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Total DNA Methylation Changes Reflect Random Oxidative DNA Damage in Gliomas
DNA modifications can be used to monitor pathological processes. We have previously shown that estimating the amount of the main DNA epigenetic mark, 5-methylcytosine (m(5)C), is an efficient and reliable way to diagnose brain tumors, hypertension, and other diseases. Abnormal increases of reactive...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770701/ https://www.ncbi.nlm.nih.gov/pubmed/31514401 http://dx.doi.org/10.3390/cells8091065 |
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author | Barciszewska, Anna-Maria Giel-Pietraszuk, Małgorzata Perrigue, Patrick M. Naskręt-Barciszewska, Mirosława |
author_facet | Barciszewska, Anna-Maria Giel-Pietraszuk, Małgorzata Perrigue, Patrick M. Naskręt-Barciszewska, Mirosława |
author_sort | Barciszewska, Anna-Maria |
collection | PubMed |
description | DNA modifications can be used to monitor pathological processes. We have previously shown that estimating the amount of the main DNA epigenetic mark, 5-methylcytosine (m(5)C), is an efficient and reliable way to diagnose brain tumors, hypertension, and other diseases. Abnormal increases of reactive oxygen species (ROS) are a driving factor for mutations that lead to changes in m(5)C levels and cancer evolution. 8-oxo-deoxyguanosine (8-oxo-dG) is a specific marker of ROS-driven DNA-damage, and its accumulation makes m(5)C a hotspot for mutations. It is unknown how m(5)C and 8-oxo-dG correlate with the malignancy of gliomas. We analyzed the total contents of m(5)C and 8-oxo-dG in DNA from tumor tissue and peripheral blood samples from brain glioma patients. We found an opposite relationship in the amounts of m(5)C and 8-oxo-dG, which correlated with glioma grade in the way that low level of m(5)C and high level of 8-oxo-dG indicated increased glioma malignancy grade. Our results could be directly applied to patient monitoring and treatment protocols for gliomas, as well as bolster previous findings, suggesting that spontaneously generated ROS react with m(5)C. Because of the similar mechanisms of m(5)C and guanosine oxidation, we concluded that 8-oxo-dG could also predict glioma malignancy grade and global DNA demethylation in cancer cells. |
format | Online Article Text |
id | pubmed-6770701 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67707012019-10-30 Total DNA Methylation Changes Reflect Random Oxidative DNA Damage in Gliomas Barciszewska, Anna-Maria Giel-Pietraszuk, Małgorzata Perrigue, Patrick M. Naskręt-Barciszewska, Mirosława Cells Article DNA modifications can be used to monitor pathological processes. We have previously shown that estimating the amount of the main DNA epigenetic mark, 5-methylcytosine (m(5)C), is an efficient and reliable way to diagnose brain tumors, hypertension, and other diseases. Abnormal increases of reactive oxygen species (ROS) are a driving factor for mutations that lead to changes in m(5)C levels and cancer evolution. 8-oxo-deoxyguanosine (8-oxo-dG) is a specific marker of ROS-driven DNA-damage, and its accumulation makes m(5)C a hotspot for mutations. It is unknown how m(5)C and 8-oxo-dG correlate with the malignancy of gliomas. We analyzed the total contents of m(5)C and 8-oxo-dG in DNA from tumor tissue and peripheral blood samples from brain glioma patients. We found an opposite relationship in the amounts of m(5)C and 8-oxo-dG, which correlated with glioma grade in the way that low level of m(5)C and high level of 8-oxo-dG indicated increased glioma malignancy grade. Our results could be directly applied to patient monitoring and treatment protocols for gliomas, as well as bolster previous findings, suggesting that spontaneously generated ROS react with m(5)C. Because of the similar mechanisms of m(5)C and guanosine oxidation, we concluded that 8-oxo-dG could also predict glioma malignancy grade and global DNA demethylation in cancer cells. MDPI 2019-09-11 /pmc/articles/PMC6770701/ /pubmed/31514401 http://dx.doi.org/10.3390/cells8091065 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Barciszewska, Anna-Maria Giel-Pietraszuk, Małgorzata Perrigue, Patrick M. Naskręt-Barciszewska, Mirosława Total DNA Methylation Changes Reflect Random Oxidative DNA Damage in Gliomas |
title | Total DNA Methylation Changes Reflect Random Oxidative DNA Damage in Gliomas |
title_full | Total DNA Methylation Changes Reflect Random Oxidative DNA Damage in Gliomas |
title_fullStr | Total DNA Methylation Changes Reflect Random Oxidative DNA Damage in Gliomas |
title_full_unstemmed | Total DNA Methylation Changes Reflect Random Oxidative DNA Damage in Gliomas |
title_short | Total DNA Methylation Changes Reflect Random Oxidative DNA Damage in Gliomas |
title_sort | total dna methylation changes reflect random oxidative dna damage in gliomas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770701/ https://www.ncbi.nlm.nih.gov/pubmed/31514401 http://dx.doi.org/10.3390/cells8091065 |
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