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Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome
Aim: We aimed to examine the alterations of the insulin signaling pathway, autophagy, nitrative stress and the effect of vitamin D supplementation in the liver and ovaries of vitamin D deficient hyperandrogenic rats. Methods: Female Wistar rats received eight weeks of transdermal testosterone treatm...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770417/ https://www.ncbi.nlm.nih.gov/pubmed/31509973 http://dx.doi.org/10.3390/biom9090471 |
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author | Lajtai, Krisztina Nagy, Csilla Terézia Tarszabó, Róbert Benkő, Rita Hadjadj, Leila Sziva, Réka Eszter Gerszi, Dóra Bányai, Bálint Ferdinandy, Péter Nádasy, György László Giricz, Zoltán Horváth, Eszter Mária Várbíró, Szabolcs |
author_facet | Lajtai, Krisztina Nagy, Csilla Terézia Tarszabó, Róbert Benkő, Rita Hadjadj, Leila Sziva, Réka Eszter Gerszi, Dóra Bányai, Bálint Ferdinandy, Péter Nádasy, György László Giricz, Zoltán Horváth, Eszter Mária Várbíró, Szabolcs |
author_sort | Lajtai, Krisztina |
collection | PubMed |
description | Aim: We aimed to examine the alterations of the insulin signaling pathway, autophagy, nitrative stress and the effect of vitamin D supplementation in the liver and ovaries of vitamin D deficient hyperandrogenic rats. Methods: Female Wistar rats received eight weeks of transdermal testosterone treatment and lived on a low vitamin D diet (D–T+). Vitamin D supplementation was achieved by oral administration of vitamin D3 (D+T+). Sham-treated (D+T–) and vitamin D deficient animals (D–T–) served as controls. (N = 10–12 per group). Results: D–T+ animals showed decreased LC3 II levels in the liver and increased p-Akt/Akt and p-eNOS/eNOS ratios with decreased insulin receptor staining in the ovaries. Vitamin D supplementation prevented the increase of Akt phosphorylation in the ovaries. Vitamin D deficiency itself also led to decreased LC3 II levels in the liver and decreased insulin receptor staining in the ovaries. D–T+ group showed no increase in nitrotyrosine staining; however, the ovaries of D–T– rats and the liver of D+T+ animals showed increased staining intensity. Conclusion: Vitamin D deficiency itself might lead to disrupted ovarian maturation and autophagy malfunction in the liver. Preventing Akt phosphorylation may contribute to the beneficial effect of vitamin D treatment on ovarian function in hyperandrogenism. |
format | Online Article Text |
id | pubmed-6770417 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67704172019-10-30 Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome Lajtai, Krisztina Nagy, Csilla Terézia Tarszabó, Róbert Benkő, Rita Hadjadj, Leila Sziva, Réka Eszter Gerszi, Dóra Bányai, Bálint Ferdinandy, Péter Nádasy, György László Giricz, Zoltán Horváth, Eszter Mária Várbíró, Szabolcs Biomolecules Article Aim: We aimed to examine the alterations of the insulin signaling pathway, autophagy, nitrative stress and the effect of vitamin D supplementation in the liver and ovaries of vitamin D deficient hyperandrogenic rats. Methods: Female Wistar rats received eight weeks of transdermal testosterone treatment and lived on a low vitamin D diet (D–T+). Vitamin D supplementation was achieved by oral administration of vitamin D3 (D+T+). Sham-treated (D+T–) and vitamin D deficient animals (D–T–) served as controls. (N = 10–12 per group). Results: D–T+ animals showed decreased LC3 II levels in the liver and increased p-Akt/Akt and p-eNOS/eNOS ratios with decreased insulin receptor staining in the ovaries. Vitamin D supplementation prevented the increase of Akt phosphorylation in the ovaries. Vitamin D deficiency itself also led to decreased LC3 II levels in the liver and decreased insulin receptor staining in the ovaries. D–T+ group showed no increase in nitrotyrosine staining; however, the ovaries of D–T– rats and the liver of D+T+ animals showed increased staining intensity. Conclusion: Vitamin D deficiency itself might lead to disrupted ovarian maturation and autophagy malfunction in the liver. Preventing Akt phosphorylation may contribute to the beneficial effect of vitamin D treatment on ovarian function in hyperandrogenism. MDPI 2019-09-10 /pmc/articles/PMC6770417/ /pubmed/31509973 http://dx.doi.org/10.3390/biom9090471 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lajtai, Krisztina Nagy, Csilla Terézia Tarszabó, Róbert Benkő, Rita Hadjadj, Leila Sziva, Réka Eszter Gerszi, Dóra Bányai, Bálint Ferdinandy, Péter Nádasy, György László Giricz, Zoltán Horváth, Eszter Mária Várbíró, Szabolcs Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome |
title | Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome |
title_full | Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome |
title_fullStr | Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome |
title_full_unstemmed | Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome |
title_short | Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome |
title_sort | effects of vitamin d deficiency on proliferation and autophagy of ovarian and liver tissues in a rat model of polycystic ovary syndrome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770417/ https://www.ncbi.nlm.nih.gov/pubmed/31509973 http://dx.doi.org/10.3390/biom9090471 |
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