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Acute/Subacute and Sub-Chronic Oral Toxicity of a Hidroxytyrosol-Rich Virgin Olive Oil Extract

The objective of this study was to determine the acute (one single dose), subacute (14 days), and sub-chronic (90 days) toxicity of an aqueous virgin olive oil (VOO) extract rich in hydroxytyrosol in rats. For acute/subacute toxicity, rats were divided into three groups. The control group received d...

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Autores principales: Rodríguez-Lara, Avilene, Mesa, María Dolores, Aragón-Vela, Jerónimo, Casuso, Rafael A., Casals Vázquez, Cristina, Zúñiga, Jesús M., Huertas, Jesús R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770357/
https://www.ncbi.nlm.nih.gov/pubmed/31500145
http://dx.doi.org/10.3390/nu11092133
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author Rodríguez-Lara, Avilene
Mesa, María Dolores
Aragón-Vela, Jerónimo
Casuso, Rafael A.
Casals Vázquez, Cristina
Zúñiga, Jesús M.
Huertas, Jesús R.
author_facet Rodríguez-Lara, Avilene
Mesa, María Dolores
Aragón-Vela, Jerónimo
Casuso, Rafael A.
Casals Vázquez, Cristina
Zúñiga, Jesús M.
Huertas, Jesús R.
author_sort Rodríguez-Lara, Avilene
collection PubMed
description The objective of this study was to determine the acute (one single dose), subacute (14 days), and sub-chronic (90 days) toxicity of an aqueous virgin olive oil (VOO) extract rich in hydroxytyrosol in rats. For acute/subacute toxicity, rats were divided into three groups. The control group received distilled water (n = 9), another experimental group received a single dose of 300 mg/kg (n = 3), and a third group received one dose of 2000 mg/kg (n = 4) during 14 days. The sub-chronic study included 60rats distributed in three groups (n = 20: 10 males and 10 females) receiving daily different three doses of the VOO extract in the drinking water during 90 days: (1) 100 mg/kg, (2) 300 mg/kg, and (3) 1000 mg/kg. In parallel, a fourth additional group (n = 20: 10 males and 10 females) did not receive any extract (control group). Clinical signs, body weight, functional observations of sensory and motor reactivity, hematological and biochemical analyses, and macroscopic and microscopic histopathology were evaluated. No adverse effects were observed after the administration of the different doses of the hydroxytyrosol-rich VOO extract, which suggests that the enrichment of VOO in its phenolic compound is safe, and can be used as functional foods for the treatment of chronic degenerative diseases.
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spelling pubmed-67703572019-10-30 Acute/Subacute and Sub-Chronic Oral Toxicity of a Hidroxytyrosol-Rich Virgin Olive Oil Extract Rodríguez-Lara, Avilene Mesa, María Dolores Aragón-Vela, Jerónimo Casuso, Rafael A. Casals Vázquez, Cristina Zúñiga, Jesús M. Huertas, Jesús R. Nutrients Article The objective of this study was to determine the acute (one single dose), subacute (14 days), and sub-chronic (90 days) toxicity of an aqueous virgin olive oil (VOO) extract rich in hydroxytyrosol in rats. For acute/subacute toxicity, rats were divided into three groups. The control group received distilled water (n = 9), another experimental group received a single dose of 300 mg/kg (n = 3), and a third group received one dose of 2000 mg/kg (n = 4) during 14 days. The sub-chronic study included 60rats distributed in three groups (n = 20: 10 males and 10 females) receiving daily different three doses of the VOO extract in the drinking water during 90 days: (1) 100 mg/kg, (2) 300 mg/kg, and (3) 1000 mg/kg. In parallel, a fourth additional group (n = 20: 10 males and 10 females) did not receive any extract (control group). Clinical signs, body weight, functional observations of sensory and motor reactivity, hematological and biochemical analyses, and macroscopic and microscopic histopathology were evaluated. No adverse effects were observed after the administration of the different doses of the hydroxytyrosol-rich VOO extract, which suggests that the enrichment of VOO in its phenolic compound is safe, and can be used as functional foods for the treatment of chronic degenerative diseases. MDPI 2019-09-06 /pmc/articles/PMC6770357/ /pubmed/31500145 http://dx.doi.org/10.3390/nu11092133 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rodríguez-Lara, Avilene
Mesa, María Dolores
Aragón-Vela, Jerónimo
Casuso, Rafael A.
Casals Vázquez, Cristina
Zúñiga, Jesús M.
Huertas, Jesús R.
Acute/Subacute and Sub-Chronic Oral Toxicity of a Hidroxytyrosol-Rich Virgin Olive Oil Extract
title Acute/Subacute and Sub-Chronic Oral Toxicity of a Hidroxytyrosol-Rich Virgin Olive Oil Extract
title_full Acute/Subacute and Sub-Chronic Oral Toxicity of a Hidroxytyrosol-Rich Virgin Olive Oil Extract
title_fullStr Acute/Subacute and Sub-Chronic Oral Toxicity of a Hidroxytyrosol-Rich Virgin Olive Oil Extract
title_full_unstemmed Acute/Subacute and Sub-Chronic Oral Toxicity of a Hidroxytyrosol-Rich Virgin Olive Oil Extract
title_short Acute/Subacute and Sub-Chronic Oral Toxicity of a Hidroxytyrosol-Rich Virgin Olive Oil Extract
title_sort acute/subacute and sub-chronic oral toxicity of a hidroxytyrosol-rich virgin olive oil extract
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770357/
https://www.ncbi.nlm.nih.gov/pubmed/31500145
http://dx.doi.org/10.3390/nu11092133
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