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Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome

Aim: We aimed to examine the alterations of the insulin signaling pathway, autophagy, nitrative stress and the effect of vitamin D supplementation in the liver and ovaries of vitamin D deficient hyperandrogenic rats. Methods: Female Wistar rats received eight weeks of transdermal testosterone treatm...

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Autores principales: Lajtai, Krisztina, Nagy, Csilla Terézia, Tarszabó, Róbert, Benkő, Rita, Hadjadj, Leila, Sziva, Réka Eszter, Gerszi, Dóra, Bányai, Bálint, Ferdinandy, Péter, Nádasy, György László, Giricz, Zoltán, Horváth, Eszter Mária, Várbíró, Szabolcs
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770417/
https://www.ncbi.nlm.nih.gov/pubmed/31509973
http://dx.doi.org/10.3390/biom9090471
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author Lajtai, Krisztina
Nagy, Csilla Terézia
Tarszabó, Róbert
Benkő, Rita
Hadjadj, Leila
Sziva, Réka Eszter
Gerszi, Dóra
Bányai, Bálint
Ferdinandy, Péter
Nádasy, György László
Giricz, Zoltán
Horváth, Eszter Mária
Várbíró, Szabolcs
author_facet Lajtai, Krisztina
Nagy, Csilla Terézia
Tarszabó, Róbert
Benkő, Rita
Hadjadj, Leila
Sziva, Réka Eszter
Gerszi, Dóra
Bányai, Bálint
Ferdinandy, Péter
Nádasy, György László
Giricz, Zoltán
Horváth, Eszter Mária
Várbíró, Szabolcs
author_sort Lajtai, Krisztina
collection PubMed
description Aim: We aimed to examine the alterations of the insulin signaling pathway, autophagy, nitrative stress and the effect of vitamin D supplementation in the liver and ovaries of vitamin D deficient hyperandrogenic rats. Methods: Female Wistar rats received eight weeks of transdermal testosterone treatment and lived on a low vitamin D diet (D–T+). Vitamin D supplementation was achieved by oral administration of vitamin D3 (D+T+). Sham-treated (D+T–) and vitamin D deficient animals (D–T–) served as controls. (N = 10–12 per group). Results: D–T+ animals showed decreased LC3 II levels in the liver and increased p-Akt/Akt and p-eNOS/eNOS ratios with decreased insulin receptor staining in the ovaries. Vitamin D supplementation prevented the increase of Akt phosphorylation in the ovaries. Vitamin D deficiency itself also led to decreased LC3 II levels in the liver and decreased insulin receptor staining in the ovaries. D–T+ group showed no increase in nitrotyrosine staining; however, the ovaries of D–T– rats and the liver of D+T+ animals showed increased staining intensity. Conclusion: Vitamin D deficiency itself might lead to disrupted ovarian maturation and autophagy malfunction in the liver. Preventing Akt phosphorylation may contribute to the beneficial effect of vitamin D treatment on ovarian function in hyperandrogenism.
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spelling pubmed-67704172019-10-30 Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome Lajtai, Krisztina Nagy, Csilla Terézia Tarszabó, Róbert Benkő, Rita Hadjadj, Leila Sziva, Réka Eszter Gerszi, Dóra Bányai, Bálint Ferdinandy, Péter Nádasy, György László Giricz, Zoltán Horváth, Eszter Mária Várbíró, Szabolcs Biomolecules Article Aim: We aimed to examine the alterations of the insulin signaling pathway, autophagy, nitrative stress and the effect of vitamin D supplementation in the liver and ovaries of vitamin D deficient hyperandrogenic rats. Methods: Female Wistar rats received eight weeks of transdermal testosterone treatment and lived on a low vitamin D diet (D–T+). Vitamin D supplementation was achieved by oral administration of vitamin D3 (D+T+). Sham-treated (D+T–) and vitamin D deficient animals (D–T–) served as controls. (N = 10–12 per group). Results: D–T+ animals showed decreased LC3 II levels in the liver and increased p-Akt/Akt and p-eNOS/eNOS ratios with decreased insulin receptor staining in the ovaries. Vitamin D supplementation prevented the increase of Akt phosphorylation in the ovaries. Vitamin D deficiency itself also led to decreased LC3 II levels in the liver and decreased insulin receptor staining in the ovaries. D–T+ group showed no increase in nitrotyrosine staining; however, the ovaries of D–T– rats and the liver of D+T+ animals showed increased staining intensity. Conclusion: Vitamin D deficiency itself might lead to disrupted ovarian maturation and autophagy malfunction in the liver. Preventing Akt phosphorylation may contribute to the beneficial effect of vitamin D treatment on ovarian function in hyperandrogenism. MDPI 2019-09-10 /pmc/articles/PMC6770417/ /pubmed/31509973 http://dx.doi.org/10.3390/biom9090471 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lajtai, Krisztina
Nagy, Csilla Terézia
Tarszabó, Róbert
Benkő, Rita
Hadjadj, Leila
Sziva, Réka Eszter
Gerszi, Dóra
Bányai, Bálint
Ferdinandy, Péter
Nádasy, György László
Giricz, Zoltán
Horváth, Eszter Mária
Várbíró, Szabolcs
Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome
title Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome
title_full Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome
title_fullStr Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome
title_full_unstemmed Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome
title_short Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome
title_sort effects of vitamin d deficiency on proliferation and autophagy of ovarian and liver tissues in a rat model of polycystic ovary syndrome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770417/
https://www.ncbi.nlm.nih.gov/pubmed/31509973
http://dx.doi.org/10.3390/biom9090471
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