, Hermankova, Barbora, Holan, Vladimir, Kubinova, Sarka, Olmiere, Céline, Cejkova, Jitka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770344/
https://www.ncbi.nlm.nih.gov/pubmed/31641422
http://dx.doi.org/10.1155/2019/1857086
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author Cejka, Cestmir
Kossl, Jan
Hermankova, Barbora
Holan, Vladimir
Kubinova, Sarka
Olmiere, Céline
Cejkova, Jitka
author_facet Cejka, Cestmir
Kossl, Jan
Hermankova, Barbora
Holan, Vladimir
Kubinova, Sarka
Olmiere, Céline
Cejkova, Jitka
author_sort Cejka, Cestmir
collection PubMed
description Our previous research revealed that trehalose, a nonreducing disaccharide of glucose and an important stress responsive factor, proved to have anti-inflammatory, antiapoptotic, and particularly antioxidant properties in UVB-irradiated corneas. Trehalose reduced oxidative stress in corneas induced by UVB irradiation, by means of a decrease in the antioxidant/prooxidant imbalance in the corneal epithelium. In this study, we demonstrate that trehalose of 3% or 6% concentration in eye drops directly decreases oxidative stress in UVB-irradiated corneas, by removing the excessive amount of reactive oxygen species (ROS). Trehalose drops applied on corneas during UVB irradiation once daily for four days resulted in a reduction or even absence of the oxidative stress, DNA damage, and peroxynitrite formation (detected by nitrotyrosine residues), seen in buffer-treated corneas. Furthermore, trehalose treatment applied curatively after repeated irradiation for the subsequent fourteen days led to the renewal of corneal transparency and significant suppression or even absence of neovascularization. This was in contrast to buffer-treated irradiated corneas, where the intracorneal inflammation was developed and the untransparent corneas were vascularized. In conclusion, the treatment of UVB-irradiated corneas with trehalose eye drops removed the excessive amount of ROS in the corneal epithelium, leading to the suppression of oxidative stress and favorable corneal healing. The 6% trehalose showed a higher intensive antioxidant effect.
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spelling pubmed-67703442019-10-22 The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas Cejka, Cestmir Kossl, Jan Hermankova, Barbora Holan, Vladimir Kubinova, Sarka Olmiere, Céline Cejkova, Jitka Oxid Med Cell Longev Research Article Our previous research revealed that trehalose, a nonreducing disaccharide of glucose and an important stress responsive factor, proved to have anti-inflammatory, antiapoptotic, and particularly antioxidant properties in UVB-irradiated corneas. Trehalose reduced oxidative stress in corneas induced by UVB irradiation, by means of a decrease in the antioxidant/prooxidant imbalance in the corneal epithelium. In this study, we demonstrate that trehalose of 3% or 6% concentration in eye drops directly decreases oxidative stress in UVB-irradiated corneas, by removing the excessive amount of reactive oxygen species (ROS). Trehalose drops applied on corneas during UVB irradiation once daily for four days resulted in a reduction or even absence of the oxidative stress, DNA damage, and peroxynitrite formation (detected by nitrotyrosine residues), seen in buffer-treated corneas. Furthermore, trehalose treatment applied curatively after repeated irradiation for the subsequent fourteen days led to the renewal of corneal transparency and significant suppression or even absence of neovascularization. This was in contrast to buffer-treated irradiated corneas, where the intracorneal inflammation was developed and the untransparent corneas were vascularized. In conclusion, the treatment of UVB-irradiated corneas with trehalose eye drops removed the excessive amount of ROS in the corneal epithelium, leading to the suppression of oxidative stress and favorable corneal healing. The 6% trehalose showed a higher intensive antioxidant effect. Hindawi 2019-09-19 /pmc/articles/PMC6770344/ /pubmed/31641422 http://dx.doi.org/10.1155/2019/1857086 Text en Copyright © 2019 Cestmir Cejka et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cejka, Cestmir
Kossl, Jan
Hermankova, Barbora
Holan, Vladimir
Kubinova, Sarka
Olmiere, Céline
Cejkova, Jitka
The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas
title The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas
title_full The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas
title_fullStr The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas
title_full_unstemmed The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas
title_short The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas
title_sort healing of oxidative injuries with trehalose in uvb-irradiated rabbit corneas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770344/
https://www.ncbi.nlm.nih.gov/pubmed/31641422
http://dx.doi.org/10.1155/2019/1857086
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